Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 4, July-August, p. 673–679

doi: 10.17219/acem/62922

Publication type: original article

Language: English

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Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms

Magdalena Górnicka1,A,B,C,D,F, Małgorzata Drywień1,B,C,F, Joanna Frąckiewicz1,B,F, Bogdan Dębski2,E,F, Agata Wawrzyniak1,E,F

1 Department of Human Nutrition, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Poland

2 Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland


Background. Training-induced oxidative stress can be reduced by α-tocopherol. Adequate intake of α-tocopherol could have health benefits for previously untrained young subjects.
Objectives. The aim of this study was to determine the effects of training and different doses of α-tocopherol on exercise-induced oxidative stress in rat livers.
Material and Methods. Young male Wistar rats (n = 40) were randomly divided into eight groups (undergoing training and not undergoing training, given orally administered α-tocopherol doses of 0, 0.5, 1.0 and 4.0 mg). Every day for 10 consecutive days, the rats in the training groups ran for 15 min on a treadmill at 20 m/min to induce oxidative stress. Hepatic oxidative stress was evaluated based on the liver concentrations of 8-hydroxy-2’-deoxyguanosine (8-OHdG) and thiobarbituric acid reactive substances (TBARS).
Results. The liver concentrations of α-tocopherol were significantly influenced by α-tocopherol doses (p < 0.001) and physical exercise (p < 0.001). The liver concentrations of α-tocopherol increased in response to the highest dose (4 mg/d) of α-tocopherol in the non-training groups. In the training groups, the liver concentrations of α-tocopherol were independent of the dose. The levels of TBARS, a marker of lipid peroxidation, were lowest in the training and non-training rats administered 4.0 mg of α-tocopherol. Physical exercise and α-tocopherol doses significantly influenced TBARS concentrations (p = 0.004, p < 0.05).
Conclusion. The results of this study indicate that running training causes lipids peroxidation and reduces α-tocopherol levels in the liver, but it does not contribute to DNA damage. Increased liver concentrations of α-tocopherol were found to exert a protective effect against oxidative damage induced by endurance training. An adequate intake of α-tocopherol is important for previously untrained young subjects.

Key words

oxidative stress, rats, 8-OHdG, α-tocopherol, TBARS

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