Advances in Clinical and Experimental Medicine
2018, vol. 27, nr 12, December, p. 1745–1749
Publication type: original article
The phospholipid fraction obtained from egg yolk reduces blood pressure increase induced by acute stress in spontaneously hypertensive rats
1 Department and Clinic of Internal and Occupational Diseases and Hypertension, Faculty of Medicine, Wroclaw Medical University, Poland
2 Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Poland
3 Division of Angiology, Faculty of Health Sciences, Wroclaw Medical University, Poland
Background. Despite continuous research, an improved understanding of the pathophysiology of hypertension and the development of new antihypertensive therapies, hypertension is still the most prevalent chronic cardiovascular disease (CVD) among adults in Western societies. Stress is a common factor related to cardiovascular morbidity. An increase in blood pressure is one of the most common reactions to stress. Chronic and acute stress have also been related to cardiovascular disorders. Polyunsaturated fatty acids (PUFAs) have attracted considerable interest as potential complementary therapy for the treatment of CVD, including hypertension. Egg yolk, rich in PUFAs and phospholipids, might be a good source of phospholipids and PUFAs.
Objectives. The current study was aimed at investigating the potential effect of the phospholipid fraction of eggs on blood pressure changes in spontaneously hypertensive rats (SHRs) as a response to acute stress.
Material and Methods. Male, 7-week-old SHRs received dietary phospholipid fraction for 12 weeks. The control animals received standard feed. At the end of the treatment, they were exposed to 40 min of white noise in order to induce acute stress. Then, blood pressure measurements were carried out under normal conditions for 12 h before and during the acute stress procedure. Blood pressure was measured by telemetry.
Results. Diastolic, systolic and mean blood pressure were significantly lower in the SHRs treated with phospholipid fraction than in the control group. The acute stressor caused a significant increase in diastolic, systolic and mean blood pressure in both the treated and untreated animals, but the increase in blood pressure as a response to the stressor was significantly less pronounced in the rats treated with phospholipid fraction than in control rats. These results show that egg phospholipids can limit a stress-induced rise in blood pressure.
Conclusion. The results of this research show that phospholipid fraction derived from egg yolk reduces stress-induced increases in blood pressure.
stress, hypertension, phospholipid fraction, hen eggs
- Geleijnse JM, Giltay EJ, Grobbee DE, Donders ART, Kok FJ. Blood pressure response to fish oil supplementation: Metaregression analysis of randomized trials. J Hypertens. 2002;20(8):1493–1499.
- Mozaffarian D, Geelen A, Brouwer IA, Geleijnse JM, Zock PL, Katan MB. Effect of fish oil on heart rate in humans: A meta-analysis of randomized controlled trials. Circulation. 2005;112(13):1945–1952.
- Pauletto P, Puato M, Caroli MG, et al. Blood pressure and atherogenic lipoprotein profiles of fish-diet and vegetarian villagers in Tanzania: The Lugalawa study. Lancet. 1996;348(9030):784–788.
- Morris MC, Sacks F, Rosner B. Does fish oil lower blood pressure? A meta-analysis of controlled trials. Circulation. 1993;88(2):523–533.
- Schiano V, Laurenzano E, Brevetti G, et al. Omega-3 polyunsaturated fatty acid in peripheral arterial disease: Effect on lipid pattern, disease severity, inflammation profile, and endothelial function. Clin Nutr. 2008;27(2):241–247.
- Kenny D, Warltier DC, Pleuss JA, Hoffmann RG, Goodfriend TL, Egan BM. Effect of omega-3 fatty acids on the vascular response to angiotensin in normotensive men. Am J Cardiol. 1992;70(15):1347–1352.
- Nestel P, Shige H, Pomeroy S, Cehun M, Abbey M, Raederstorff D. The n-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid increase systemic arterial compliance in humans. Am J Clin Nutr. 2002;76(2):326–330.
- Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: Analysis of worldwide data. Lancet. 2005;365(9455):217–223.
- Wolf-Maier K, Cooper RS, Benegas JR, et al. Hypertension prevalence and blood pressure levels in 6 European countries, Canada and the United States. JAMA. 2003;289(18):2363–2369.
