Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
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Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 5, May, p. 671–678

doi: 10.17219/acem/94135

Publication type: original article

Language: English

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Assessment of apelin, apelin receptor, resistin, and adiponectin levels in the primary tumor and serum of patients with esophageal squamous cell carcinoma

Dorota Diakowska1,A,B,C,D,E,F, Krystyna Markocka-Mączka2,A,B,E,F, Mirosław Nienartowicz2,B,E,F, Joanna Rosińczuk1,C,E,F, Małgorzata Krzystek-Korpacka3,A,C,D,E,F

1 Department of Nervous System Diseases, Faculty of Health Science, Wroclaw Medical University, Poland

2 Department of Gastrointestinal and General Surgery, Wroclaw Medical University, Poland

3 Department of Medical Biochemistry, Wroclaw Medical University, Poland


Background. Disturbances in adipokine secretion are associated with the risk of cancer growth and progression.
Objectives. The aim of the study was to evaluate the mRNA expression and protein levels of apelin, the apelin receptor, resistin, and adiponectin in the tumor tissues of surgically treated esophageal squamous cell carcinoma (ESCC) patients. Concentrations of serum adipokines were assessed in relation to ESCC progression.
Material and Methods. The study group consisted of 53 patients with ESCC and 27 controls. In the ESCC group, 27 patients were surgically treated and 26 were treated with palliative procedures. RT-PCR and ELISA tests were used to measure the mRNA expression and protein level of adipokines in tissues and their concentration in serum.
Results. We found that mRNA expression and protein concentrations of apelin, the apelin receptor and resistin were significantly higher in tumor tissue than in control tissue. The protein concentration of apelin were significantly increased in the tumors of patients with lymph node metastasis (p < 0.005). Circulating levels of apelin, the apelin receptor and resistin were significantly higher in the cancer patients than in controls (p < 0.05 for all). The concentration of serum apelin receptor significantly decreased in patients with stage IV cancer, the presence of lymph node or distant metastasis (p < 0.05).
Conclusion. Apelin may participate in lymphangiogenesis and the progression of ESCC. The apelin receptor is intensely produced in the early stage of cancer development and it may take part in the carcinogenic processes of ESCC.

Key words

adipokines, apelin, esophageal squamous cell carcinoma, apelin receptor

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