Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2011, vol. 20, nr 6, November-December, p. 721–727

Publication type: original article

Language: English

Pulsing Electromagnetic Field and Death of Proliferating Peripheral Blood Mononuclear Cells from Patients with Acute Myelogenic Leukemia

Pulsacyjne pole elektromagnetyczne a śmierć komórkowa proliferujących leukocytów jednojądrzastych pochodzących od pacjentów z ostrą białaczką mieloidalną

Jolanta Kaszuba-Zwoińska1,, Edyta Zdziłowska2,, Paulina Chorobik3,, Zofia Słodowska-Hajduk1,, Kajetan Juszczak1,, Wiesław Zaraska4,, Piotr J. Thor1,

1 Department of Pathophysiology, Jagiellonian University – Medical College, Cracow, Poland

2 Clinic of Hematology, Jagiellonian University – Medical College, Cracow, Poland

3 Department of Immunology, Jagiellonian University – Medical College, Cracow, Poland

4 Institute of Electron Technology, Cracow, Poland

Abstract

Background. A pulsing electromagnetic field (PEMF) influenced the viability of proliferating in vitro peripheral blood mononuclear cells isolated from Crohn’s disease patients by induction of cell death but did not cause any vital changes in the cells from healthy donors. Experiments with lymphoid cell line U937 have shown a protective effect of PEMF on puromycin-treated cells.
Objectives. The current study aimed to investigate the influence of PEMF on native proliferating leukocytes originating from newly-diagnosed acute myelogenous leukemia (AML) patients.
Material and Methods. The effects of exposure to PEMF were studied in peripheral blood mononuclear cells from 8 patients with AML. Peripheral blood mononuclear cells (PBMCs) were stimulated with three doses of PEMF for 3h each with 24h intervals. After the last stimulation, the cells were double stained with Annexin V and 7-aminoactinomycin D (7-AAD) dye to estimate viability by flow cytometry analysis.
Results. Results indicated an increase of Annexin V-positive as well as double stainedand 7-AAD-positive cells after exposure to threefold PEMF stimulation.
Conclusion. A low-frequency pulsing electromagnetic field induces cell death in native proliferating cells isolated from AML patients. The increased vulnerability of proliferating PBMCs may be potentially applied in the therapy of AML.

Streszczenie

Wprowadzenie. Pulsacyjne pole elektromagnetyczne (PEMF) wpływa na żywotność proliferujących in vitro jednojądrzastych leukocytów krwi obwodowej (PBMCs) izolowanych od pacjentów z chorobą Crohna przez wywołanie śmierci komórkowej, a nie powoduje zmian w żywotności komórek od zdrowych dawców. Eksperymenty prowadzone na linii komórkowej U937 wykazały ochronny wpływ PEMF na komórki poddane działaniu puromycyny.
Cel pracy. Bieżące badania mają na celu przetestowanie wpływu PEMF na natywnie proliferujące leukocyty pochodzące od pacjentów z AML.
Materiał i metody. Działanie wywierane przez PEMF było badane na jednojądrzastych leukocytach krwi obwodowej uzyskanej od 8 nowo zdiagnozowanych pacjentów. PBMCs były poddawane 3-krotnie po 3 godz. jednorazowo działaniu PEMF w odstępach 24 godz. Po ostatniej stymulacji wybarwiono komórki aneksyną V i 7-amino-aktynomycyną D do analizy żywotności metodą cytometrii przepływowej.
Wyniki. Uzyskano wzrost liczby komórek pozytywnie barwiących się aneksyną V, 7-amino-aktynomycną D oraz obydwoma markerami po 3-krotnej ekspozycji PEMF.
Wnioski. Pulsacyjne pole elekromagnetyczne wywołuje śmierć komórkową w natywnie proliferujących komórkach pochodzących od pacjentów z AML. Zwiększona podatność proliferujących leukocytów jednojądrzastych na działanie PEMF potencjalnie może być wykorzystana do leczenia AML.

Key words

pulsing electromagnetic field, acute myelogenous leukemia, apoptosis, necrosis

Słowa kluczowe

pulsacyjne pole elektromagnetyczne, ostra białaczka mieloidalna, apoptoza, martwica

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