Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
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Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2017, vol. 26, nr 5, August, p. 789–793

doi: 10.17219/acem/63746

Publication type: original article

Language: English

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Total antioxidant capacity in Mediterranean β-thalassemic patients

Ioannis Tsamesidis1,A,B,C,D, Claudio Fozza2,C,D,E, Eleni Vagdatli3,B, Anastasia Kalpaka4,B, Carla Cirotto5,B, Maria Carmina Pau1,B, Antonella Pantaleo2,E, Francesco Turrini6,E, Elisavet Grigoriou7,C, Eugenia Lymperaki3,A,B,C,F

1 Department of Medicine, Section of Internal Medicine, University of Verona, Italy

2 Department of Biomedical Sciences, University of Sassari, Italy

3 Department of Medical Laboratories, Alexander Technological Educational Institute of Thessaloniki, Sindos, Greece

4 Saint Paul General Hospital, Thessaloniki, Greece

5 Blood Center, Servizio Trasfusionale, Ospedale Santissima Annunziata, Sassari, Italy

6 Department of Oncology, University of Turin Medical School, Turin, Italy

7 Department of Electrical Engineering and Electronics, University of Cagliari, Italy

Abstract

Background. Beta thalassemia major (BT) is an inherited blood disorder caused by reduced or absent synthesis of the hemoglobin beta chains, associated with profound anemia, jaundice, splenomegaly, expanded bone marrow volume, siderosis and cardiomegaly. Because of repeated blood transfusions, BT patients are subjected to peroxidative tissue injury due to secondary iron overload.
Objectives. The aim of the study was to analyze: 1) the total antioxidant capacity (TAC) value in BT patients (study group) and their healthy controls (control group) from Greece (Central Macedonia) and Italy (Sardinia); correlations between 2) the TAC and ferritin levels of BT patients, and 3) the TAC and ferritin values in BT patients with different chelation therapies;
Material and Methods. The studied group consisted of 60 subjects diagnosed with BT (41 female, mean age: 41.5 ± 9.5 years) and 40 healthy controls matched with age and sex (31 female, mean age: 38.5 ± 3.7 years). Desferrioxamine (DFO) was the basic previous chelation regimen for all BT patients. Antioxidant activity was assayed spectrophotometrically, using a TAC Kit (Total Antioxidant Capacity Colorimetric assay kit, produced by Cayman Chemical Co.), and ferritin was assayed by immunoturbidimetry.
Results. Lower levels of TAC were observed in BT patients of both countries when compared with controls (1.83 mmol/L vs 2.7 mmol/L in the Italian study group and controls and 2.42 mmol/L vs 3.2 mmol/L in the Greek study group and controls). There were no significant correlations between plasmatic TAC and ferritin. Furthermore, deferasirox was the only chelation treatment in which TAC showed a correlation in both regions.
Conclusion. Our results potentially suggest that the reduced levels of TAC detectable in BT patients could demonstrate their reduced antioxidant defensive mechanisms.

Key words

oxidative stress, β-thalassemia major, total antioxidant capacity, chelation therapies, Mediterranean countries

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