Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
Scopus CiteScore – 3.4 (CiteScore Tracker 3.4)
Index Copernicus  – 161.11; MEiN – 140 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2012, vol. 21, nr 4, July-August, p. 441–446

Publication type: original article

Language: English

Effects of Maternal Smoking on Fetal Organs

Wpływ palenia tytoniu przez matkę na narządy wewnętrzne płodu

Abdulbaki Karaoglu1,, Turan Tunc2,, Gokhan Aydemir3,, Onder Ongoru4,, Secil Aydinoz3,, Bulent Uysal5,, Galip Erdem1,, Ahmet Guven6,, Emin Oztas7,, S. Umit Sarici2,

1 Gulhane Military Medical Faculty, Department of Pediatrics, Ankara, Turkey

2 Gulhane Military Medical Faculty, Department of Pediatrics, Division of Neonatology, Ankara, Turkey

3 GATA Haydarpasa Teaching Hospital Department of Pediatrics, Istanbul, Turkey

4 Gulhane Military Medical Faculty, Department of Pathology, Ankara, Turkey

5 Gulhane Military Medical Faculty, Department of Physiology, Ankara, Turkey

6 Gulhane Military Medical Faculty, Department of Pediatric Surgery, Ankara, Turkey

7 Gulhane Military Medical Faculty, Department of Medical Histology and Embrology, Ankara, Turkey

Abstract

Background. Unfavorable effects of in-utero smoke exposure have been shown in several studies.
Objectives. In this experimental study, the authors aimed at showing detrimental effects of cigarette smoke on fetal tissues by assessing apoptosis that is detected by performing TUNEL staining.
Material and Methods. Designed groups were smoke exposed rats before and during pregnancy and control groups. Rat offsprings were sacrificed when they were 12 days old.
Results. Lung, kidney, adrenal and gonad tissues were harvested for histopathologic analysis and assessed by TUNEL (Terminal dUTP Nick End Labeling) staining.
Conclusion. Smoke exposure caused increased apoptotic activity in lung parenchyma of study groups.

Streszczenie

Wprowadzenie. Niekorzystne skutki wewnątrzmacicznej ekspozycji płodu na dym papierosowy wykazano w wielu badaniach.
Cel pracy. W badaniu doświadczalnym autorzy mieli na celu pokazanie negatywnego wpływu dymu papierosowego na tkanki płodu przez ocenę apoptozy wykrywanej za pomocą barwienia metodą TUNEL.
Materiał i metody. Do badania włączono szczury narażone na dym tytoniowy przed i w czasie ciąży oraz grupę kontrolną. Potomstwo szczurów uśmiercono, gdy miało 12 dni.
Wyniki. Pobrano tkanki płuc, nerek, nadnerczy i gonad do analizy histopatologicznej i oceniono za pomocą barwienia metodą TUNEL (Terminal dUTP Nick End Labeling).
Wnioski. Narażenie na dym papierosowy spowodowało zwiększoną aktywność apoptozy w miąższu płuc w badanych grupach.

Key words

cigarette smoking, lung, apoptosis

Słowa kluczowe

palenie tytoniu, płuca, apoptoza

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