Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 2.1
5-Year Impact Factor – 2.2
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2006, vol. 15, nr 1, January-February, p. 121–125

Publication type: review article

Language: Polish

Paleomikrobiologia – nowa dziedzina nauki

Paleomicrobiology – a New Branch of Science

Jan Kołodyński1,, Stanisław Jankowski2,

1 Instytut Genetyki i Mikrobiologii UW we Wrocławiu

2 Katedra i Zakład Biologii i Parazytologii Lekarskiej AM we Wrocławiu


Molekularne techniki pozwalają na identyfikację kwasu nukleinowego drobnoustrojów chorobotwórczych, występującego w archeologicznych szczątkach ludzi i zwierząt. Przedstawione w pracy przykłady dowodzą, że współ− czesna nauka uzyskała możliwość wykrycia czynników etiologicznych niektórych chorób zakaźnych w dawno wymarłych populacjach.


Molecular techniques allow identification of a nucleic acid of infectious microorganisms present in fossil remains of men and animals. The cases presented prove that the contemporary science has acquired a possibility of extinguished populations.

Słowa kluczowe

dawne DNA, gruźlica, mumia, paleomikrobiologia

Key words

ancient DNA, tuberculosis, mummy, paleomicrobiology

References (39)

  1. Pääbo S: Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification. Proc Natl Acad Sci USA 1989, 86: 1939–1943.
  2. Marota I, Rollo F: Molecular paleontology. Cell Mol Life Sci 2002, 59, 97–111.
  3. Hőss M, Jaruga P, Zastawny TH, Dizdaroglu M, Pääbo S: DNA damage and DNA sequence retrieval from ancient tissues. Nucleic Acids Res 1996, 24, 1304–1307.
  4. Hofreiter M, Serre D, Poinar HN, Kuch M, Pääbo S: Ancient DNA. Nat Rev Gen 2001, 2, 353–359.
  5. Zink AR, Reischl U, Wolf H, Nerlich AG: Molecular analysis of ancient microbial infections. FEMS Microbiol Let 2002, 213, 141–147.
  6. Woodward SR, Weyand NJ, Bunnell M: DNA sequence from Cretaceous period bone fragments. Science 1994, 266, 1229–1232
  7. Cooper A, Poinar HN: Ancient DNA: do it right or not at all. Science 2000, 289, 1139.
  8. Poinar HN: The top 10 list: criteria of authenticity for DNA from ancient and forensic samples. Int Congress Ser 2003, 1239, 575–579.
  9. Donoghue HD, Spigelman M, Greenblatt CL, Lev−Maor G, Bar−Gal GK, Mathenson C, Vernon K, Nerlich AG, Zink AR: Tuberculosis: from prehistory to Robert Koch, as revealed by ancient DNA. Lancet Infect Dis 2004, 4, 584–592.
  10. Spigelman M, Lemma E: The use of the polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in ancient skeletons. Int J Osteoarcheol 1993, 3, 137–143.
  11. Salo WL, Aufderheide AC, Buikstra J, Holcomb TA: Identification of Mycobacterium tuberculosis DNA in a pre−Columbian Peruvian mummy. Proc Nat Acad Sci 1994, 91, 2091–2096.
  12. Crubezy E, Ludes B, Poveda JD, Clayton J, Crouau−Roy B, Montagnon D: Identification of Mycobacterium DNA in an Egyptian Pott’s disease of 5400 years old. CR Acad Sci III 1998, 321, 941–951.
  13. Zink AR, Haas CJ, Reischl U, Szeimies U, Nerlich AG: Molecular analysis of skeletal tuberculosis in an ancient Egyptian population. J Med Microbiol 2001, 50, 355–366.
  14. Zink AR, GrabnerW, Reischl U, Wolf H, Nerlich AG: Molecular study on human tuberculosis in three geographically distinct and time delineated populations from ancient Egypt. Epidemiol Infect 2003, 130, 239–249.
  15. Haas CJ, Zink A, Molnar E, Szeimies U, Reischl U, Marcsik A, Ardagna Y, Dutour O, Palfi G, Nerlich AG: Molecular evidence for different stages of tuberculosis in ancient bone samples from Hungary. Am J Phys Anthropol 2000, 113, 293–304.
