Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2006, vol. 15, nr 2, March-April, p. 285–291

Publication type: original article

Language: English

Dynamics of Humoral Immunity Indices and of Haematological Variables in Rabbits Immunized with Chlamydophila psittaci

Dynamika wskaźników odporności humoralnej oraz wskaźników hematologicznych u królików immunizowanych Chlamydophila psittaci

Małgorzata Pawlikowska1,, Wiesław Deptuła1,

1 Department of Microbiology and Immunology, Faculty of Natural Sciences, University of Szczecin, Poland

Abstract

Introduction. The research of humoral immunity in infection or immunization with Chlamydophila psittaci in static models showed changes in parameters of this immunity.
Objectives. Evaluation of chosen parameters of humoral immunity in dynamic model in rabbits immunized with Chlamydophila psittaci.
Material and Methods. The studies were performed on rabbits divided into two groups of 10 animals each. The rabbits of first group were immunized with Chlamydophila psittaci – strain 6BC, and the animals of second group were control animals. Blood samples were examined 9 times every 7 days. The activity of myeloperoxidase in polymorphonuclear leukocytes (PMN), amount and activity of lysozyme, total amount of immunoglobulins (Ig), including those of IgG class were estimated in sera of rabbits immunized with Chlamydophila psittaci. Haemoglobin level, content of erythrocytes and leukocytes, blood differential count and titre of Chlamydia spp.− −specific antibodies were also established by complement−fixation technique.
Results. The results indicate that immunization of rabbits with the bacteria induces with a similar frequency increase or decrease in 2 out of 6 examined parameters of humoral immunity (amount and activity of lysozyme) and rather a decrease than increase in 3 out of 8 examined haematological variables (count of neutrophiles, basophiles and monocytes). The alterations, mainly those in immune parameters, could be noted 5 weeks before appearance of Chlamydia spp.−specific antibodies, which appeared on 49th–56th day of the experiment.
Conclusion. The alterations noted in parameters of humoral immunity, may turn to be useful in determining the condition of macro−organisms in contact with Chlamydophila psittaci.

Streszczenie

Wprowadzenie. Badania odporności humoralnej w zakażeniu lub immunizacji bakteriami Chlamydophila psittaci w układach statycznych wykazały zmiany wskaźników tej odporności.
Cel pracy. Ocena zmian wybranych parametrów odporności humoralnej w układzie dynamicznym u królików immunizowanych Chlamydophila psittaci.
Materiał i metody. Badania przeprowadzono na królikach podzielonych na dwie grupy, po 10 zwierząt każda. Króliki z pierwszej grupy immunizowano Chlamydophila psittaci – szczep 6BC, a zwierzęta z drugiej grupy były grupą kontrolną. Krew do badań pobierano 9−krotnie w odstępach 7−dniowych. Oznaczano aktywność mieloperoksydazy w komórkach polimorfonuklearnych (PMN) oraz ilość i aktywność lizozymu, ogólną ilość immunoglobulin (Ig), w tym Ig klasy G, w surowicy u królików immunizowanych Chlamydophila psittaci. Oznaczano także ilość hemoglobiny, liczbę erytrocytów i leukocytów, obraz jakościowy krwi oraz miano swoistych przeciwciał anty−Chlamydia sp.
Wyniki. Z opisanych badań wynika, że immunizacja królików tą bakterią powoduje wzrost i spadek występujący z podobną częstotliwością w 2 z 6 badanych wskaźników odporności humoralnej (ilość i aktywność lizozymu) oraz częściej spadek niż wzrost w 3 z 8 badanych wskaźników hematologicznych (liczba neutrofilów, bazofilów i monocytów). Zmiany, głównie wskaźników odpornościowych, stwierdzono 5 tygodni przed pojawieniem się swoistych przeciwciał anty−Chlamydia sp., które pojawiły się w 49.–56. dniu doświadczenia.
Wnioski. Zmiany wskaźników odporności humoralnej mogą być przydatne do określania stanu makroorganizmu po kontakcie z Chlamydophila psittaci.

Key words

Chlamydophila psittaci, rabbit, immune indices, haematological indices

Słowa kluczowe

Chlamydophila psittaci, królik, wskaźniki odpornościowe, wskaźniki hematologiczne

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