Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2017, vol. 26, nr 1, January-February, p. 155–166

doi: 10.17219/acem/43272

Publication type: review article

Language: English

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Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage

Natalia Dąbrowska1,A,B,C,D, Andrzej Wiczkowski2,E,F

1 Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK

2 Faculty of Health Sciences, The University of Bielsko-Biała, Poland


Under homeostatic conditions, an equilibrium state between amounts of free radicals formed and their scavenging is observed. Free radicals are destructive only when present in excess. Pathological changes within cells and tissues can result from a persistent excess of free radicals. Living organisms are increasingly exposed to oxidative stress, resulting in oxidative DNA modifications. One such modification is 8-hydroxy-2’-deoxyguanosine (8-OHdG). It is considered a biomarker of oxidative stress and oxidative DNA damage. It has been found both in physiological fluids and in cells. This paper presents methods found in the literature for determining 8-OHdG expression in various kinds of biological material – blood, urine or liver homogenates. Methods for determining the biomarker expression have been grouped into direct and indirect methods, and the various levels of 8-hydroxy-2’-deoxyguanosine that can be determined by the different techniques are presented. The basic pros and cons of the various techniques are also discussed.

Key words

ELISA, free radicals, HPLC, 8-hydroxy-2’-deoxyguanosine, 32P-postlabeling

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