Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
Scopus CiteScore – 3.4 (CiteScore Tracker 3.4)
Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 4, April, p. 431–438

doi: 10.17219/acem/92563

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Impact of fabrics from transgenic flax on cultures of skin cells

Kazimierz Gąsiorowski1,A,C,E,F, Tomasz Gębarowski1,B,C,D, Helena Moreira1,B,C, Anna Kulma2,E,F, Michał Szatkowski2,E, Jan Szopa2,A,E

1 Department of Basic Medical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Poland

2 Department of Genetic Biochemistry, Faculty of Biotechnology, University of Wroclaw, Poland


Background. The development of a new type of wound dressing material that can support skin regeneration is an important challenge to improve treatment of chronic, non-healing wounds.
Objectives. The objective of this study was to compare the impact of flax fabrics from transgenic plants overexpressing phenolic acids and flavonoids (W92) and polyhydroxybutyrate (M48), as well as fabric from non-transgenic plant (Nike) on cultures of human skin cells.
Material and Methods. Flax fabric pieces as well as water extracts from the fabrics were co-cultured with human skin cells: keratinocytes, fibroblasts, dermal microvascular endothelial cells, and with monocytoid cell line (THP1) for 48 h. Cell viability and proliferation were assessed with the sulforhodamine B colorimetric assay. Intracellular reactive oxygen species (ROS) was estimated with the 2’7 dichlorodihydrofluorescein diacetate (DCFH-DA) oxidation method. Endothelial cell migration was measured with the scratch assay. The results were compared with the multi-criteria analysis (MCA) procedure.
Results. Tested flax fabrics released flavonoids and polyhydroxybutyrate to cell culture media, as it was determined by means of the high performance liquid chromatography (HPLC) method. Fabrics from transgenic plants W92 and M48 promoted proliferation of keratinocytes and fibroblasts. Water extracts from flax fabric diminished the proliferation of monocytoid cells, decreased oxidative burst in activated THP1 cells and accelerated the velocity of dermal microvascular cell migration. The MCA proved that the sum of beneficial effects estimated in human skin cell cultures was higher (by 47% and by 34% with W92 and M48, respectively) than that of non-transgenic flax fabric (Nike).
Conclusion. The W92 and M48 fabrics should be further studied as candidates for elaboration of new types of bandages, able to improve skin wound healing.

Key words

flax fabric, skin cell cultures, flax biotechnology, transgenic flax fabrics

References (23)

  1. Szopa J, Wrobel-Kwiatkowska M, Kulma A, et al. Chemical composition and molecular structure of fibers from transgenic flax producing polyhydroxybutyrate, and mechanical properties and platelet aggregation of composite materials containing these fibers. Compos Sci Technol. 2009;69(14):2438–2446.
  2. Zuk M, Kulma A, Dyminsk Lamalice L, Le Boeuf F, Huot J. Endothelial cell migration during angiogenesis. Circ Res. 2007;100(6):782–794.
  3. Lorenc-Kukula K, Amarowicz R, Oszmianski J, et al. Pleiotropic effect of phenolic compounds content increases in transgenic flax plant. J Agric Food Chem. 2005;53(9):3685–3692.
  4. Kunert-Keil C, Gredes T, Meyer A, Wrobel-Kwiatkowska M, Domi­niak M, Gedrange T. The survival and proliferation of fibroblasts on biocomposites containing genetically modified flax fibers: An in vitro study. Ann Anat. 2012;194(6):513–517.
  5. Skorkowska-Telichowska K, Zuk M, Kulma A, et al. New dressing materials derived from transgenic flax products to treat long-standing venous ulcers: A pilot study. Wound Repair Regen. 2010;18(2):168–179.
  6. Skorkowska-Telichowska K, Czemplik M, Kulma A, Szopa J. The local treatment and available dressings designed for chronic wounds. J Am Acad Dermatol. 2013;68(4):e117–e126.
  7. Beck B, Iversen P, Shashegy A. Combining information for quantitative decision-making in drug development. JMCDA. 2014;21:139–151.
  8. Nutt DJ, King LA, Phillips LD. Drug harms in the UK: A multicriteria decision analysis. Lancet. 2010;376(9752):1558–1565.
  9. Wróbel M, Zebrowski J, Szopa J. Polyhydroxybutyrate synthesis in transgenic flax. J Biotechnol. 2004;107(1):41–54.
  10. Wróbel-Kwiatkowska M, Zebrowski J, Starzycki M, Oszmianski J, Szopa J. Engineering of PHB synthesis causes improved elastic properties of flax fibers. Biotechnol Prog. 2007;23(1):269–277.
  11. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc. 2006;1(3):1112–1116.
  12. Eruslanov E, Kusmartsev S. Identification of ROS using oxidized DCFDA and flow-cytometry. Methods Mol Biol. 2010;594:57–72.
  13. Liang C-C, Park AY, Guan J-L. In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007;2(2):329–333.
  14. Cheng S, Chen GQ, Leski M, Zou B, Wang Y, Wu Q. The effect of D,L-beta-hydroxybutyric acid on cell death and proliferation in L929 cells. Biomaterials. 2006;27(20):3758–3765.
  15. Telgenhoff D, Shroot B. Cellular senescence mechanisms in chronic wound healing. Cell Death Differ. 2005;12(7):695–698.
  16. Seah CC, Phillips TJ, Howard CE, et al. Chronic wound fluid suppresses proliferation of dermal fibroblasts through a Ras-mediated signaling pathway. J Invest Dermatol. 2005;124(2):466–474.
  17. Usui ML, Mansbridge JN, Carter WG, Fujita M, Olerud JE. Keratinocyte migration, proliferation, and differentiation in chronic ulcers from patients with diabetes and normal wounds. J Histochem Cytochem. 2008;56(7):687–696.
  18. Pendyala S, Gorshkova IA, Usatyuk PV, et al. Role of Nox4 and Nox2 in hyperoxia-induced reactive oxygen species generation and migration of human lung endothelial cells. Antioxid Redox Signal. 2009;11(4):747–764.
  19. Clanchy FIL, Holloway AC, Lari R, Cameron PU, Hamilton JA. Detection and properties of the human proliferative monocyte subpopulation. J Leukoc Biol. 2006;79(4):757–766.
  20. Winkler C, Ueberall F, Fuchs D. In vitro testing for anti inflammatory properties of compounds. Clin Chem. 2006;52(6):1201–1202.
  21. Goel G, Makkar HPS, Francis G, Becker K. Phorbol esters: Structure, biological activity, and toxicity in animals. Int J Toxicol. 2007;26(4):279–288.
  22. Jiang F, Zhang Y, Dusting GJ. NADPH oxidase-mediated redox signaling: Roles in cellular stress response, stress tolerance, and tissue repair. Pharmacol Rev. 2011;63(1):218–242.
  23. Lamalice L, Le Boeuf F, Huot J. Endothelial cell migration during angiogenesis. Circ Res. 2007;100(6):782–794.