Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2013, vol. 22, nr 3, May-June, p. 403–409

Publication type: original article

Language: English

An Evaluation of Nitric Oxide, Folate, Homocysteine Levels and Lipid Peroxidation in Postmenopausal Osteoporosis

Ocena stężenia tlenku azotu, kwasu foliowego, homocysteiny i peroksydacji lipidów w osteoporozie pomenopauzalnej

Veysi Akpolat1,F, H. Murat Bilgin2,A,B,D,E, M. Yusuf Celik3,C, Mahmut Erdemoglu4,B, Birgul Isik5,A,B

1 Department of Biophysics, Bone Mineral Densitometry Unit, Faculty of Medicine, Dicle University, Turkey

2 Department of Physiology, Faculty of Medicine, Dicle University, Turkey

3 Department of Biostatistics, Faculty of Medicine, Dicle University, Turkey

4 Department of Obstetrics and Gynecology, Faculty of Medicine, Dicle University, Turkey

5 Department of Biochemistry, Faculty of Medicine, Dicle University, Turkey


Objectives. In the current study, the risk coefficients of nitric oxide (NO), folate, homocysteine levels and lipid peroxidation in postmenopausal osteoporotic women were determined.
Material and Methods. Bone mineral density was determined by dual-energy X-ray absorptiometry. The levels of serum lipid peroxidation products such as the thiobarbituric acid adduct of malondialdehyde (MDA) were measured spectrophotometrically. Plasma folate and homocysteine (Hcy) levels were measured by enzyme chemi- luminescence immunoassay. Plasma nitrite levels were measured with the Griess reaction
Results. The odds ratios and 95% Confidence Intervals (CI) of the variables MDA, folate, NO, body-mass index (BMI), menopause age and age were found significant. MDA, NO and folate variables were found statistically sig- nificant in the analysis of receiver operating charecteristic (ROCs). The areas under the curve (AUCs, 95% CI) of MDA, NO and folate were found to be statistically significant.
Conclusions. The current study indicates that NO, MDA and folate are risk variables for postmenopausal osteo- porosis (Adv Clin Exp Med 2013, 22, 3, 403–409).


Cel pracy. W pracy oceniono czynniki ryzyka, takie jak stężenie tlenku azotu (NO), kwasu foliowego, homocyste- iny i peroksydację lipidów, w okresie pomenopauzalnym u kobiet chorych na osteoporozę.
Materiał i metody. Gęstość mineralną kości zmierzono za pomocą dwuwiązkowej absorpcjometrii rentgenowskiej. Stężenia produktów peroksydacji lipidów, takich jak addukt aldehydu malonowego (MDA) i kwasu tiobarbituro- wego, zmierzono spektrofotometrycznie. Stężenie kwasu foliowego i homocysteiny (Hcy) zmierzono za pomocą testu immunoenzymatycznego chemiluminescencji. Stężenie azotynów w osoczu zmierzono za pomocą reakcji Griessa.
Wyniki. Ilorazy szans i 95% przedziały ufności (CI) zmiennych MDA, kwas foliowy, NO, wskaźnik masy ciała (BMI), wiek menopauzy oraz wiek były znaczące. Stwierdzono, że zmienne MDA, NO i kwas foliowy są istotne statystycznie w analizie krzywej ROC. Obszary pod krzywą (AUC, 95% CI) MDA, NO i kwasu foliowego okazały się istotne statystycznie.Wnioski. Wyniki badania wskazują, że NO, MDA i kwas foliowy są czynnikami ryzyka osteoporozy pomenopau- zalnej (Adv Clin Exp Med 2013, 22, 3, 403–409).


Key words

Key words: nitric oxide, folate, malondialdehyde, homocysteine, osteoporosis.

Słowa kluczowe

Słowa kluczowe: tlenek azotu, kwas foliowy, aldehyd malonowy, homocysteina, osteoporoza.

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