Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 5, May, p. 591–597

doi: 10.17219/acem/68896

Publication type: original article

Language: English

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The protective effect of Gingko biloba in a rat model of ovarian ischemia/reperfusion injury: Improvement in histological and biochemical parameters

Nuri Yildirim1,A,D,F, Deniz Simsek1,C,D, Semir Kose2,C,E, Alkim Gulsah Sahingoz Yildirim3,B,E, Cagri Guven1,C,E, Gurkan Yigitturk4,B,E, Oytun Erbas5,A,E,F

1 Department of Obstetrics and Gynecology, Ege University, Izmir, Turkey

2 Department of Obstetrics and Gynecology, Dokuz Eylul University, Izmir, Turkey

3 Department of Obstetrics and Gynecology, Tepecik Maternity and Research Hospital, Izmir, Turkey

4 Department of Histology and Embryology, Ege University, Izmir, Turkey

5 Department of Physiology, Bilim University, Istanbul, Turkey

Abstract

Background. Ovarian torsion is one of the most common gynecological emergencies, which especially affects women of reproductive age.
Objectives. We aimed to evaluate the effect of Ginkgo biloba (GB) supplementation in ovarian ischemia/ reperfusion injury in an experimental torsion/de-torsion rat model.
Material and Methods. This study was carried out in the Ege University Faculty of Medicine in Izmir, Turkey. Thirty mature female Sprague-Dawley albino rats were randomly divided into 5 groups: in Group 1 (control), the abdominal wall was only opened and closed; in the torsion group (Group 2), ischemia was induced for 3 h, using atraumatic vascular clips to create a torsion model; in the torsion/GB group (Group 3), the rats were given 80 mg/kg (oral gavage) of GB 30 min before torsion was induced and the torsion model was formed; in the torsion/de-torsion group (Group 4), the rats underwent 3 h of ischemia and then the vascular clips were removed and reperfusion took place for 3 h; in the torsion/de-torsion/GB group (Group 5), the rats underwent 3 h of ischemia followed by GB (oral gavage) 30 min prior to a 3-h reperfusion period. Ovarian tissue damage was evaluated by a histopathological scoring system. Ovarian tissue malondialdehyde (MDA) and plasma pentraxin-3 were measured.
Results. In comparison with the sham group, both the torsion and torsion/de-torsion groups had significantly higher scores for follicular degeneration, vascular congestion, edema, hemorrhage, and leukocyte infiltration. Ginkgo biloba significantly decreased these scores in both groups. Ovarian malondialdehyde and plasma pentraxin 3 were significantly higher both in the torsion and torsion/de-torsion groups compared with the sham group. Ginkgo biloba decreased these levels significantly both in the torsion/GB and torsion/de-torsion/GB groups.
Conclusion. Supplementing GB during a surgical procedure decreases ischemia/reperfusion injury to an ovary in an experimental rat model based on histopathological parameters, tissue malondialdehyde, and plasma pentraxin-3 levels.

Key words

malondialdehyde, ischemia/reperfusion injury, ovarian torsion, Ginkgo biloba, pentraxin-3

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