Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1 (5-Year IF – 2.0)
Journal Citation Indicator (JCI) (2023) – 0.4
Scopus CiteScore – 3.7 (CiteScore Tracker 3.8)
Index Copernicus  – 171.00; MNiSW – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 1, January-February, p. 17–24

Publication type: original article

Language: English

In Vitro Effects of Candida albicans and Aspergillus fumigatus on Dendritic Cells and the Role of Beta Glucan in this Effect

Isil Fidan1,A,C,D,F, Ayse Kalkanci1,B,C,E, Emine Yesilyurt1,B,C, Berna Erdal1,B,C

1 Gazi University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey

Abstract

Background. Dendritic cells (DCs) are able to initiate and regulate the immune response to fungal infections. β-glucan stimulates the immune system, modulating cellular and humoral immunity. It has a beneficial effect in fighting fungal infections.
Objectives. We investigated the in vitro effect of C.albicans and A.fumigatus infection on human DCs. The cytokine levels were determined by ELISA.
Material and Methods. Human PBMCs isolation was performed by Ficoll-hypaque density gradient centrifugation method. DCs maturation was analysed by using flow cytometry. The cytokine levels were determined by ELISA.
Results. DCs stimulated by C. albicans and A. fumigatus induced DC maturation by increasing CD80 and CD86 co-stimulatory molecules. DCs stimulated by fungi produced IL-8 and IL-12p70. Whereas IL-10 production from the stimulated DCs did not differ from uninfected DCs. Also, the addition of β-glucan to the DCs stimulated by fungi promoted the activation and maturation of DCs.
Conclusion. Our results suggest that DCs are capable of initiating an innate and adaptive immune response against fungal infections. In addition, β-glucan can be used as a novel stimulator to DC-based vaccination against fungal infections.

Key words

dendritic cells, fungal infection, β-glucan.

References (19)

  1. Faivre V, Lukaszewicz, Alves A, Charron D, Payen D, Haziot A: Accelerated in vitro differentiation of blood monocytes into dendritic cells in human sepsis. Clin Exp Immunol 2007, 147, 426–439.
  2. Sallusto F, Lanzavecchia A: Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/Macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor α. J Exp Med 1994, 179, 1109–1118.
  3. Gafa V, Remoli MR, Giacomini E, Gagliardi MC, Lande R, Severa M, Grillot R, Coccia EM: In vitro infection of human dendritic cells by Aspergillus fumigatus conidia triggers the secretion of chemokines for neutrophil and Th1 lymphocyte recruitment. Microb Infect 2007, 9, 971–980.
  4. Sundquist M, Rydström A, Wick MJ: Immunity to salmonella from a dendritic point of view. Cell Microbiol 2004, 6, 1–11.
  5. Flohe SB, Agrawal H, Schmitz D, Gertz M, Flohe S, Schade FU: Dendritic cells during polymicrobial sepsis rapidly mature but fail to initiate a protective Th1-type immune response. J Leuko Biol 2006, 79, 473–481.
  6. Perruccio K, Boza S, Montagnoli C, Bellocchio S, Aversa F, Martelli M, Bistoni F, Velardi A, Romani L: Prospects for dendritic cell vaccination against fungal infections in hematopoetic transplantation. Blood Cells, Mol Dis 2004, 33, 248–255.
  7. Brakhage AA, Bruns S, Thywissen A, Zipfel PF, Behnse J: Interaction of phagocytes with filamentous fungi. Curr Opin Microbiol 2010, 13, 409–415.
  8. Lull C, Wichers HJ, Savelkoul FJ: Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflamm 2005, 2, 63–80.
  9. Aiba S, Tagami H: Dendritic cell activation induced by various stimuli, e.g. exposure to microorganisms, their products, cytokines, and simple chemicals as well as adhesion to extracellular matrix. J Dermatol Sci 1999, 20, 1–13.
  10. Granucci F, Andrews DM, Degli-Esposti MA: Ricciardi-Castagnoli P. IL-2 mediates adjuvant effect of dendritic cells. Trends Immunol 2002, 23, 169–171.
  11. Netea MG, Gijzen K, Coolen N, Verschueren I, Figdor C, Van der Meer JWM, Torensma R, Kullberg BJ: Human dendritic cells are less potent at killing Candida albicans than both monocytes and macrophages. Microb Infect 2004, 6, 985–989.
  12. Buentke E, Scheynius A: Dendritic cells and fungi. APMIS 2003, 111, 789–796.
  13. Lin YL, Lee SS, Hou SM, Chiang BL: Polysaccharide purified from Ganoderna lucidum induces gene expression changes in human dendritic cells and promotes T Helper 1 immune response in BALB/c mice. Mol Pharmacol 2006, 70, 637–644.
  14. Romagnoll G, Nisini R, Chlani P, Marlotti S, Teloni R, Cassone A, Torosantucci A: The interaction of human dendritic cells with yeast and germ-tube forms of Candida albicans leads to efficient fungal processing, dendritic cell maturation, and acquisition of a Th1 response-promoting function. J Leukol Biol 2004, 75, 117–126.
  15. Kikuchi T, Ohno N, Ohno T: Maturation of dendritic cells induced by Candida β-D-glucan. Int Immunopharma 2002, 2, 1503–1508.
  16. Gafa V, Lande R, Gagliardi MC, Severa M, Giacomii E, Remoli ME: Human dendritic cells following Aspergillus fumigatus infection Express the CCR7 receptor and a differential pattern of interleukin-12 (IL-12), Il-23, and IL-27 cytokines, which lead to a Th1 response. Infect Immun 2006, 74, 148–1489.
  17. Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of β-glucans on the immune system. Medicina 2007, 43, 597–606.
  18. Awasthi S, Magee DM: Differences in expression of cell surface co-stimulatory molecules, Toll-like receptor genes and secretion of IL-12 by bone marrow-derived dendritic cells from susceptible and resistant Mouse strains in response to Coccidioides posadasii. Cell Immunol 2004, 231, 49–55.
  19. Carmona E, Vassallo R, Vuk-Pavlovic Z, Standing JE, Theodore TJ, Limper AH: Pnuemocystis cell wall β-Glucans induce dendtitic cell co-stimulatory molecule expression and inflammatoryactivation through a FasFas ligand mechanism. J Immunol 2007, 177, 459–467.
© Wroclaw Medical University