Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
Scopus CiteScore – 3.4 (CiteScore Tracker 3.4)
Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 3, May-June, p. 415–423

doi: 10.17219/acem/38786

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

The Effect of Silver Carp Skin-Derived Peptides on the Activities of VEGF and Hsp70.1 Gene Transcription Promoters

Diana Wojtkowiak1,B,D, Janina Piechowicz1,B,C, Jolanta Grzenkowicz-Wydra2,B, Stanisław Wosiński3,B,D, Marzena Dominiak4,E,F, Jakub Hadzik4,D, Andrzej F. Frydrychowski1,A,C,F

1 Institute of Human Physiology, Faculty of Health Sciences, Medical University of Gdańsk, Poland

2 Biotechnological Laboratory, Pomeranian Science and Technology Park, Gdynia Innovation Center, Poland

3 Laboratory of EPR Tomography, Institute of Materials Technology, Poznan University of Technology, Poland

4 Department of Dental Surgery, Wroclaw Medical University, Poland


Background. Studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently attempts are being made to replace animal collagen with fish collagen.
Objectives. The aim of the study was to compare the effect of silver carp skin-derived peptide extract on the transcriptional activities of human VEGF and hsp70.1 gene promoters inserted into the plasmids with secreted alkaline phosphatase as a reporter gene.
Material and Methods. Changes in the activity of the promoters were investigated using a HEK293FT cell line transfected with pVEGF-SEAP or pHsp70-SEAP. The cells were cultured in dishes containing peptides separated using reverse-phase high performance liquid chromatography.
Results. The study demonstrated that the silver carp skin-derived peptide extract exerts both an inhibitory effect on the VEGF gene promoter and activating effect on the hsp70.1 gene promoter. Higher biological activity was recorded in the case of a freshly prepared peptide extract compared to one stored at 4°C for three months.
Conclusion. The silver carp skin-derived collagen peptides influence VEGF and hsp70.1 gene promoters’ transcriptional activity.

Key words

collagen, fish collagen, skin-derived peptide, gene promoters

References (29)

