Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2011, vol. 20, nr 6, November-December, p. 677–682

Publication type: original article

Language: English

The Level of Calcium and Magnesium in Blood of Rats Receiving Various Doses of Silicon

Stężenie wapnia i magnezu we krwi szczurów otrzymujących różne dawki krzemu

Anna Boguszewska-Czubara1,, Anna Hordyjewska1,, Kazimierz Pasternak1,, Małgorzata Kiełczykowska1,, Jadwiga Jaworska-Adamu2,, Radosław Szalak2,

1 Department of Medical Chemistry, Medical University of Lublin, Poland

2 Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Poland

Abstract

Background. Silicon, the third most abundant trace element of the human body, is listed as an essential one. It is especially associated with connective tissues as it has been found to take part in bone development, collagen formation and mineralization of bone matrix. Silicon is also implicated in mammalian hormonal control and in protecting against heart disease in humans.
Objectives. The influence of different doses of orally-administered silicon on calcium and magnesium concentrations in the blood of experimental animals was evaluated.
Material and Methods. The experiment was carried out on male Wistar rats. A control group was given distilled water to drink. The rats in group 0 were given a solution of sodium hydroxide (0.001 mol/L), whereas animals in groups 1, 2 and 3 received solutions of orthosilicic acid of three different concentrations (0.05%, 0.5% and 1%) as the only drinking fluids. Blood was collected after 4 and then 8 weeks of the experiment. Determination of calcium and magnesium concentrations in the blood was performed by the ICP-AES method.
Results. Silicon administration caused an increase in blood calcium concentration after 4 as well as after 8 weeks of the experiment. Four-week-long silicon intoxication caused a decrease in blood magnesium concentrations, whereas an increase in blood magnesium level in groups 0, 1 and 3 and a decrease in group 2 after 8 weeks of the experiment were noted.
Conclusion. Silicon was found to significantly influence metabolism of calcium and magnesium. Its interaction with calcium during the process of bones mineralization suggests that Si supplementation may be helpful in preventing osteoporosis in postmenopausal women whose calcium intake is insufficient. Homeostasis in mineral metabolism and balance between elements are very important matters, therefore silicon metabolism and its interactions with other elements and nutrients should be further investigated.

Streszczenie

Wprowadzenie. Krzem jest niezbędny do prawidłowego funkcjonowania organizmów żywych. Jest pierwiastkiem występującym w śladowych ilościach i zajmuje wśród nich trzecie miejsce co do rozpowszechnienia. Szczególnie istotną rolę odgrywa w funkcjonowaniu tkanek łącznych – rozwoju kości i tworzeniu kolagenu. Badania wykazują, że krzem odgrywa również pewną funkcję w prawidłowej gospodarce hormonalnej organizmu oraz może działać ochronnie w przypadkach chorób kardiologicznych u ludzi.
Cel pracy. Badanie wpływu doustnego podawania różnych dawek krzemu na stężenia wapnia i magnezu we krwi szczurów.
Materiał i metody. Badanie przeprowadzono na szczurach samcach rasy Wistar. Grupa kontrolna otrzymywała do picia wodę redestylowaną. Zwierzętom z grupy 0 podawano wodny roztwór wodorotlenku sodu (0,001 mol/l), a szczurom z grup 1, 2 i 3 roztwory kwasu ortokrzemowego o różnych stężeniach (0,05%, 0,5% i 1%) jako jedyne płyny do picia. Krew do badań pobierano po 4 i 8 tygodniach trwania doświadczenia. W pobranych próbkach krwi oznaczano stężenia wapnia i magnezu metodą ICP-AES.
Wyniki. Podawanie krzemu spowodowało zwiększenie stężenia wapnia we krwi zarówno po 4, jak i 8 tygodniach trwania eksperymentu. Stężenie magnezu zmniejszyło się po 4-tygodniowej intoksykacji, a po 8 tygodniach nastąpił wzrost stężenia tego pierwiastka we krwi zwierząt grup 0, 1 i 3 oraz spadek w grupie 2.
Wnioski. Badania wykazały, że krzem znacząco wpływa na metabolizm wapnia i magnezu. Interakcje między wapniem i krzemem, zachodzące w procesie mineralizacji kości, sugerują, że suplementacja krzemu może zapobiegać osteoporozie u kobiet po menopauzie, u których pobranie wapnia nie jest dostateczne. Zachowanie homeostazy metabolizmu biopierwiastków i równowaga między nimi jest niezwykle istotną kwestią, dlatego metabolizm krzemu i jego interakcje z innymi pierwiastkami i składnikami pożywienia powinny być przedmiotem dalszych badań.

Key words

calcium, magnesium, silicon, rats, chronic toxicity

Słowa kluczowe

wapń, magnez, krzem, szczury, toksyczność przewlekła

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