Advances in Clinical and Experimental Medicine
2017, vol. 26, nr 1, January-February, p. 155–166
Publication type: review article
Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage
1 Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK
2 Faculty of Health Sciences, The University of Bielsko-Biała, Poland
Under homeostatic conditions, an equilibrium state between amounts of free radicals formed and their scavenging is observed. Free radicals are destructive only when present in excess. Pathological changes within cells and tissues can result from a persistent excess of free radicals. Living organisms are increasingly exposed to oxidative stress, resulting in oxidative DNA modifications. One such modification is 8-hydroxy-2’-deoxyguanosine (8-OHdG). It is considered a biomarker of oxidative stress and oxidative DNA damage. It has been found both in physiological fluids and in cells. This paper presents methods found in the literature for determining 8-OHdG expression in various kinds of biological material – blood, urine or liver homogenates. Methods for determining the biomarker expression have been grouped into direct and indirect methods, and the various levels of 8-hydroxy-2’-deoxyguanosine that can be determined by the different techniques are presented. The basic pros and cons of the various techniques are also discussed.
ELISA, free radicals, HPLC, 8-hydroxy-2’-deoxyguanosine, 32P-postlabeling
- Czajka A. Wolne rodniki tlenowe a mechanizmy obronne organizmu. Nowiny Lekarskie. 2006;76(5):582–586.
- Kulbacka J, SJ, Chwilkowska A. Stres oksydacyjny w procesach uszkodzenia komórek. Pol Merkuriusz Lek. 2009;XXVII(157):44–47.
- Hu Ch-W, CMR. Assessment of oxidative stress using urinary 8-oxodG and 8-oxo-Gua levels. Clinical Labolatory International. 2010;34:12–14.
- Bailey SM, Landar A, Darley-Usmar V. Mitochondrial proteomics in free radical research. Free Radic Biol Med. 2005;38(2):175–188.
- Sakharov DV et al. Prolonged lipid oxidation after photodynamic treatment. Study with oxidation-sensitive probe C11-BODIPY581/591. FEBS Letters. 2005;579(5):1255–1260.
- Niedernhofer LJ, et al. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem. 2003;278(33):31426–31433.
- Klaunig JE, Wang Z, Pu X, Shou S. Oxidative stress and oxidative damage in carcinogenesis. Toxicol Pathol. 2010;38(1):96–109.
- Ponczek MB, WB. Oddziaływanie reaktywnych form tlenu i azotu z białkami. Postępy Biochemii. 2005;51:140–145.
- Balcerczyk A, Bartosz G. Thiols are main determinants of total antioxidant capacity of cellular homogenates. Free Radic Res. 2003;37(5):537–541.
- Dalle-Donne I, et al. Protein carbonylation, cellular dysfunction, and disease progression. J Cell Mol Med. 2006;10(2):389–406.
- Kawanishi S, Hiraku Y, Oikawa S. Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging. Mutat Res. 2001;488(1):65–76.
- Sastry PS, Rao KS. Apoptosis and the nervous system. J Neurochem. 2000;74(1):1–20.
- Rahman T, Hosen I, Islam MMT, Shekhar HU. Oxidative stress and oxidative damage in carcinogenesis. Adv Biosci Biotechnol. 2012;3:997–1019.
- Hallmann A. Ocena cytotoksycznego działania menadionu, wodoronadtlenku tert-butylu i nadtlenku wodoru w komórkach Choriocarcino-ma, praca doktorska, Gdańsk, 2008.
- Kryston TB, Georgiev AB, Pissis P. Role of oxidative stress and DNA damage in human carcinogenesis. Mutation Research. 2011;711:193–201.
- Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2’ -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009;27(2):120–139.
- Hajas G, et al. Biochemical identification of a hydroperoxide derivative of the free 8-oxo-7,8-dihydroguanine base. Free Radic Biol Med. 2012;52:749–756.
- Morikawa M, et al., Analysis of guanine oxidation products in double-stranded DNA and proposed guanine oxidation pathways in sin-gle-stranded, double-stranded or quadruplex DNA. Biomolecules. 2014;4(1):140–159.
- Nicotera TM, Munson BR, Fiel RJ. Photosensitized formation of 8-hydroxy-2’-deoxyguanosine and DNA strand breakage by a cationic me-so-substituted porphirin. Photochem Photobiol. 1994;60(4):295–300.
- Floyd RA, et al. Serendipitous findings while researching oxygen free radicals. Free Radic Biol Med. 2009;46:1004–1013.
- Martinez GR, et al. Identification of the main oxidation products of 8-methoxy-2’-deoxyguanosine by singlet molecular oxygen. Free Radic Biol Med. 2005;38(11):1491–1500.
- Schneider JE, et al. Methylene blue plus light mediates 8-hydroxy 2’-deoxyguanosine formation in DNA preferentially over strand breakage. Nucleic Acids Res. 1990;18(3):631–635.
- Schneider JE Jr., et al. Methylene blue and rose bengal photoinactivation of RNA bacteriophages: Comparative studies of 8-oxoguanine formation in isolated RNA. Arch Biochem Biophys. 1993;301(1):91–97.
