Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
Scopus CiteScore – 3.4 (CiteScore Tracker 3.4)
Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 7, July, p. 873–877

doi: 10.17219/acem/117685

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Experimental studies on the protective effects of the overexpression of lentivirus-mediated sirtuin 6 on radiation-induced lung injury

Jiying Wang1,A,B,C,D,E,F, Yong Cai2,A,E,F, Zhaoying Sheng2,B,C,D,F

1 Department of Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China

2 Department of Radiation Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, China

Abstract

Background. Sirtuin 6 (SIRT6) can increase the radiosensitivity of non-small cell lung cancer and exert protective effects on radiation-induced lung injury.
Objectives. To investigate protective effects of SIRT6 overexpression on radiation-induced lung injury in rats.
Material and Methods. Male Wistar rats (n = 72) were randomly divided into 3 groups. Models were made by radiating both lungs with a 6MV X linear accelerator. Each group was injected through the tail vein with normal saline (the control group and radiation group) and lentivirus carrying overexpressed SIRT6 (the Lent-SIRT6 group) on the same day as the modeling. Routine blood indexes (white blood cells (WBC), red blood cells (RBC), neutrophils and lymphocytes) were recorded; the rats were sacrificed and their lung tissues taken; pathological changes in the lungs were evaluated using hematoxylin and eosin (H&E) staining; and tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL-1β) were detected with enzyme-linked immunosorbent assay (ELISA) 8 weeks after radiotherapy.
Results. The lung structure including alveolar walls and interstitium in the control group was normal, but the alveolar walls in the radiation group were obviously thickened and a large amount of hyperplastic fibrous tissue was found in the alveolar interstitium. The thickness and interstitial fibrosis of the alveolar walls were more alleviated in the Lent-SIRT6 group than in the radiation group. Compared with those in the control group, the respiratory rates, levels of TNF-α and IL-6 in serum, neutrophils and levels of TNF-α, IL-6 and IL-1β in the liver all were increased, while WBCs and lymphocytes were decreased in the radiation group. The respiratory rates, levels of TNF-α and IL-6 in serum, neutrophils and levels of TNF-α, IL-6 and IL-1β in the liver were all decreased, and WBCs and lymphocytes were increased after injection with lentivirus carrying overexpressed SIRT6.
Conclusion. Sirtuin 6 inhibits inflammation and alleviates radioactive pneumonia and lung injury. Therefore, SIRT6 can exert certain protective effects on lung injury.

Key words

inflammation, lung injury, sirtuin 6, radioactive pneumonia

References (13)

  1. Finkel T, Deng CX, Mostoslavsky R. Recent progress in the biology and physiology of sirtuins. Nature. 2009;460(7255):587.
  2. Yao T, Quan L. Research progress of MMP-9, HIF-1 and SIRT1 in acute lung injury. Chin J Emerg Med. 2016;25(3):384–388.
  3. Imanishi S, Hayashi R, Ichikawa T, et al. SRT1720, a SIRT1 activator, aggravates bleomycin-induced lung injury in mice. Food Nutr Sci. 2012;3(2):157–163.
  4. Li T, Zhang J, Feng J, et al. Resveratrol reduces acute lung injury in a LPS-induced sepsis mouse model via activation of Sirt1. Mol Med Rep. 2013;7(6):1889–1895.
  5. Smith LM, Wells JD, Vachharajani VT, Yoza BK, McCall CE, Hoth JJ. SIRT1 mediates a primed response to immune challenge after traumatic lung injury. J Trauma Acute Care Surg. 2015;78(5):1034–1038.
  6. Cai Y, Sheng ZY, Liang SX. Radiosensitization effect of overexpression of adenovirus-mediated SIRT6 on A549 non-small cell lung cancer cells. Asian Pac J Cancer Prev. 2014;15(17):7297–7301.
  7. Luhua W, Xiaolong F, Ming C, et al. Diagnosis and treatment of radiation-induced lung injury. Chin J Rad Oncol. 2015;24(1):4–9.
  8. Zhixi Y, Chuanxi C. Analysis of clinical characteristics of radiation-induced lung injury in patients with non-small cell lung cancer. Chin J Clin Oncol Rehabil. 2015;22(1):7–8.
  9. Jing J, Bingsheng W. Research progress of cytokines in radiation-induced lung injury. J Mod Oncol. 2016;24(3):488–491.
  10. Cuiying Z, Hong S, Yanyang W, et al. Relationship among expression of TGFβ1, α-SMA, Col1a1 and FN mRNA in lung tissues of mice with radia-tion-induced lung injury. J Ningxia Univ. 2015;6(5):33–36.
  11. Xin M, Qian Z, Yingmei L, et al. Experimental study on the protective effects of amifostine on radiation-induced lung injury in rats. Chin Oncol. 2013;23(1):1–9.
  12. Weihua Y, Chunhua N, Hongmei X, et al. Experimental study on the effect of thalidomide on radiation-induced lung injury. J Mod Oncol. 2016;24(19):3012–3016.
  13. Tingzhen X, Qichu Y, Jiaojiao A. Effect of polygonum cuspidate on plasma levels of TGF-β IL-6 and ACE in rats with radiation-induced lung injury. Chin J Trad Med Sci Technol. 2016;23(1):32–36.
© Wroclaw Medical University