Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 6, June, p. 765–772

doi: 10.17219/acem/68901

Publication type: original article

Language: English

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Quality control of riboflavin-treated platelet concentrates using Mirasol® PRT system: Polish experience

Elżbieta Lachert1,A,B,C,D, Jolanta Kubis1,A,B,C,D, Jolanta Antoniewicz-Papis1,A,B,C,D, Aleksandra Rosiek1,C, Jolanta Woźniak1,B,C, Dariusz Piotrowski2,B, Zofia Przybylska2,B, Agata Mikołowska1,A,B,C,D, Susanne Marschner3,E,F, Magdalena Łętowska1,E,F

1 Institute of Hematology and Transfusion Medicine, Warszawa, Poland

2 Regional Blood Transfusion Center, Warszawa, Poland

3 Terumo BCT Biotechnologies, Lakewood, USA

Abstract

Background. The quality of platelet concentrates (PCs) is affected by preparation, storage, the type of container, and pathogen reduction technology (PRT). The Mirasol® Pathogen Reduction Technology (PRT) system (Terumo BCT Inc., Lakewood, USA), which uses riboflavin and ultraviolet (UV) light, has recently been proven effective against bacteria, viruses, parasites, and leukocytes.
Objectives. The aim of the study was to evaluate the effect of the Mirasol® PRT system, based on riboflavin and UV light exposure, on the most common in vitro platelet quality parameters of PCs prepared from whole blood-derived buffy coats.
Material and Methods. The study included 15 trials (n = 15). For each trial, 2 PCs were used: 1 for treatment with the Mirasol® PRT system (M) and 1 for a control (C). In the M group, PCs were illuminated. In the C group, saline solution was added. PCs from groups M and C were stored at 20–24°C, with agitation. Samples were collected on days 1, 3 and 5 to determine platelet concentration, total platelet count/unit, mean platelet volume (MPV), power of hydrogen (pH), glucose and beta-thromboglobulin concentration (BTG), hypotonic shock response (HSR), aggregation, CD42b and CD62P expression, pCO2, and pO2.
Results. No significant differences in HSR or CD42b expression were observed between groups M and C. All pH values were stable during the whole storage period (7.1–7.5). On storage day 1, CD62P expression in group C was significantly higher than in group M. In the Mirasol® group, significantly higher glucose consumption was noted on storage days 3 and 5. On day 5, a 2–3-fold increase in BTG was observed in both groups as compared to day 1; on day 5, BTG concentration was 32% higher in group M than in group C. On all storage days, pCO2 was comparable in groups M and C; lower pO2 values were reported for group M.
Conclusion. In vitro results demonstrated that pH, HSR, aggregation, CD42b antigen expression, and MPV and platelet count parameters were comparable in groups M and C.

Key words

pathogen, platelets, inactivation

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