Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 6, November-December, p. 1293–1301

doi: 10.17219/acem/63795

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Using an Electron Scanning Microscope to Assess the Penetrating Abilities of an Experimental Preparation with Features of a Dental Infiltrant: Preliminary Study

Małgorzata Skucha-Nowak1,A,B,C,D,E,F, Anna Mertas2,B, Marta Tanasiewicz1,E

1 Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland

2 Chair and Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Zabrze, Poland

Abstract

Background. The resin infiltration technique is one of the micro-invasive methods whose aim is the penetration of demineralized enamel with a low viscosity resin. This technique allows the dentist to avoid the application of mechanical means of treatment.
Objectives. The objective of this preliminary study was to attempt to determine the possibilities of using an electron microscope to assess the penetrating abilities of an experimental preparation with features of a dental infiltrant and to compare the depth of infiltration of the designed experimental preparation with an infiltrant available on the market.
Material and Methods. A bioactive methacrylate monomer based on PMMAn with built-in metronidazole was synthesized. The commercially available Icon solution (with contrast agent YbF3) and the experimental solution were applied to the relevant parts of teeth. The dissected sections along the long tooth axis and polished surfaces were then examined with use of an electron scanning microscope.
Results. The backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small YbF3 particles.
Conclusion. The authors concluded that the backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small particles of the contrast agent. In order to prevent blockage of decalcified enamel tissue by ytterbium trifluoride (YbF3) grains, a nanoparticle form of that compound should be used (that is, particles with sizes in the range of 10–9 m).

Key words

microinvasive dentistry, ytterbium trifluoride, electron scanning microscope, experimental infiltrants

References (15)

  1. Skucha-Nowak M, Tanasiewicz M, Gibas M, Twardawa H: Barriers securing the patient’s own tissues against influence of the oral cavity environment than can be used in low invasive dentistry. Pol J Environ Stud 2012, 21, 25–29.
  2. Subramaniam P, Girish Babu KL, Lakhotia D: Evaluation of penetration depth of a commercially available resin infiltrate into artificially created enamel lesions: An in vitro study. J Conserv Dent 2014, 17, 146–149.
  3. Kmieć Z: Powstawanie i właściwości szkliwa. In: Histologia i cytofizjologia zęba i jamy ustnej. Eds: Stoces I, Sokołowska-Trelka A. Wydawnictwo Elsevier Urban & Partner, wyd. I, Wrocław, Poland, 2007, 34–52.
  4. Skucha-Nowak M, Gibas M, Tanasiewicz M, Twardawa H, Szklarski T: Natural and controlled demineralization for study purposes in minimally invasive dentistry. Adv Clin Exp Med 2015, 24, 891–898.
  5. Kielbassa AM, Műller A, Gerhard CR: Closing the gap between oral hygiene and minimally invasive infiltration technique of incipient (proximal) enamel lesions. Quintessence Int 2009, 40, 663–681.
  6. Bronk M, Samet A, Dąbrowski K: Metronidazol–lek, który nie utracił pierwotnej aktywności przeciwbakteryjnej. Zakażenia 2010, 10, 35–38 [in Polish].
  7. Kasten M: Repetytorium z leków przeciwdrobnoustrojowych cz. VIII – klindamycyna, metronidazol i chloramfenikol. Mayo Clin Proc 1999, 74, 825–833.
  8. Skucha-Nowak M, Tanasiewicz M, Gibas M, Twardawa H: Analysis of the composition of preparations used as a barrier to protect tissues of the patient against the influence of the environment in the oral cavity. Pol J Environ Stud 2013, 22, 53–57.
  9. Skucha-Nowak M: Attempt to assess the infiltration of enamel made with experimental preparation using a scanning electron microscope. Open Med. Cent Eur J Med 2015, 10, 238–248.
  10. Yang F, Mueller J, Kielbassa AM: Surface substance loss of subsurface bovine enamel lesions after different steps of the resinous infiltration technique: A 3D topography analysis. Odontology 2012, 100, 172–180.
  11. Paris S, Lausch J, Selje T, Dorfer CE, Meyer-Luckel H: Comparison of sealant and infiltrant penetration into pit and fissure caries lesions in vitro. J Dent 2014, 42, 432–438.
  12. Paris S, Soviero VM, Chatzidakis AJ, Meyer-Lueckel H: Penetration of experimental infiltrants with different penetration coefficients and ethanol addition into natural caries lesions in primary molars. Caries Res 2012, 46, 113–117.
  13. Nowak M, Kotyczka-Morańska M, Szperlich P, Bober Ł, Jesionek M, Kępińska M, Stróż D, Kusz J, Szala J, Moskal G, Rzychoń T, Młyńczak J, Kopczyński K: Using of sonochemically prepared components for vapor phase growing of SbI3•3S8. Ultrasonics Sonochem 2010, 17, 892–901.
  14. Prentice LH, Tyas MJ, Burrow MF: The effect of ytterbium fluoride and barium sulphate nanoparticles on the reactivity and strength of a glass-ionomer cement. Dental Mater 2006, 22, 746–751.
  15. Kupka T, Tanasiewicz M, Gibas M, Dąbrowska A: Porównawcze badania laboratoryjne eksperymentalnego uszczelniacza dołków i bruzd zębowych SPBS (Smart Polymer – based Bio-Sealant). Czas Stomatol 2006, 59, 191–196 [in Polish].
© Wroclaw Medical University