Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
Scopus CiteScore – 3.4 (CiteScore Tracker 3.7)
Index Copernicus  – 161.11; MNiSW – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2008, vol. 17, nr 2, March-April, p. 183–189

Publication type: original article

Language: English

Soluble Adhesion Molecules During a Single Dialysis Session in Children and Young Adults on Chronic Hemodialysis

Rozpuszczalne cząstki adhezyjne podczas pojedynczej sesji dializacyjnej u dzieci i młodych dorosłych przewlekle hemodializowanych

Kinga Musiał1,, Danuta Zwolińska1,, Krystyna Szprynger2,, Maria Szczepańska2,

1 Department of Pediatric Nephrology, Silesian Piasts University of Medicine in Wrocław, Poland

2 Department of Pediatrics, Clinic of Nephrology, Endocrinology and Metabolic Diseases of Childhood, Silesian School of Medicine, Zabrze, Poland

Abstract

Background. Immune system impairment in patients on hemodialysis (HD) may partly result from bioincompatibility of dialysis membranes.
Objectives. The aim of the study was to analyze the impact of a single dialysis session and the type of dialysis membrane on soluble(s) adhesion molecules, thus evaluating their value as biocompatibility markers.
Material and Methods. Serum sL−selectin and sVCAM−1 concentrations were assessed by ELISA. These parameters and the sL−selectin/sVCAM−1 ratio (L/V) were examined in children and young adults on maintenance HD: 14 with cuprophane (CU), 10 with vitamin E−modified cellulose (VE), and 8 with polysulfone (PS) membranes, as well as in 15 controls. Baseline laboratory test results, age, BMI, and time of therapy were also evaluated and regression analysis was performed.
Results. Linear correlations were found between sL−selectin, L/V, and the type of dialyzer before and after dialysis session. The correlation coefficient for the linear regression equation after HD was higher than before the session (sL−selectin: R = 0.6 vs. R = 0.5, L/V: R = 0.7 vs. R = 0.6) and was stronger for L/V. Linear correlation was found between sVCAM−1 and the membrane type after HD (R = 0.5).
Conclusion. The relationships between adhesion molecules and dialyzer type suggest that they may serve as markers of biocompatibility. The sL−selectin correlation, which suggests an impact of the membrane on leukocyte function, requires further investigation as one of the possible explanations for the increased incidence of infections in HD children.

Streszczenie

Wprowadzenie. Zaburzenie odporności, obserwowane u pacjentów hemodializowanych, może wynikać m.in. z zastosowania bioniezgodnych błon dializacyjnych.
Cel pracy. Analiza wpływu pojedynczej sesji hemodializy i typu błony dializacyjnej na stężenia rozpuszczalnych (s) cząstek adhezyjnych i ocena przydatności tych molekuł jako markerów biozgodności zastosowanych dializatorów.
Materiał i metody. Stężenia sL−selektyny i sVCAM−1 w surowicy oznaczano metodą ELISA. Powyższe parametry i współczynnik sL−selektyna/sVCAM−1 (L/V) oceniano u dzieci i młodych dorosłych przewlekle hemodializowanych: 14 pacjentów – z użyciem dializatorów kuprofanowych (CU), 10 – błon celulozowych pokrytych witaminą E, 8 – dializatorów polisulfonowych i w grupie kontrolnej 15 osób. Wykonano również podstawowe badania laboratoryjne, w analizie regresji uwzględniono także wiek pacjentów, BMI i czas terapii.
Wyniki. Wykazano liniową zależność między sL−selektyną, L/V i typem dializatora, zarówno przed, jak i po zabiegu hemodializy. Współczynnik korelacji dla równania regresji liniowej po HD był wyższy niż przed zabiegiem (sL−selektyna – R = 0.6 vs. R = 0.5; L/V – R = 0.7 vs. R = 0.6) i silniejszy w przypadku L/V. Liniową zależność między sVCAM−1 i typem dializatora wykazano po sesji HD (R = 0.5).
Wnioski. Zależności między cząstkami adhezyjnymi a typem dializatora wskazują, że parametry te mogą pełnić rolę markerów biozgodności. Korelacja obserwowana w przypadku sL−selektyny może sugerować wpływ błon dializacyjnych na funkcję leukocytów. Konieczne są dalsze badania tej molekuły w celu wyjaśnienia przyczyn zwiększonej podatności na infekcje pacjentów pediatrycznych leczonych hemodializami.