  1. Jongjareonrak A, Benjakul S, Visessanguan W, Nagai T, Tanaka M: Isolation and characterization of acid and pepsinsolubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta). Food Chemistry 2005, 93, 475–262.
  2. Tran KT, Griffith L, Wells A: Extracellular matrix signaling through growth factor receptors during wound healing. Wound Repair Regen 2004, 12, 262–268.
  3. Maquart FX, Pasco S, Ramont L, Hornebeck W, Monboisse JC: An introduction to matrikines: Extracellular matrix-derived peptides which regulate cell activity. Implication in tumor invasion. Crit Rev Oncol Hematol 2004, 49, 199–202.
  4. Desoize B: Stromal reaction and tumour growth. Crit Rev Oncol Hematol 2004, 49, 173–176.
  5. Chai HJ, Li JH, Huang HN, Li TL, Chan YL, Shiau CY, Wu CJ: Effects of sizes and conformations of fish-scale collagen peptides on facial skin qualities and transdermal penetration efficiency. J Biomed Biotechnol 2010, 2010:757301. DOI: 10.1155/2010/757301. Epub 2010 Jun 8.
  6. Maquart FX, Bellon G, Pasco S, Monboisse JC: Matrikines in the regulation of extracellular matrix degradation. Biochimie 2005, 87, 353–360.
  7. Fujioka K, Maeda M, Hojo T, Sano A: Protein release from collagen matrices. Adv Drug Deliv Rev 1998, 31, 247–266.
  8. Ahmed MR, Venkateshwarlu U, Jayakumar R: Multilayered peptide incorporated collagen tubules for peripheral nerve repair. Biomaterials 2004, 25, 2585–2594.
  9. Olsen D, Yang CL, Bodo M, Chang R, Leigh S, Baez J, Carmichael D, Perala M, Hamalainen ER, Jarvinen M: Recombinant collagen and gelatin for drug delivery. Adv Drug Deliv Rev 2003, 55, 1547–1567. DOI: 10.1016/j. addr.2003.08.008.
  10. Wojtkowiak D, Frydrychowski AF, Hadzik J, Dominiak M: Identification of Small Peptides of Acidic Collagen Extracts from Silver Carp Skin and Their Therapeutic Relevance. Adv Clin Exp Med 2016, 25, 2, 227–235.
  11. Xie K, Wei D, Shi Q, Huang S: Constitutive and inducible expression and regulation of vascular endothelial growth factor. Cytokine Growth Factor Rev 2004, 15, 297–324.
  12. Toi M, Bando H, Weich HA: Vascular endothelial growth factor and its relationships with endogenous inhibitors in a breast cancer microenvironment manipulated by hormonal therapy: A hypothetical consideration. Biomed Pharmacother 2005, 59, Suppl 2, 344–347.
  13. Liekens S, De Clercq E, Neyts J: Angiogenesis: Regulators and clinical applications. Biochem Pharmacol 2001, 61, 253–270.
  14. Dvorak H: Angiogenesis: Update 2005. J Thromb Haemost 2005, 3, 1835–1842.
  15. Loureiro RMB, D’Amore PA: Transcriptional regulation of vascular endothelial growth factor in cancer. Cytokine Growth Factor Rev 2005, 16, 77–89.
  16. Luo J, Xiong Y, Han X, Lu Y: VEGF non-angiogenic functions in adult organ homeostasis: Therapeutic implications. J Mol Med (Berl) 2011, 89, 635–645. DOI: 10.1007/s00109-011-0739-1. Epub 2011 Mar 2.
  17. Eichmann A, Simons M: VEGF signaling inside vascular endothelial cells and beyond. Curr Opin Cell Biol 2012, 24, 188–193. DOI: 10.1016/ Epub 2012 Feb 25.
  18. Cao Y: Endogenous angiogenesis inhibitors and their therapeutic implications. Int J Biochem Cell Biol 2001, 33, 357–369.
  19. Asea A, Rehli M, Kabingu E, Boch JA, Bare O, Auron PE, Stevenson MA, Calderwood SK: Novel signal transduction pathway utilized by extracellular HSP70: Role of TLR2 and TLR4. J Biol Chem 2002, 277, 15028–15034. Epub 2002 Feb 8.
  20. Thériault JR, Mambula SS, Sawamura T, Stevenson MA, Calderwood SK: Extracellular HSP70 binding to surface receptors present on antigen presenting cells and endothelial/epithelial cells. FEBS Lett 2005, 579, 1951–1960.
  21. Laplante AF, Moulin V, Auger FA, Landry J, Li H, Morrow G, Tanguay RM, Germain L: Expression of heat shock proteins in mouse skin during wound healing. J Histochem Cytochem 1998, 46, 1291–1301.
  22. Oberringer M, Baum HP, Jung V, Welter C, Frank J, Kuhlmann M, Mutschler W, Hanselmann RG: Differential expression of heat shock protein 70 in well healing and chronic human wound tissue. Biochem Biophys Res Commun 1995, 214, 1009–1014.
  23. Helbig D, Bodendorf M, Anderegg U, Simon JC, Paasch U: A human skin explant model to study molecular changes in response to fractional photothermolysis: Spatio-temporal expression of HSP70, Medical Laser Application 01/2010; 25, 173–180. DOI: 10.1016/j.mla.2009.12.002.
  24. Mushtaq S, Naqvi ZA, Siddiqui AA, Ahmed N: Albumin precursor and Hsp70 modulate corneal wound healing in an organ culture model. Acta Histochem 2011, 113, 36–42. DOI: 10.1016/j.acthis.2009.07.009. Epub 2009 Sep 9.
  25. Ko JA, Yanai R, Quan WY, Morishige N, Nishida T: Up-regulation of HSP-70 by the fibronectin-derived peptide PHSRN in human corneal epithelial cells. Biochem Biophys Res Commun 2008, 370, 424–428. DOI: 10.1016/j. bbrc.2008.03.093. Epub 2008 Mar 27.
  26. Yao Y, Zhang G, Zhang Y, Li W, Wang C, Yin C, Zhang F: Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HS70 and inhibition of NF-κB. Cell Stress Chaperones 2011, 16, 287– 296. DOI: 10.1007/s12192-010-0242-6
  27. Dokladny K, Lobb R, Wharton W, Ma TY, Moseley PL: LPS-induced cytokine levels are repressed by elevated expression of HSP70 in rats: Possible role of NF-κB, Cell Stress Chaperones 2010, 15, 153–163. DOI: 10.1007/ s12192-009-0129-6. Epub 2009 Jun 24.
  28. Dulak J, Tomala K, Loboda A, Józkowicz A: Nitric oxide-dependent synthesis of vascular endothelial growth factor is impaired by high glucose. Life Sci 2004, 75, 2573–2586.
  29. Grzenkowicz-Wydra J, Cisowski J, Nakonieczna J, Zarebski A, Udilova N, Nohl H, Józkowicz A, Podhajska A, Dulak J: Gene transfer of CuZn superoxide dismutase enhances the synthesis of vascular endothelial growth factor. Mol Cell Biochem 2004, 264, 169–181.