Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 3, May-June, p. 437–440

doi: 10.17219/acem/34474

Publication type: original article

Language: English

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Typeability of DNA in Touch Traces Deposited on Paper and Optical Data Discs

Ireneusz Sołtyszewski1,A,D,E, Michał Szeremeta2,B,C,D,E, Małgorzata Skawrońska2,B,C, Anna Niemcunowicz-Janica2,E,F, Witold Pepiński2,A,D,F

1 Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury in Olsztyn, Poland

2 Department of Forensic Medicine, Medical University of Bialystok, Poland

Abstract

Background. Nucleated epithelial cells that are transferred by casual touching and handling of objects are the primary source of biological evidence that is found in high-volume crimes. Cellular material associated with touch traces usually contains low levels of DNA template making it challenging to acquire an informative profile.
Objectives. The main purpose of this study was to examine the efficacy of DNA typing in fingerprints deposited on optical data discs and the office paper.
Material and Methods. Latent fingerprints were made by 60 subjects of both sexes (30 males and 30 females). A highly effective DNA extraction method with QIAamp DNA Mini Kit (Qiagen) and an increased sensitivity PCR by AmpFlSTR® NGM™ Amplification Kit (Applied Biosystems) carried out at standard 30 cycles and at increased 34 cycles were used.
Results. The mean value of total DNA recovery was 0.4 ng from CDs/DVDs and 0.3 ng from the office paper. Amplification of Low Template DNA (LT-DNA) resulted in improved analytical success by increasing the number of PCR cycles from standard 30 to 34. On the other hand, the increased PCR cycles resulted in allele drop-ins showing additional peaks, the majority of which were outside the stutter positions.
Conclusion. Rigorous procedures and interpretation guidelines are required during LT-DNA for producing reliable and reproducible DNA profiles for forensic purposes.

Key words

forensic medicine, touch traces, genotyping techniques.

References (23)

  1. Ludwig A, Fraser J: Effective use of forensic science in volume crime investigations: identifying recurring themes in the literature. Sci Justice 2014, 54, 81–88.
  2. van Oorschot RAH, Jones MK: DNA fingerprints from fingerprints. Nature 1997, 387, 767.
  3. Roman JK, Reid S, Reid J, Chalfin A, Adams W, Knight C: The DNA Field Experiment: Cost-Effectiveness Analysis of the Use of DNA in the Investigation of High-Volume Crimes. NIJ Final Report No. 222318, 2008.
  4. Daly DJ, Murphy C, McDermott SD: The transfer of touch DNA from hands to glass, fabric and wood. Forensic Sci Int Genet 2012, 6, 41–46.
  5. Findlay I, Frazier R, Taylor A, Urquhart A: Single cell DNA fingerprinting for forensic applications. Nature 1997, 389, 555–556.
  6. Kloosterman AD, Kersbergen P: Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci. J Soc Biol 2003, 197, 351–359.
  7. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J: An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci Int 2000, 112, 17–40.
  8. Prinz M, Schiffner L, Sebestyen JA, Bajda E, Tamariz J, Shaler RC, Baum H, Caragine T: Maximization of STR DNA typing success for touched objects. Int Congress Ser 2006, 1288, 651–653.
  9. Smith PJ, Ballantyne J: Simplified low-copy-number DNA analysis by post-PCR purification. J Forensic Sci 2007, 52, 820–829.
  10. Caddy B, Taylor G, Linacre A: A review of the science of low template DNA analysis. 2008. At http://bioforensics. com/articles/Caddy_Report.pdf.
  11. Alonso A, Martin P, Albarrán C, Garcia P, Fernandez de Simon L, Jesús Iturralde M, Fernández-Rodriguez A, Atienza I, Capilla J, García-Hirschfeld J, Martinez P, Vallejo G, García O, García E, Real P, Alvarez D, León A, Sancho M: Challenges of DNA profiling in mass disaster investigations. Croatian Medical Journal 2005, 46, 540–548.
  12. Butler JM, Shen Y, McCord BR: The development of reduced size STR amplicons as tool for analysis of degraded DNA. J Forensic Sci 2003, 48, 1054–1064.
  13. Wiegand P, Kleiber M: Less is more – length reduction of STR amplicons using redesigned primers. Int J Leg Med 2001, 114, 285–287.
  14. Coble MD, Butler JM: Characterization of new miniSTR loci to aid analysis of degraded DNA. J Forensic Sci 2005, 50, 43–53.
  15. Budowle B, Eisenberg AJ, van Daal A: Validity of low copy number typing and applications to forensic science. Croat Med J 2009, 50, 207–221.
  16. Gill P, Brown RM, Fairley M, Lee L, Smyth M, Simpson N, Irwin B, Dunlop J, Greenhalgh M, Way K, Westacott EJ, Ferguson SJ, Ford LV, Clayton T, Guiness J: National recommendations of the Technical UK DNA working group on mixture interpretation for the NDNAD and for court going purposes. Forensic Sci Int Genet 2008, 2, 76–82.
  17. Gill P: Application of low copy number DNA profiling. Croat Med J 2001, 42, 229–232.
  18. Buckleton J: Validation issues around DNA typing of low level DNA. Forensic Sci Int Genet 2009, 3, 255–260.
  19. Caragine T, Mikulasovich R, Tamariz J, Bajda E, Sebestyen J, Baum H, Prinz M: Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler. Croat Med J 2009, 50, 250–267.
  20. Gill P: Role of Short Tandem Repeat DNA in forensic casework in the UK – past, present and future perspectives. Biotechniques 2002, 32, 366–385.
  21. Griesdale KS, van Daal A: Comparison of STR profiling from low template DNA extracts with and without the consensus profiling metod. Invest Genet 2012, 3:14, doi: 10.1186/2041-2223-3-14.
  22. Lowe A, Murray C, Whitaker J, Tully G, Gill P: The propensity of individuals to deposit DNA secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci Int 2002, 129, 25–34.
  23. Allen RW, Pogemiller J, Joslin J, Gulick M, Pritchard J: Identification through typing of DNA recovered from touch transfer evidence: parameters affecting yield of recovered human DNA. J Forensic Int 2008, 58, 33–41.