Advances in Clinical and Experimental Medicine
2016, vol. 25, nr 4, July-August, p. 611–615
Publication type: original article
The Formation of Blood Vessel After the Administration of the Plasmid Encoding Ang-1 Gene in Fischer Rats
1 Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland
2 Department of Cardiac Surgery, Wroclaw Medical University, Poland
3 Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Poland
4 Wrovasc – Integrated Cardiovascular Centre, Regional Specialist Hospital in Wrocław, Research and Development Centre, Poland
5 Department of Pediatric BMT, Oncology and Hematology, Wroclaw Medical University, Poland
6 Department of Anaesthesiology and Intensive Therapy, Wroclaw Medical University, Poland
7 Department of Pathology, Wroclaw Medical University, Poland
Background. Chronic limb ischemia is a serious clinical problem. Patients who do not qualify for standard treatment may benefit from novel gene therapies.
Objectives. This study evaluated angiogenesis following intramuscular injections of angiogenic plasmid Ang-1 in Fisher rats.
Material and Methods. Twenty rats had plasmids injected intramuscularly in their hind limbs. The study group consisted of 10 animals which received the Ang-1 plasmid, while the control group consisted of 10 rats that received an empty plasmid. All the animals were euthanized after 12 weeks and tissue samples from the hind limb thigh muscles and internal organs were harvested for histological and immunohistochemical examinations. To assess the angiogenesis the number of vessels in the hind limb muscles visualized by the SMA and FVIII markers was counted for each animal in five separate microscopic fields.
Results. There were no pathological lesions or any signs of neoplastic angiogenesis in any of the 20 rats. The number of vessels visualized by the FVIII marker in the study group was two times higher than in the control group (median: 12, range: 7–25 vs. median: 6, range: 2–15; p < 0.0001). The median estimated that the number of vessels visualized by the SMA marker is 63% higher in the study group compared to the control group (median: 6.5, range: 1–12 vs. median: 4, range: 0–10; p = 0.0008).
Conclusion. Intramuscular injections of Ang-1 plasmids induced angiogenesis in the rat hind limb muscles.
angiogenesis, lower limb ischemia, rats, proangiogenic factors
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