Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1 (5-Year IF – 2.0)
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Index Copernicus  – 171.00; MNiSW – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2013, vol. 22, nr 6, November-December, p. 785–794

Publication type: original article

Language: English

Proteomic Analysis of the Immunosuppressive Effects of Mesenchymal Stem Cells in a Rat Heart Transplantation Model

Analiza proteomiczna działania immunosupresyjnego mezenchymalnych komórek macierzystych na modelu zwierzęcym z wykorzystaniem serca szczurów

Shuo-Ming Wu1,B,C,D, Wen-Xiong Zhang1,B,C,D, Ming-Hui Wang1,B,C, Hui-Zhong Zhang1,A,E,F, Duo-Guang Wu1,C, Zhi-Juan Zhou2,C, Li-Hua Xiong1,B,C

1 Department of Heart Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China

2 Department of Heart Surgery, Fifth Affiliated Hospital of Sun Yat-sen University, China

Abstract

Background. Some reports suggest mesenchymal stem cells (MSCs) have immunosuppressive properties. However, conflicting evidence regarding the role of MSCs has emerged.
Objectives. To gain a better understanding of the immunosuppressive properties of mesenchymal stem cells (MSCs) in a rat heart transplantation model.
Material and Methods. MSCs were obtained from the femoral and tibial bone marrow of Sprague-Dawley rats and cultured. Heart-transplanted rats were allocated into a MSC-treated group and 2 control groups. On postoperative day 7, 1 rat was sacrificed and the pathological changes of heart tissues were assessed. Serum proteomic spectra were generated by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS).
Results. Rat MSCs displayed the typical spindle-shaped morphology in culture and significantly prolonged the graft survival up to 33.25 ± 2.54 days compared with controls (19.75 ± 1.56 and 11.16 ± 1.34 days, respectively). Pathological analysis showed the inflammatory cell infiltration in the MSC-treated group was significantly reduced. SELDI analysis showed that 5 protein/peptide peaks with M/Z 1272.33, 1986.65, 2323.42, 5375.59 and 12968.11 were up-regulated in the MSC-treated group (P < 0.001).
Conclusion. Donor-derived MSCs clearly alleviate acute rejection following heart transplantation in rats and significantly prolong the isograft survival time

Key words

rat heart transplantation, immunosuppression, mesenchymal stem cells, SELDI ProteinChip, decision tree.

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