Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2012, vol. 21, nr 5, September-October, p. 591–599

Publication type: original article

Language: English

Invasive Properties, Adhesion Patterns and Phylogroup Profiles Among Escherichia Coli Strains Isolated from Children with Inflammatory Bowel Disease

Zdolności adhezyjne i inwazyjne oraz przynależność do grup filogenetycznych szczepów E. coli izolowanych od dzieci z nieswoistymi zapaleniami jelit

Beata Sobieszczańska1,A,C,D,F, Anna Duda-Madej1,B,C, Michał Turniak1,B,C, Roman Franiczek1,B,C, Urszula Kasprzykowska1,B,C, Anna K. Duda1,B,C, Marta Rzeszutko2,B,C, Barbara Iwańczak3,E

1 Department of Microbiology, Wroclaw Medical University, Wrocław, Poland

2 Department of Patomorphology, Wroclaw Medical University, Wrocław, Poland

3 2nd Department and Clinic of Pediatrics and Gastroenterology, Wroclaw Medical University, Wrocław, Poland

Abstract

Background. A great deal of evidence indicates a link between Escherichia coli (E. coli) and Crohn’s disease in adult patients, but there is lack of information on the association of these bacilli with inflammatory bowel disease (IBD) among children.
Objectives. The study was carried out to determine the distribution of phylogenetic group, the adherence patterns and invasive properties of E. coli isolated from children with IBD and non-IBD chronic bowel diseases.
Material and Methods. A total of 22 E. coli isolated from biopsy specimens from children with IBD and 21 E. coli strains obtained from children with indeterminate colitis and intestinal polyps were examined for adherence and internalization to the Int407 cell line. Genes involved in epithelial cell invasion and genes specific to E. coli phylogroups were determined by polymerase chain reaction (PCR).
Results. The undefined adherence pattern predominated among the isolated E. coli, although most of them demonstrated the afaD and aggB genes encoding invasions of diffusely adhering and enteroaggregative E. coli. Regardless of the clinical entity, most E. coli were internalized by Int407 epithelial cells and belonged to the B2 and D phylogroups.
Conclusion. The wide distribution of adhesive E. coli capable of entering Int407 cells but also having genes encoding adhesins and invasins characteristic to pathogenic E. coli strains seems to indicate that these E. coli may represent a large group of pathogenic E. coli strains contributing to chronic intestinal disorders.

Streszczenie

Wprowadzenie. Wiele wyników badań wskazuje na związek Escherichia coli z chorobą Leśniowskiego-Crohna u osób dorosłych, jednak nadal brakuje danych na temat roli tych pałeczek w nieswoistych zapaleniach jelit (IBD) u dzieci.
Cel pracy. Charakterystyka zdolności adhezyjnych i inwazyjnych oraz przynależność do grup filogenetycznych szczepów E. coli izolowanych od dzieci z nieswoistymi zapaleniami jelit oraz innymi przewlekłymi chorobami przewodu pokarmowego.
Materiał i metody. Test adhezji i inwazji in vitro do komórek nabłonka jelita linii Int407 wykonano dla 43 szczepów E. coli izolowanych z bioptatów błony śluzowej jelita 22 dzieci z rozpoznaną chorobą Leśniowskiego-Crohna i wrzodziejącym zapaleniem jelita grubego oraz 21 dzieci z nieokreślonym zapaleniem jelita i polipami jelit. Geny kodujące inwazyny oraz geny klasyfikujące badane szczepy do konkretnej grupy filogenetycznej wykrywano testem PCR.
Wyniki. Wśród badanych izolatów E. coli przeważał nieokreślony typ adhezji, chociaż większość szczepów posiadała geny kodujące inwazyny, tj. afaD i aggB typowe dla szczepów o rozsianym i agregacyjnym typie adhezji. Niezależnie od jednostki chorobowej, z której izolowano badane szczepy, większość z nich była internalizowana przez komórki nabłonka jelita i należała do grup filogenetycznych B2 i D.
Wnioski. Z uwagi na to, że większość badanych szczepów E. coli wykazała zdolność adhezji i inwazji do komórek nabłonka jelita oraz prezentowała geny kodujące inwazyny typowe dla patogennych szczepów E. coli, prawdopodobnie badane izolaty mogą reprezentować większą grupę szczepów związanych z przewlekłymi chorobami przewodu pokarmowego.

Key words

inflammatory bowel disease, invasion, E. coli, adherence pattern, invasion genes, phylogroups

Słowa kluczowe

nieswoiste zapalenie jelit, inwazja, E. coli, typ adhezji, geny inwazji, filogrupy

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