Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 7, July, p. 907–911

doi: 10.17219/acem/70810

Publication type: original article

Language: English

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Examination of the antioxidant effects of pre-HSG melatonin use on ovarian surface epithelium in rats: An experimental study

Esra Saygılı Yılmaz1,A,B,C,D,E,F, Tansel Sapmaz2,3,B,C, Halil Kazgan1,A,B, Şule Menziletoglu Yildiz4,A,D, Derya Kocamaz4,A,D, Nusret Akpolat5,C,E, Ekrem Sapmaz1,C,F

1 Department of Gynecology and Obstetrics, Adana Numune Training and Research Hospital, Turkey

2 Department of Histology and Embryology, Institute of Medical Sciences, Imperial School of Medicine, Istanbul, Turkey

3 Department of Histology and Embryology, Faculty of Medicine, Firat University, Elazig, Turkey

4 Blood Center of Balcali Hospital, Faculty of Medicine, Cukurova University, Adana, Turkey

5 Department of Pathology, Faculty of Medicine, Inonu University, Malatya, Turkey

Abstract

Background. There is no study of whether the dysplastic changes in the ovarian surface epithelium of X-ray-exposed rats during hysterosalpingography (HSG) decrease or not with the use of Lipiodol and melatonin given both intraperitoneally (i.p.) and into the suspensorium ovarii.
Objectives. We investigated the restorative effects of melatonin and Lipiodol administration during the HSG procedure on the dysplastic changes in the ovarian surface epithelium of X-ray-exposed rats.
Material and Methods. A total of 50 Wistar rats with regular estrous cycles were randomly divided into 5 groups. Group 1 was the control group. In other groups, X-ray was applied (group 2), 0.1 mL Lipiodol was applied to each uterine horn (group 3), 20 mg/kg intraperitoneal melatonin application was followed by 0.1 mL Lipiodol administration to each uterine horn after 15 min (group 4), and 20 mg/kg melatonin was administered to the ligamentum suspensorium ovarii, followed by 0.1 mL Lipiodol application to each uterine horn after 15 min (group 5). The rats in groups 2–5 were exposed to whole body radiation 3 times. After 3 h, the abdomens of all rats were reopened and left oophorectomy was performed.
Results. The presence of nucleoli and mitosis values were found similar among the groups. All other parameters were significantly higher in group 2 compared to other groups, except for the presence of nucleoli and mitosis values (p < 0.05). The presence of hyperchromasia and the total score were found to be the highest in group 2, followed by group 3, when compared to other groups (p < 0.05). It was detected that the detrimental effects of X-ray exposure diminished with Lipiodol use, and were further reduced by the use of melatonin in combination.
Conclusion. We suggest that the use of melatonin and Lipiodol during HSG may prevent the carcinogenic changes exerted by radiation on the ovarian surface epithelium.

Key words

melatonin, hysterosalpingography, rats, radiation, Lipiodol

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