Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
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Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2017, vol. 26, nr 2, March-April, p. 207–213

doi: 10.17219/acem/31186

Publication type: original article

Language: English

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In vivo effects of curcumin and deferoxamine in experimental endometriosis

Gulnur Kizilay1,A,D, Yesim Hulya Uz1,E, Gulay Seren2,C, Enis Ulucam3,C, Ali Yilmaz3,B, Ziya Cukur4,B, Umit Ali Kayisli5,F

1 Department of Histology and Embryology, Trakya University Faculty of Medicine, Edirne, Turkey

2 Department of Analytical Chemistry, Trakya University Faculty of Pharmacy, Edirne, Turkey

3 Department of Anatomy, Trakya University Faculty of Medicine, Edirne, Turkey

4 Experimental Animal Center, Trakya University, Edirne, Turkey

5 Department of Obstetrics and Gynecology, Morsani, College of Medicine, South Florida, USA


Background. Endometriosis is one of the most common chronic gynecological diseases.
Objectives. The aim of the study was to examine the effects of curcumin and/or deferoxamine on cell proliferation in a rat model of endometriosis.
Material and Methods. Thirty female 12-week-old albino Wistar rats, weighing 200–250 g, were used in this study. All the rats underwent ovariectomy and 0.1-mg β-estradiol 17-valerate pellets were placed intraperitoneally. An experimental model of endometriosis was created in all the animals. To create the experimental model, an approximately 1-cm long section of the uterus was taken, primarily from the right horn of the uterus. Autologous fragments were then placed between the peritoneum and muscle. The animals were divided into 3 groups: Group A, treated only with the vehicle used for curcumin and deferoxamine; group B, treated with curcumin (100 mg/kg body weight); and group C, treated with deferoxamine + curcumin (100 mg/kg body weight). After biopsy samples were obtained, the sections were stained with hematoxylin and eosin. Immunostaining for cytokeratin-7 and proliferating cell nuclear antigen (PCNA) was performed. Blood iron levels were measured using a Perkin Elmer AAnalyst 800 Atomic Absorption Spectrophotometer.
Results. The endometrial implant size increased in Group A, but treatment with curcumin (p = 0.01) and deferoxamine + curcumin (p = 0.007) reduced the implant size. In ectopic endometrial epithelial cells, there were significant decreases in PCNA immunoreactivity between groups A and B (p = 0.044) and between groups A and C (p = 0.033).
Conclusion. Treatment with curcumin alone and/or in combination with deferoxamine contributed to a reduction in implant size and cell proliferation in a rat endometriosis model. Iron-chelating agents may act in the same manner when used in women with endometriosis; however, further studies from different perspectives are still needed.

Key words

curcumin, endometriosis, deferoxamine, PCNA

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