Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 1, January, p. 5–11

doi: 10.17219/acem/111809

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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An experimental study on the use of lycopene to prevent infertility due to acute oxidative ovarian damage caused by a single high dose of methotrexate

Can Turkler1,A,B,C,D,E,F, Taylan Onat2,C,E, Engin Yildirim3,D,E,F, Selcuk Kaplan4,D,E,F, Gulce Naz Yazici5,A,B,F, Renad Mammadov6,A,B,C, Mukadder Sunar7,B,E,F

1 Department of Gynecology and Obstetrics, Faculty of Medicine, Erzincan Binali Yıldırım University, Turkey

2 Department of Gynecology and Obstetrics, Faculty of Medicine, Bozok University, Yozgat, Turkey

3 Department of Gynecology and Obstetrics, Faculty of Medicine, Hitit University, Corum, Turkey

4 Department of Gynecology and Obstetrics, Faculty of Medicine, Adıyaman University, Turkey

5 Department of Histology and Embryology, Faculty of Medicine, Erzincan Binali Yıldırım University, Turkey

6 Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yıldırım University, Turkey

7 Department of Anatomy, Faculty of Medicine, Erzincan Binali Yıldırım University, Turkey


Background. Methotrexate (MTX) is an antineoplastic agent, which increases the level of reactive oxygen species (ROS) and decreases the level of antioxidants. Lycopene, is a potent antioxidant, which is used because of its protective effect against tissue damage.
Objectives. The aim of this study was to determine the effect of lycopene on ovarian MTX-induced injury in rats.
Material and Methods. Rats (n = 36) were randomly divided into 3 equal groups: a group with MTX only (MG, n = 12), a group with lycopene and MTX (LMG, n = 12), and a healthy control group (HCG, n = 12). Then, malondialdehyde (MDA), myeloperoxidase (MPO) and total glutathione (tGSH) levels and histopathological findings were examined in the ovaries of rats. Apart from the histopathological and biochemical evaluation, the reproductive performance of the experimental groups was also examined.
Results. Our study demonstrated that, in ovarian tissues of rats administered MTX, there was a decrease in the levels of tGSH, while MDA and MPO were increased, but it is observed that these ratios are reversed in the LMG (p < 0.05). It also has been proven that a single, high-dose use of MTX causes infertility in female rats, prolongs the gestation period and reduces the number of offspring.
Conclusion. Lycopene pretreatment ameliorates the MTX induced ovarian injury and infertility in rats through its antioxidative activities.

Key words

methotrexate, infertility, oxidative stress, rat, lycopene

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