Advances in Clinical and Experimental Medicine
2009, vol. 18, nr 4, July-August, p. 401–405
Publication type: original article
Language: English
Triclosan−Coated Sutures in Oral Surgery
Nici chirurgiczne pokrywane triklosanem w chirurgii jamy ustnej
1 National Medicines Institute, Warsaw, Poland
2 Biomedical Engineering Laboratory, Warsaw University of Technology, Warsaw, Poland
3
Abstract
Background. The objective was to identify the microorganisms present on polyglycolic−acid braided suture material coated with triclosan used in the oral cavity and the in vitro evaluation of the bactericidal properties of triclosan−coated material.
Material and Methods. Material was collected from 11 patients subjected oral surgery. The first material was nonabsorbable monofilamen the second triclosan−coated multifilament. Both threads were sutured simultaneously to the same wound. The suture materials were excised 7 days after surgery and placed onto transport medium plates. Identification was carried out with commercial API tests. Most of the isolated strains were classified as Streptococcus. In vitro tests were performed in two stages. The first test was carried out with the coated materials placed directly onto plates inoculated with microbes isolated from the patients. In the second test the sutures were first rinsed for 24 h in isotonic salt solution. Zones of growth inhibition were evaluated after 48 h.
Results. There was no significant difference in colony count and species of microbes isolated from triclosan−coated and uncoated material. The growth inhibition of Streptococci did not exceed 2 mm and was observed only with unrinsed triclosan−coated material. A larger zone of inhibition of up to 20 mm was noted only with the control strain E. coli ATCC 25922 with both unrinsed and rinsed material.
Conclusion. In the absence of demonstrated differences in colonization between sutures coated with triclosan and regular multifilament suture and their bactericidal properties, the choice for suture material should be its convenience for the surgeon rather than its ability for bacterial adherence.
Streszczenie
Cel pracy. Identyfikacja mikroorganizmów obecnych na wielowłókienkowych niciach chirurgicznych pokrywanych triklosanem, zastosowanych w jamie ustnej, oraz określenie właściwości przeciwbakteryjnych tego typu wyrobu.
Materiał i metody. Materiał pobrano od 11 pacjentów poddanych zabiegom z zakresu chirurgii stomatologicznej. Rany pooperacyjne zeszyto jednoczasowo monofilamentem i nicią plecioną, pokrytą triklosanem. Identyfikacji mikroorganizmów dokonano za pomocą testów API. Badania in vitro przeprowadzono na niciach bezpośrednio pobieranych od pacjentów, a w teście hamowania wzrostu również na płukanych przez 24 h w soli fizjologicznej.
Wyniki. Nie stwierdzono istotnych różnic w rodzaju i liczbie drobnoustrojów izolowanych z nici pokrytych i niepokrytych triklosanem. Strefa zahamowania wzrostu dla szczepu Streptococci nie przekroczyła 2 mm i zaobserwowano ją tylko dla nici niepoddanych płukaniu. Strefę > 20 mm stwierdzono tylko dla testowego szczepu Escherichia coli.
Wnioski. Brak różnic w kolonizacji nici chirurgicznych oraz ich mała skuteczność przeciwbakteryjna pozwala stwierdzić, że podstawowym czynnikiem podczas wyboru materiału szewnego w dalszym ciągu pozostają jego właściwości mechaniczne i kryteria ekonomiczne, a nie potencjalne oddziaływanie przeciwbakteryjne.
Key words
surgical suture material, triclosan, bacterial colonization
Słowa kluczowe
nici chirurgiczne, triklosan, kolonizacja bakterii
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