Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.727
Index Copernicus  – 166.39
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 2, February, p. 159–163

doi: 10.17219/acem/69231

Publication type: original article

Language: English

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Urine nerve growth factor (NGF) level, bladder nerve staining and symptom/problem scores in patients with interstitial cystitis

Senol Tonyali1,A,B,C,D,E,F, Deniz Ates2,A,B,C,D,E,F, Filiz Akbiyik3,A,B,C,D,E,F, Duygu Kankaya4,A,B,C,D,E,F, Dilek Baydar2,A,B,C,D,E,F, Ali Ergen1,A,B,C,D,E,F

1 Department of Urology, Hacettepe University School of Medicine, Ankara, Turkey

2 Department of Pathology, Hacettepe University School of Medicine, Ankara, Turkey

3 Department of Medical Biochemistry, Hacettepe University School of Medicine, Ankara, Turkey

4 Department of Pathology, Ankara University School of Medicine, Ankara, Turkey


Background. Interstitial cystitis (IC)/bladder pain syndrome (BPS) is a challenging disease, affecting thousands of people all around the world, especially women. Although there have been numerous theories regarding IC/BPS etiology, the physiopathology of the disease still remains unclear and there is a lack of certain treatment.
Objectives. The aim of the study was to assess the role of nerve fibers and nerve growth factor (NGF) in the etiopathogenesis of IC/BPS symptoms by demonstrating if there is a correlation between urine NGF levels, amount of peripheral nerves in bladder mucosa and symptom severity.
Material and Methods. A prospective clinical study was conducted with 15 IC/BPS patients and 18 controls. Urine NGF levels were measured by enzyme-linked immunosorbent assay (ELISA). Bladder punch biopsies were obtained from 15 IC/BPS patients and 9 controls. Immunohistochemistry was performed for S-100 to highlight peripheral nerve twigs in bladder mucosa. The O’Leary-Sant Interstitial Cystitis Symptom and Problem Index (OSICSPI) was used to assess symptom severity and effects of the disease on the patients’ life.
Results. NGF normalized to urine creatinine (NGF/Cr) levels in IC/BPS patients were significantly higher than in controls, 0.34 ±0.22 and 0.09 ±0.08 pg/mL: mg/dL, respectively (p < 0.001). The mean symptom score in IC patients was 12.27 ±2.4 (median: 12) and the mean problem score was 10.9 ±2.3 (median: 12). The mean mucosal nerve (S-100 stained) area in the IC/BPS group was significantly higher than in the controls, 2.53 ±1.90 vs 1.0 ±0.70, respectively (p = 0.018). In correlation analyses, the NGF/Cr level in IC/BPS patients was found significantly correlated with the O’Leary-Sant IC Symptom and Problem Index scores independently (p = 0.001 and p = 0.028, respectively).
Conclusion. NGF seems to be a promising biomarker in IC/BPS. It may help clinicians in diagnoses and patient follow-up. Thus, unnecessary, expensive and invasive tests, interventions and treatments might be avoided.

Key words

nerve growth factor, bladder pain syndrome/interstitial cystitis, nerve staining

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