- Castelli WP. Epidemiology of coronary heart disease: The Framingham Study. Am J Med. 1984;76(2A):4–12.
- Lewington S, Clarke R, Qizilbash N, Peto R, Collins R; Prospective Studies Collaboration. Age-specific relevance of usual blood pressure to vascular mortality: A meta-analysis of individual data for one million adults in 61 prospective studies. Lancet. 2002;360(9349):
- Whelton PK. Epidemiology of hypertension. Lancet. 1994;344(8915):101–106.
- Wojtyla A, Bilinski P, Jaworska-Luczak B. Regulatory strategies to ensure food and feed safety in Poland: Update review. Ann Agric Environ Med. 20110;17:215–220.
- Isomaa B, Almgren P, Tuomi T, et al. Cardiovascular morbidity and mortality associated with the metabolic syndrome. Diabetes Care. 2001;24(4):683–689.
- Cuspidi C, Ambrosioni E, Mancia G, Pessina AC, Trimarco B, Zanchetti A; APROS Investigators. Role of echocardiography and carotid ultrasonography in stratifying risk in patient with essential hypertension: The Assessment of Prognostic Risk Observational Survey. J Hypertens. 2002;20(7):1307–1314.
- Pauletto P, Sarzani R, Rappelli A, Pessina AC, Sartore S. Vascular smooth muscle cell differentiation and growth response in hypertension. In: Laragh JH, Brenner BM, eds. Hypertension: Pathophysiology, Diagnosis and Management. 2nd ed. New York, NY: Raven Press Publishers; 1995:697–709.
- Grassi G, Mancia G. Hyperadrenergic and labile hypertension. In: Lip GH, Hall J, eds. Comprehensive Hypertension. Philadelphia, PA: Mosby Elsevier; 2007:719–726.
- Grassi G, Cifkova R, Laurent S, et al. Blood pressure control and cardiovascular risk profile in hypertensive patient from Central and Eastern European countries: Results of BP-CARE study. Eur Heart J. 2011;32(2):218–225.
- Huang WY, Davidge ST, Wu J. Bioactive natural constituents from food sources: Potential use in hypertension prevention and treatment. Crit Rev Food Sci Nutr. 2014;53(6):615–630.
- Jahandideh F, Majumder K, Chakrabarti S, et al. Beneficial effects of simulated gastro-intestinal digests of fried egg and its fractions on blood pressure, plasma lipids and oxidative stress in spontaneously hypertensive rats. PLOS ONE. 2014;9(12):e115006.
- Yamada Y, Yamauchi D, Yokoo M, Ohinata K, Usui H, Yoshikawa M. A potent hypotensive peptide, novokinin, induces relaxation by AT2- and IP-receptor-dependent mechanism in the mesenteric artery from SHRs. Biosci Biotechnol Biochem. 2008;72(1):257–259.
- Appel LJ, Miller ER, Seidler AJ, Whelton, PK. Does supplementation of diet with “fish oil” reduce blood pressure? A meta-analysis of controlled clinical trials. Arch Intern Med. 1993;153(12):1429–1438.
- Calder PC. n-3 fatty acids and cardiovascular disease: Evidence explained and mechanisms explored. Clin Sci (Lond). 2004;107(1):1–11.
- Skoczyńska A, Wojakowska A, Nowacki D, et al. Unsaturated fatty acids supplementation reduces blood lead level in rats. Biomed Res Int. 2015;2015:189190.
- Zambrowicz A, Pokora M, Setner B, et al. Multifunctional peptides derived from an egg yolk protein hydrolysate: Isolation and characterization. Amino Acids. 2015;47(2):369–380.
- Preli RB, Klein KP, Herrington DM. Vascular effects of dietary L-arginine supplementation. Atherosclerosis.2002;162(1):1–15.
- Jayasooriya AP, Begg DP, Chen N, et al. Omega-3 polyunsaturated fatty acid supplementation reduces hypertension in TGR(mRen-2)27 rats. Prostaglandins Leukot Essent Fatty Acids. 2008;78(1):67–72.
- Nowacki D, Martynowicz H, Skoczyńska A, et. al. Lecithin derived from ω-3 PUFA fortified eggs decreases blood pressure in spontaneously hypertensive rats. Sci Rep. 2017;7(1):12373.