Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 8, August, p. 1043–1050

doi: 10.17219/acem/94392

Publication type: original article

Language: English

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Identification of alternative splicing and lncRNA genes in pathogenesis of small cell lung cancer based on their RNA sequencing

Youming Lei1,A,D, Yunfei Shi1,A,B, Jin Duan1,B,C, Yinqiang Liu1,C,E, Guoli Lv1,C,E, Rou Shi1,B,E, Fujun Zhang1,B,E, Qingmei Yang1,B,C, Wei Zhao1,A,F

1 The First Affiliated Hospital of Kunming Medical University, China

Abstract

Background. The molecular mechanisms involved in small-cell lung cancer (SCLC) are largely unknown. Recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a critical role.
Objectives. There is an urgent need for suitable molecular biomarkers for SCLC diagnosis and for assessing patient prognosis.
Material and Methods. In this study, we used public databases to identify mRNA-like candidate lncRNAs. A multi-step computational approach was used to construct a functional SCLC lncRNAs-mediated competing with endogenous RNA (ceRNA) network (LMCN) by integrating genome-wide lncRNAs and mRNA expression profiles, miRNA-target interactions, functional analyses, and clinical survival analyses.
Results. The results revealed the significance of lncRNAs interactions with ceRNAs in SCLC, indicating that integration of expression profiles and alternative splicing could be used to identify biomarkers and the underlying pathological changes. The following genes: EPB41L4A-AS1, HOXA-AS2, XIST, DLEU2, FGD5-AS1, ALMS1-IT1, SNHG12, MIR17HG, MIR4720, and SCARNA10 in cluster, as well as shared alternative splicing events, were considered to be critical genes.
Conclusion. Olfactory transduction and endocytosis were the top-enriched pathways in SCLC. The selected cluster, including critical genes, might also be a potential pathway of SCLC pathogenesis. As a result, this research provides the perspective information to explore the potential critical genes and its pathways in SCLC therapy.

Key words

alternative splicing, small-cell lung cancer (SCLC), critical genes

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