Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 6, November-December, p. 1293–1301

doi: 10.17219/acem/63795

Publication type: original article

Language: English

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Using an Electron Scanning Microscope to Assess the Penetrating Abilities of an Experimental Preparation with Features of a Dental Infiltrant: Preliminary Study

Małgorzata Skucha-Nowak1,A,B,C,D,E,F, Anna Mertas2,B, Marta Tanasiewicz1,E

1 Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland

2 Chair and Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Zabrze, Poland


Background. The resin infiltration technique is one of the micro-invasive methods whose aim is the penetration of demineralized enamel with a low viscosity resin. This technique allows the dentist to avoid the application of mechanical means of treatment.
Objectives. The objective of this preliminary study was to attempt to determine the possibilities of using an electron microscope to assess the penetrating abilities of an experimental preparation with features of a dental infiltrant and to compare the depth of infiltration of the designed experimental preparation with an infiltrant available on the market.
Material and Methods. A bioactive methacrylate monomer based on PMMAn with built-in metronidazole was synthesized. The commercially available Icon solution (with contrast agent YbF3) and the experimental solution were applied to the relevant parts of teeth. The dissected sections along the long tooth axis and polished surfaces were then examined with use of an electron scanning microscope.
Results. The backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small YbF3 particles.
Conclusion. The authors concluded that the backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small particles of the contrast agent. In order to prevent blockage of decalcified enamel tissue by ytterbium trifluoride (YbF3) grains, a nanoparticle form of that compound should be used (that is, particles with sizes in the range of 10–9 m).

Key words

microinvasive dentistry, ytterbium trifluoride, electron scanning microscope, experimental infiltrants

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