Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 7, July, p. 793–801

doi: 10.17219/acem/121929

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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miR-21-5p protects hippocampal neurons of epileptic rats via inhibiting STAT3 expression

Xiaolei Zhang1,A,D,F, Xianfeng Li2,A,C,D,F, Bin Li3,B,F, Chengfeng Sun4,B,F, Peng Zhang5,E,F

1 Department of Neurology, Jining Combine Traditional Chinese and Western Medicine Hospital, China

2 Department of Neurosurgery, Penglai People’s Hospital, Yantai, China

3 Second Department of Neurology, The Second People’s Hospital of Liaocheng, China

4 Department of Traditional Chinese Medicine, Linyi County Hospital of Traditional Chinese Medicine, Dezhou, China

5 Department of Neurology, Gaomi People’s Hospital, Weifang, China

Abstract

Background. Epilepsy is a common chronic neurological disorder worldwide.
Objectives. To investigate the effects of miR-21-5p and signal transducer and activator of transcription-3 (STAT3) expressions on the apoptosis of hippocampal neurons in epileptic rats.
Material and Methods. We created a rat model of epilepsy and examined the relationship between miR-21-5p and STAT3 using a bioinformatics website and dual the luciferase reporter (DLR) assay. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were used to detect the expression levels of miR-21-5p and STAT3 in hippocampal neurons as well as the protein expression levels of cleaved caspase-3, Bax and Bcl-2, which were related to apoptosis of hippocampal neuron. The apoptosis and survival of hippocampal neurons were detected using TUNEL and Nissl staining. Expressions of inflammatory factors interleukin (IL)-6 and tumor necrosis factor α (TNF-α) in serum were examined with enzyme-linked immunosorbent assay (ELISA).
Results. miR-21-5p can bind to STAT3. Compared with the miR-21-5p inhibitor negative control (NC) group, the expression levels of caspase-3 and Bax were higher and the expression level of Bcl-2 was lower in the miR-21-5p inhibitor group, whereas the caspase-3 and Bax levels were lower and Bcl-2 level was higher in the si-STAT3 (interfering STAT3 gene expression by transfecting small interfering RNA) group (all p < 0.05). Treatment with miR-21-5p inhibitor can lead to significant loss and apoptosis of hippocampal neurons, while interfering with STAT3 expression can reduce the loss and apoptosis of the neurons (all p < 0.05). Compared with the miR-21-5p inhibitor NC group, the level of IL-6 was lower in the si-STAT3 group and higher in the miR-21-5p inhibitor group (both p < 0.05).
Conclusion. miR-21-5p can inhibit STAT3 expression and reduce apoptosis and loss of hippocampal neurons and IL-6 level, thereby achieving protective effects on hippocampal neurons of epileptic rats.

Key words

epilepsy, STAT3, hippocampal neuron, miR-21-5p, epileptic rats

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