Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 7, July, p. 973–980

doi: 10.17219/acem/99613

Publication type: original article

Language: English

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Co-expression of the aryl hydrocarbon receptor and estrogen receptor in the developing teeth of rat offspring after rat mothers’ exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin and the protective action of α-tocopherol and acetylsalicylic acid

Maciej Dobrzyński1,A,B,C,D,E,F, Piotr Kuropka2,B,C,D,E,F, Anna Leśków3,C,D, Katarzyna Herman1,C,D, Małgorzata Tarnowska3,C,D, Rafał J. Wiglusz4,5,E,F

1 Department of Conservative Dentistry and Pedodontics, Wroclaw Medical University, Poland

2 Department Histology and Embriology, Wroclaw University of Environmental and Life Sciences, Poland

3 Department of Nervous System Diseases, Wroclaw Medical University, Poland

4 Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wrocław, Poland

5 Department of Physico-Chemistry of Microorganisms, Institute of Genetics and Microbiology, University of Wrocław, Poland

Abstract

Background. Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can cause adverse effects in many organs. Toxic effects are caused due to the formation of a TCDD complex with the cytoplasmatic aryl hydrocarbon receptor (AhR), whose mechanism of action is similar to that of the estrogen receptor (ER). Some substances, including α-tocopherol (E) and acetylsalicylic acid (ASA), can reduce the toxic effects of TCDD in offspring.
Objectives. The objective of this study was to evaluate the co-expression of AhR and ER in the incisors of rat offspring whose mothers were exposed to TCDD, using immunohistochemical and histological techniques. Moreover, the possible protective role of E and ASA was investigated.
Material and Methods. Four groups of 2-day-old rat offspring, whose mothers were intoxicated by TCDD before mating, were established: control group (C), TCDD group, TCDD+E group and TCDD+ASA group.
Results. In the TCDD group, there was an increase in ER expression and a decrease in AhR expression in comparison with the C group. In the TCDD+E and TCDD+ASA groups, there was a weak or negative ER expression and slightly stronger expression of AhR than in the TCDD group.
Conclusion. The co-expression of AhR and ER during tooth development suggests the role of AhR and ER in the control of this process. Both receptors are also involved in the process of detoxification of TCDD. The increase in AhR in TCDD+E and TCDD+ASA groups indicate a preventive action of antioxidant and antiinflammatory pharmaceutics, which may limit negative effects of TCDD.

Key words

rat, estrogen receptor, tooth, dioxin, aryl hydrocarbon receptor

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