- Nilov DI, et al. Oxidation of adenosine and inosine: The chemistry of 8-oxo-7,8-dihydropurines, purine iminoquinones, and purine quinones as observed by ultrafast spectroscopy. J Am Chem Soc. 2013;135(9):3423–3438.
- Roszkowski K, et al. Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer. Med Sci Monit. 2011;17(6):329–333.
- Bialkowski K. Metabolizm pochodnych 8-oksyguaniny. Praca doktorska, Bydgoszcz, 1997.
- Brack M, et al. Distinct profiles of systemic biomarkers of oxidative stress in chronic human pathologies: Cardiovascular, psychiatric, neuro-degenerative, rheumatic, infectious, neoplasmic and endocrinological diseases. Adv Biosci Biotechnol. 2013;4:331–339.
- Kasai H. Chemistry-based studies on oxidative DNA damage: Formation, repair, and mutagenesis. Free Radic Biol Med. 2002;33(4): 450–456.
- Gannett PM. Base pairing of 8-oxoguanosine and 8-oxo-2’-deoxyguanosine with 2’-deoxycytidine, 2’-deoxyguanosine and thymidine. Chem-ical Research in Toxicology. 1993;6:690–700.
- Hamm ML, et al. Biochemical investigations into the mutagenic potential of 8-Oxo-2’-deoxyguanosine using nucleotide analogues. Chem Res Toxicol. 2012;25(11):2577–2588.
- Plum GE, et al. Influence of the oxidatively damaged adduct 8-oxodeoxyguanosine on the conformation, energetics, and thermodynamic stability of a DNA duplex. Biochemistry. 1995;34(49):16148–16160.
- Kasai H, Nishimura S. Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids Res. 1984;12(4):2137–2145.
- Cheng K, et al. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G----T and A----C substitutions. J Biol Chem. 1992;267:166–172.
- Valko M, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44–84.
- Bolner A. Plasma and urinary HPLC-ED determination of the ratio of 8-OHdG/2-dG in Parkinson’s disease. Clin Lab. 2011;57(11–12):859–866.
- Wu LL, et al. Urinary 8-OHdG: A marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2004;339(1–2):1–9.
- Lunec J, et al. 8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus. FEBS Lett. 1994;348(2):131–138.
- Gmitterova K, et al. 8-OHdG in cerebrospinal fluid as a marker of oxidative stress in various neurodegenerative diseases. Neurodegener Dis. 2009;6(5–6):263–269.
- Domijan AM, Peraica M. Determination of 8-hydroxy-2’deoxyguanosine in urine using HPLC with electrochemical detection. Arh Hig Rada Toksikol. 2008;59(4):277–282.
- De Martinis BS, de Lourdes Pires Bianchi M. Methodology for urinary 8-hydroxy-2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res. 2002;46(2):129–131.
- Matayatsuk C, Wilairat P. Quantitative determination of 8-hydroxy-2’-deoxyguanosine as a biomarker of oxidative stress in thalassemic pa-tients using HPLC with an electrochemical detector. Journal of Analytical Chemistry. 2008;63(1):52–56.
- Kasai H, Kawai K, Li YS. Analysis of 8-OH-dG and 8-OH-Gua as biomarkers of oxidative stress. Genes and Environment. 2008;30(2):33–40.
- Shimoi K, et al. Comparison between high-performance liquid chromatography and enzyme-linked immunosorbent assay for the determi-nation of 8-hydroxy-2’-deoxyguanosine in human urine. Cancer Epidemiol Biomarkers Prev. 2002;11(8):767–770.
- Gedik CM, Wood SG, Collins AR. Measuring oxidative damage to DNA;HPLC and the comet assay compared. Free Radic Res. 1998;29(6):609–615.
- Nicotera T, Bardin S. Electrochemical detection of 8-hydroxy-2-deoxyguanosine levels in cellular DNA. In: Armstrong D, ed. Free radical and antioxidant protocols. Humana Press 1998:181–190.
- Foksinski M, et al. Evaluation of 8-oxodeoxyguanosine, typical oxidative DNA damage, in lymphocytes of ozone-treated arteriosclerotic patients. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 1999;438(1):23–27.
- Takeuchi T, et al. Evaluation of 8-hydroxydeoxyguanosine, a typical oxidative DNA damage, in human leukocytes. Carcinogenesis. 1994;15(8):1519–1523.
- Park JW, Cundy KC, Ames BN. Detection of DNA adducts by high-performance liquid chromatography with electrochemical detection. Car-cinogenesis. 1989;10(5):827–832.
- Inaba Y, et al. Development of urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) measurement method combined with SPE. J Chromatogr Sci. 2011;49(4):303–309.
- Lengger CG, Schoch, Topp H. A high-performance liquid chromatographic method for the determination of 8-oxo-7,8-dihydro-2’-deoxyguanosine in urine from man and rat. Anal Biochem. 2000;287(1):65–72.