Key words

sL−selectin, sVCAM−1, immune system, chronic kidney disease

Słowa kluczowe

sL−selektyna, sVCAM−1, układ immunologiczny, przewlekła choroba nerek

References (18)

  1. Cheung AK, Mohammad SF, Leypoldt JK.: Adhesion molecules and artificial membranes. In: Adhesion molecules in health and disease. Eds.: Leendert CP, Issekutz TB, Marcel Dekker, New York 1997, 643–678.
  2. Walker RJ, Sutherland WH, De Jong SA.: Effect of changing from a cellulose acetate to a polysulphone dialysis membrane on protein oxidation and inflammation markers. Clin Nephrol 2004, 61, 198–206.
  3. Wu CC, Chen JS, Wu WM et al.: Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes. Nephrol Dial Transplant 2005, 20, 1134–1139.
  4. Soriano S, Martin−Malo A, Carracedo J, Ramirez R, Rodriguez M, Aljama P.: Lymphocyte apoptosis: role of uremia and permeability of dialysis membrane. Nephron 2005, 100, c71–c77.
  5. Carlos TM, Harlan JM: Leukocyte−endothelial adhesion molecules. Blood 1994, 84, 2068–2101.
  6. Kawabata K, Nagake Y, Shikata K, Makino H, Ota Z: The changes of Mac−1 and L−selectin expression on granulocytes and soluble L−selectin level during hemodialysis. Nephron 1996, 73, 573–579.
  7. Dou L, Brunet P, Dignat−George F, Sampol J, Berland Y: Effect of uremia and hemodialysis on soluble L−selectin and leukocyte surface CD11b and L−selectin. Am J Kidney Dis 1998, 31, 67–73.
  8. Mrowka C, Heintz B, Sieberth HG.: Is dialysis membrane type responsible for increased circulating adhesion molecules during chronic hemodialysis? Clin Nephrol 1999, 52, 312–321.
  9. Rabb H, Calderon E, Bittle PA, Ramirez G: Alterations in sICAM−1 and sVCAM−1 in hemodialysis patients. Am J Kidney Dis 1996, 27, 239–243.
  10. Bonomini M, Reale M, Santarelli P, Stuard S, Settefrati N, Albertazzi A: Serum levels of soluble adhesion molecules in chronic renal failure and dialysis patients. Nephron 1998, 79, 399–407.
  11. Reno F, Lombardi F, Cannas M: Polystyrene surface coated with vitamin E modulates human granulocyte adhesion and MMP−9 release. Biomol Eng 2004, 21, 73–80.
  12. Tsuruoka S, Kawaguchi A, Nishiki K et al.: Vitamin E−bonded hemodialyzer improves neutrophil function and oxidative stress in patients with end−stage renal failure. Am J Kidney Dis 2002, 39, 127–33.
  13. Mydlik M, Derzsiova K, Racz O, Sipulova A, Lovasova E, Molcanyiova A, Petrovicova J: Vitamin E−coated dialyzer and antioxidant defense parameters: three−month study. Semin Nephrol 2004, 24, 525–531.
  14. Himmelfarb J, Zaoui P, Hakim R, Holbrook D: Modulation of granulocyte LAM−1 and MAC−1 during dialysis – a prospective, randomized trial. Kidney Int 1992, 41, 388–395.
  15. Musiał K, Zwolińska D, Polak−Jonkisz D, Berny U, Szprynger K, Szczepańska M: Soluble adhesion molecules in children and young adults on hemodialysis. Pediatr Nephrol 2004, 19, 332–336.
  16. Hernandez MR, Galan AM, Cases A et al: Biocompatibility of cellulosic and synthetic membranes assessed by leukocyte activation. Am J Nephrol 2004, 24, 235–241.
  17. Dhondt A, Vanholder R, Glorieux G, Waterloos MA, De Smet R, Lesaffer G, Lameire N: Vitamin E−bonded cellulose membrane and hemodialysis bioincompatibility absence of an acute benefit on expression of leukocyte surface molecules. Am J Kidney Dis 2000, 36, 1140–1146.
  18. Grooteman MPC, Gritters M, Wauters IMPM et al.: Patient characteristics rather than the type of dialyser predict the variability of endothelial derived surface molecules in chronic haemodialysis patients. Nephrol Dial Transplant 2005, 20, 2751–2758.
© Wroclaw Medical University