Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 5, May, p. 597–602

doi: 10.17219/acem/121508

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Macrophage migration inhibitory factor gene polymorphisms as exacerbating factors of apical periodontitis

Andrea Freer-Rojas1,A,B,D, Luis Carlos Martínez-Garibay1,A,B,D, Fernando Torres-Méndez1,A,C, Claudia Edith Dávila-Pérez1,A,C, Gabriel Alejandro Martínez-Castañón2,E,F, Nuria Patiño-Marín2,E,F, Jorge Alejandro Alegría-Torres3,4,A,C,D,E,F

1 Department of Endodontics, Dental School, Autonomous University of San Luis Potosí, Mexico

2 Clinical Research Laboratory, Program of Doctorate in Dental Sciences, Autonomous University of San Luis Potosí, Mexico

3 Department of Pharmacy, University of Guanajuato, Mexico

4 Molecular Nutrition Research Laboratory, LIMON, University of Central Mexico UCEM, San Luis Potosí, Mexico


Background. Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5–8 and -173G>C); however, so far there are no reports of studies related to oral health.
Objectives. To genotype the -794 CATT5–8 and -173G>C MIF polymorphisms in Mexican patients with apical periodontitis as a genetic risk of exacerbation.
Material and Methods. The study involved 120 patients with apical periodontitis: 60 with a diagnosis of acute apical periodontitis (Group A) and 60 without previous episodes of exacerbation (Group B). Allelic discrimination was performed from peripheral blood DNA; the repeat polymorphism -794 CATT5–8 was genotyped with sequencing, while the -173G>C polymorphism was determined using real-time polymerase chain reaction (RT-PCR) using TaqMan probes. The associations between MIF polymorphisms, haplotypes and the risk of exacerbated apical periodontitis were assessed.
Results. The allele CATT7 was associated with the risk of a stage of acute inflammation (OR = 4.13; 95% CI = 1.82–9.63; p =< 0.001). Regarding the -173G >C polymorphism, a process of inflammation exacerbation was only associated with the CC genotype (OR = 4.1; 95% CI = 1.02–20.84; p = 0.045). The analysis of the haplotype showed that the combination CATT7/C increases the risk of exacerbation of apical periodontitis (OR = 3.57; 95% CI = 1.038–13.300; p = 0.021).
Conclusion. The polymorphisms -794 CATT5–8 and -173G>C MIF seem to significantly influence the development of a state of exacerbated inflammation in patients with apical periodontitis.

Key words

apical periodontitis, genetic polymorphisms, macrophage migration inhibitory factor

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