Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 7, July, p. 853–856

doi: 10.17219/acem/121010

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Is serum anti-Müllerian hormone (AMH) assay a satisfactory measure for ovarian reserve estimation? A comparison of serum and peritoneal fluid AMH levels

Marta Kostrzewa1,A,B,C,D,E, Ewa Głowacka2,B,C, Tomasz Stetkiewicz1,C,E,F, Mariusz Grzesiak3,C,E, Krzysztof Szyłło1,E,F, Grzegorz Stachowiak1,A,E,F, Jacek Radosław Wilczyński4,A,E,F

1 Department of Operative Gynecology and Gynecologic Oncology, Polish Mother’s Memorial Hospital Research Institute, Łódź, Poland

2 Laboratory Diagnostics Center, Polish Mother’s Memorial Hospital Research Institute, Łódź, Poland

3 Department of Perinatology, Obstetrics and Gynecology, Polish Mother’s Memorial Hospital Research Institute, Łódź, Poland

4 Department of Gynecological Surgery and Oncology, Medical University of Lodz, Poland


Background. Clinical cases have been reported with women who got pregnant with confirmed low serum anti-Müllerian hormone (AMH) concentrations, thus demonstrating that low serum AMH concentration cut-points could be fairly specific for poor ovarian response (POR) to gonadotrophin stimulation, but not for pregnancy. That observation prompted the question whether serum AMH concentration accurately corresponded to the whole amount of AMH secreted by granulosa cells.
Objectives. To measure AMH levels in peritoneal fluid and their correlations with serum AMH concentrations.
Material and Methods. The reported study involved 48 female patients, aged 18–40 years, diagnosed with benign ovarian cysts and qualified for a laparoscopic cystectomy. Prior to surgery, the ovarian reserve was assessed using serum AMH concentration assay. The peritoneal fluid was also collected during the laparoscopy and AMH concentrations in peritoneal fluid were measured.
Results. The AMH present in the peritoneal fluid strongly correlated with AMH levels in blood serum (r = 0.54; p < 0.001) and higher serum AMH concentrations corresponded to higher AMH concentrations in the peritoneal fluid. There was also a significant correlation between AMH levels in serum and in peritoneal fluid, collected from patients with endometrioma and other benign cysts (r = 0.61; p = 0.001 vs r = 0.43; p = 0.03).
Conclusion. The AMH is present in the peritoneal fluid and its concentrations significantly correlate with AMH levels in serum. The assessment of AMH concentration in the peritoneal fluid may be a valuable complement to the evaluation of ovarian reserve and the diagnosis of infertility after adnexal surgery.

Key words

anti-Müllerian hormone, peritoneal fluid, ovarian reserve, endometrioma, fertility

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