Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 2, March-April, p. 251–256

doi: 10.17219/acem/40454

Publication type: original article

Language: English

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Gene Polymorphisms of Tumor Necrosis Factor Alpha and Antioxidant Enzymes in Bronchial Asthma

Milena Despotovic1,A,C,D, Tatjana J. Stoimenov1,A,F, Ivana Stankovic2,B,E, Dusica Pavlovic1,F, Dusan Sokolovic1,E, Tatjana Cvetkovic1,E, Gordana Kocic1,E, Jelena Basic1,C, Andrej Veljkovic1,B, Branka Djordjevic1,B

1 Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia

2 Clinic for Pulmonary Diseases and Tuberculosis, Nis Clinical Center, University of Nis, Knez Selo, Serbia

Abstract

Background. Bronchial asthma is an inflammatory disease resulting from a combination of genetic and environmental factors. Single nucleotide polymorphisms in the regulatory regions of cytokine and antioxidant enzyme genes may affect cytokine production and enzyme activity, and thus play a contributory role in asthma pathogenesis.
Objectives. The aim of this study was to examine the association of manganese superoxide dismutase (MnSOD) Ala16Val, catalase (CAT) A-21T and tumor necrosis factor alpha (TNF-α) G-308A polymorphisms with bronchial asthma.
Material and Methods. A total of 79 patients with asthma and 95 healthy controls were screened for MnSOD Ala16Val, CAT A-21T and TNF-α G-308A polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Results. The results obtained showed significantly higher prevalence of the MnSOD ValVal genotype (χ2 = 14.463, df = 2, p = 0.001) and MnSOD 16Val allele (χ2 = 12.862, p = 0.026, OR = 0.451, 95% CI = 0.291–0.699) in patients with asthma compared to controls. The genotype and allele frequencies distribution of CAT A-21T and TNF-α G-308A gene polymorphisms did not show differences between patients and controls.
Conclusion. Our results show an association of MnSOD Ala16Val genetic polymorphism with asthma in a Serbian population and suggest a protective role of the MnSOD 16Ala allele.

Key words

bronchial asthma, gene polymorphism, tumor necrosis factor alpha, catalase, manganese superoxide dismutase.

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