- Evans MD, et al. Analysis of urinary 8-oxo-7,8-dihydro-purine-2’-deoxyribonucleosides by LC-MS/MS and improved ELISA. Free Radic Res. 2008;42(10):831–840.
- Shi M, et al. Generation of 8-hydroxydeoxyguanosine from DNA using rat liver homogenates. Cancer Sci. 2005. 96(1):13–18.
- Dizdaroglu M. Application of capillary gas chromatography-mass spectrometry to chemical characterization of radiation-induced base damage of DNA: Implications for assessing DNA repair processes. Anal Biochem. 1985;144(2):593–603.
- Dizdaroglu M. Gas chromatography-mass spectrometry of free radical-induced products of pyrimidines and purines in DNA. Methods Enzy-mol. 1990;193:842–857.
- Fraga CG. Ascorbic acids protects against endogenous oxidative DNA damage in human sperm. Proc Natl Acad Sci U S A. 1991;88:11003–11006.
- Malayappan B, et al. Urinary analysis of 8-oxoguanine, 8-oxoguanosine, fapy-guanine and 8-oxo-2’-deoxyguanosine by high-performance liquid chromatography-electrospray tandem mass spectrometry as a measure of oxidative stress. J Chromatogr A. 2007;1167(1):54–62.
- Nackerdien Z, Olinski R, Dizdaroglu M. DNA base damage in chromatin of gamma-irradiated cultured human cells. Free Radic Res Commun. 1992;16(4):259–273.
- Gupta RC, Arif JM. An improved (32)P-postlabeling assay for the sensitive detection of 8-oxodeoxyguanosine in tissue DNA. Chem Res Toxi-col. 2001;14(8):951–957.
- Jones NJ. ³²P-postlabelling for the sensitive detection of DNA adducts. Methods Mol Biol. 2012;817:183–206.
- Ravanat JL. Measuring oxidized DNA lesion as biomarkers of oxidative stress: An analytical challenge. FABAD Journal of Pharmaceutical Science. 2005;30:100–113.
- Lequin RM. Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). Clinical Chemistry. 2005;51(12):2415–2418.
- Yin B, et al. Determination of 8-hydroxydeoxyguanosine by an immunoaffinity chromatography-monoclonal antibody-based ELISA. Free Radic Biol Med. 1995;18(6):1023–1032.
- Ide H, et al. Antibodies to oxidative DNA damage: Characterization of antibodies to 8-oxopurines. Cell Biol Toxicol. 1997;13(6):405–417.
- Khalil AM, AAM, Gagaa MH, Detection of oxidative stress induced by mobile phone radiation in tissues of mice using 8-oxo-7,8-dihydro-2’-deoxyguanosine as a biomarker. WASET. 2011;52:657–662.
- Haghdoost S, et al. Extracellular 8-oxo-dG as a sensitive parameter for oxidative stress in vivo and in vitro. Free Radic Res. 2005;39(2):153–162.
- Cooke MS, et al. Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7,8-dihydro-2’-deoxyguanosine in saliva and urine. Free Radic Biol Med. 2006;41(12):1829–1836.
- ESCODD, Measurement of DNA oxidation in human cells by chromatographic and enzymic methods. Free Radic Biol Med. 2003;34:1089–1099.
- Collins AR, Dusinska M. Oxidation of cellular DNA measured with the comet assay. Methods Mol Biol. 2002;186:147–159.
- Germadnik DA, Pilger, Rudiger HW. Assay for the determination of urinary 8-hydroxy-2’-deoxyguanosine by high-performance liquid chro-matography with electrochemical detection. J Chromatogr B Biomed Sci Appl. 1997;689(2):399–403.
- Shigenaga MK, Gimeno CJ, Ames BN. Urinary 8-hydroxy-2’-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. Proc Natl Acad Sci U S A. 1989;86(24):9697–9701.
- Pilger A, et al. Urinary excretion of 8-hydroxy-2’-deoxyguanosine measured by high-performance liquid chromatography with electro-chemical detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;778(1–2):393–401.
- Lagadu S, et al. 8-oxo-7,8-dihydro-2’-deoxyguanosine as a biomarker of oxidative damage in oesophageal cancer patients: Lack of association with antioxidant vitamins and polymorphism of hOGG1 and GST. Journal of Experimental & Clinical Cancer Research. 2010;29(1):157.
- Schins RP, Schilderman PA, Borm PJ. Oxidative DNA damage in peripheral blood lymphocytes of coal workers. Int Arch Occup Environ Health. 1995;67(3):153–157.
- Hofer T, Moller L. Optimization of the workup procedure for the analysis of 8-oxo-7,8-dihydro-2’-deoxyguanosine with electrochemical detection. Chem Res Toxicol. 2002;15(3):426–432.
- Marczynski B, et al. Levels of 8-hydroxy-2’-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany. Mutat Res. 2000;468(2):195–202.
- Lodovici M, et al. Oxidative liver DNA damage in rats treated with pesticide mixtures. Toxicology. 1997;117(1):55–60.
- Hamilton ML, et al. A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using sodium ioide method to isolate DNA. Nucleic Acids Res. 2001;29(10):2117–2126.