Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 5, September-October, p. 891–898

doi: 10.17219/acem/28903

Publication type: review

Language: English

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Natural and Controlled Demineralization for Study Purposes in Minimally Invasive Dentistry

Małgorzata Skucha-Nowak1,A,B,C,D,F, Mirosław Gibas2,B,C,E, Marta Tanasiewicz1,A,E,F, Henryk Twardawa3,B, Tomasz Szklarski3,B

1 Department of Conservative Dentistry with Endodontics, Medical University of Silesia, Poland

2 Department of Organic, Bio-Organic Chemistry and Biotechnology, Technical University of Silesia in Gliwice, Poland

3 Department of Preventive Dentistry and Endodontics, Medical University of Silesia in Katowice, Poland

Abstract

Artificially induced demineralization of enamel is frequently used during laboratory tests, particularly in minimally invasive dentistry. The aim of this study was to analyze demineralization techniques of hard tooth tissue applicable in the research of materials in minimally invasive dentistry. The most important factor taken into consideration when designing a method is to make a model as closely similar to the natural environment of the human oral cavity as it is possible. In vitro models allow us to maintain stability and control over the environment and guarantee repeatability of the results. There are main models to produce dental caries outside of the body. The first model is chemical and it uses acids. It is simplified and reflects the actual environment of the oral cavity to a lesser degree. The second model is biological and it is more accurate as it uses microorganisms which build the dental plaque. Among in vitro protocols are also used pH-cycling models. Based on the available literature, it was found that bovine teeth and human teeth with demineralization diagnosed while still inside the oral cavity are the most frequently used kind of specimens in research conducted with use of the chemical model. Not a single case of use of the biological and pH-cycling models were found in the available literature related to the research of infiltrants in minimally invasive dentistry.

Key words

enamel, demineralization, dentistry

References (26)

  1. Gębska A: Uzyskiwanie wczesnych postaci próchnicy szkliwa zębów ludzkich metodami in vitro. Praca doktorska, Pomorska Biblioteka Cyfrowa, Gdańsk 2009.
  2. Wójtowicz A, Malm A: Mikrobiologiczne podłoże próchnicy w aspekcie jej profilaktyki. Farm Pol 2009, 65, 327–330.
  3. Fejerskov O, Kidd E: Próchnica zębów. Choroba próchnicowa i postępowanie kliniczne. Wydanie I polskie pod red. Kaczmarek U. Urban & Partner, Wrocław 2006, 132–186.
  4. Krol DM: Dental caries, oral health, and pediatricians. Curr Probl Pediatr Adolesc Health Care 2003, 33, 253–270.
  5. Jańczuk Z: Stomatologia zachowawcza. Wyd Lek PZWL, Warszawa 2008, 63–81.
  6. Cameron AC, Widmer RP: Stomatologia dziecięca. Wydanie II polskie pod red. Kaczmarek U. Urban & Partner, Wrocław 2012, 188–237.
  7. He LH, Swain MV: Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics. J Mech Behav Biomed Mater 2008, 1, 18–29.
  8. Sierpińska T, Stocka A, Gołębiewska M: Struktura szkliwa, właściwości śliny oraz bruksizm jako czynniki etiologiczne odpowiedzialne za starcie patologiczne. Przegląd piśmiennictwa. Protet Stomatol 2008, 58, 100–104.
  9. Kaczmarek U: Mechanizmy kariostatyczne fluoru. Czas Stom 2005, 58, 404–413.
  10. Ilewicz L, Skucha-Nowak M, Renk S: Zastosowanie chemo-mechanicznego systemu „Carisolv” w usuwaniu próchnicowo zmienionej zębiny. Twój Mag Med – Stomatologia i Protetyka 2002, 7, 30–38.
  11. Lewandowska KD: Czy subdyfuzja warunkuje rozwój próchnicy szkliwa? Ann Acad Med Gedan 2005, 35, 133–138.
  12. Piesiak-Pańczyszyn D, Czajczyńska-Waszkiewicz A, Kaczmarek U: Analiza porównawcza ultrastrukturalnego obrazu i składu chemicznego wczesnej zmiany próchnicowej i zdrowych tkanek twardych zębów. Dent Med Probl 2005, 42, 443–448.
  13. Torres CRG, Borges AB, Torres LMS, Gomes IS, Oliveira RS: Effect of caries infiltration technique and fluoride therapy on the colour masking of white spot lesions. J Dent 2011, 39, 202–207.
  14. Skucha-Nowak M, Tanasiewicz M, Gibas M, Twardawa H: Analysis of the composition of preparations used as a barrier to protect tissues of the patient against the influence of the environment in the oral cavity. Pol J Environ Stud 2013, 22, 53–57.
  15. Skucha-Nowak M, Tanasiewicz M, Gibas M, Twardawa H: Barriers securing the patient’s own tissues against of the oral cavity environment than can be used in low invasive dentistry. Pol J Environ Stud 2012, 21, 25–29.
  16. Buskes JAKM, Christofferson J, Arends J: Lesion formation and lesion remineralization in enamel under constant composition conditions. A new technique with application. Caries Res 1985, 19, 490–496.
  17. Loba-Jakubowska E: Bisfosfoniany – nadzieja na skuteczne leczenie osteoporozy w wieku rozwojowym. Przegl Pediatr 2003, 33, 261–264.
  18. Chmielewska E: Nowe bisfosfoniany jako potencjalne leki przeciw osteoporozie. Praca doktorska. Politechnika Wrocławska 2010.
  19. Chu JS: The role of fluoride in mineral dissolution and remineralization of dental enamel. Praca doktorska. The University of Utah 1989.
  20. Fan Y: Effects of different finishing procedures and materials on surface roughness of infiltrated subsurface bovine enamel lesions. Praca doktorska, Universitätsmedizin, Berlin 2010.
  21. Buzalaf MA, Hannas AR, Magalhăes AC, Rios D, Honório HM, Delbem AC: pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. J Appl Oral Sci 2010, 18, 316–334.
  22. Meyer-Lueckel H, Paris S: Improved Resin Infiltration of Natural Caries Lesions. J Dent Res 2008, 87, 1112–1116.
  23. Meyer-Lueckel H, Paris S, Kielbassa AM: Surface Layer Erosion of Natural Caries Lesions with Phosphoric and Hydrochloric Acid Gels in Preparation for Resin Infiltration. Caries Res 2007, 41, 223–230.
  24. Meyer-Lueckel H, Paris S: Progression of Artificial Enamel Caries Lesions after Infiltration with Experimental Light Curing Resins. Caries Res 2008, 42, 117–124.
  25. Yang F, Mueller J, Kielbassa A: Surface substance loss of subsurface bovine enemel lesions after different steps of the resinous infiltration technique: 3D topography analysis. Odontology 2012, 100, 172–180.
  26. Mueller J, Yang F, Neumann K, Kielbassa A: Surface tridimensional topography analysis of materials and finishing procedures after resinous infiltration of subsurface bovine enamel lesions. Quintessence Int 2011, 42, 135–147.
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