  16. Faerman M, Jankauskas R: Paleopathological and molecular evidence of human bone tuberculosis in Iron Age Lithuania. Anthropol Anz 2000, 58, 57–62.
  17. Mays S, Taylor GM, Legge AJ, Young DB, Turner−Walker G: Paleopathological and biomolecular study of tuberculosis in an medieval skeletal collection from England. Am J Phys Anthropol 2001, 114, 298–311.
  18. Gomez i Prat J, de Souza SMFM: Prehistoric tuberculosis in America: adding comments to a literature review. Mem Inst Oswaldo Cruz, Rio de Janeiro 2003, 98 (Suppl.1), 151–159.
  19. Kamerbeek J, Schouls L, Kolk A, Agterveld M, van Soolingen D, Kuijper S, Bunschoten A, Molhuizen H, Shaw R, Goyal M, van Embden JDA: Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 1997, 35, 907–914.
  20. Rothschild BM, Martin LD, Lev G, Bercovier H, Bar−Gal GK, Greenblatt C, Donoghue H, Spigelman M, Brittain D: Mycobacterium tuberculosis complex DNA from an extinct bison dated 17 000 years before the present. Clin Infect Dis 2001, 33, 305–311.
  21. Mays S, Taylor GM, Legge AJ, Young DB, Turner−Walker G: Paleopathological and biomolecular study of tuberculosis in a medieval skeletal collection from England. Am J Phys Anthropol 2001, 114, 298–311.
  22. Kapur V, Whittam TS, Musser JM: Is Mycobacterium tuberculosis 15 000 years old? J Inf Dis 1994, 170, 1348–1349.
  23. Flether HA, Donoghue HD, Holton J, Pap I, Spigelman M: Widespread occurrence of Mycobacterium tuberculosis DNA from 18th–19th century Hungarians. Am J Phys Anthropol 2003, 120, 144–152.
  24. Rafi A, Spigelman M, Stanford J, Lemma E, Donoghue H, Zias J: DNA of Mycobacterium leprae detected in ancient bone. Int J Osteoarchaeol 1994, 4, 287–290.
  25. Haas CJ, Zink A, Palfi G, Szeimies U, Nerlich AG: Detection of leprosy in ancient human skeletal remains by molecular identification of Mycobacterium leprae. Am J Clin Pathol 2000, 114, 428–436.
  26. Taylor MG, Widdison S, Brown IN, Young D, Molleson T: A mediaeval case of lepromatous leprosy from 13–14th century Orkney, Scotland. J Archeolog Sci 2000, 27, 1133–1138.
  27. Drancourt M, Aboudharam G, Signoli M, Dutour O, Raoult D: Detection of 400−year−old Yersinia pestis DNAin human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci 2000, 95, 12637–12640.
  28. Raoult D, Aboudharam G, Crubezy E, Larrouy G, Ludes B, Drancourt M: Molecular identification by “suicide PCR” of Yersinia pestis as the agent of Medieval Black Death. Proc Natl Acad Sci 2000, 97, 12800–12803.
  29. Wood J, de Witte−Avina S: Was the Black Death yersinial plague? Lancet Infect Dis 2003, 3, 327.
  30. Prentice MB, Gilbert T, Cooper A:Was the Black Death caused by Yersinia pestis? Lancet Infect Dis 2004, 4, 72.
  31. Gilbert MTP, Cuccui J, White W, Lynnerup N, Titball RW, Cooper A, Prentice MB: Absence of Yersinia pestisspecific DNA in human teeth from five European excavations of putative plague victims. Microbiol 2004, 150, 341–354.
  32. Zink A, Reischl U, Wolf H, Nerlich A: Molecular evidence for bacteremia by gastrointestinal pathogenic bacteria in an infant mummy from ancient Egypt. Arch Pathol Lab Med 2000, 124, 1614–1618.
  33. Zink A, Reischl U, Wolf H, Nerlich AG, Miller RL: Corynebacterium in ancient Egypt. Med Hist 2001, 45, 267–272.
  34. Taubenberger JK, Reid AH, Krafft AE, Bijwaard KE, Fanning TG: Initial characterization of the 1918 “Spanish” influenza virus. Science 1997, 275, 1793–1796.
  35. Reid AH, Fanning TG, Hultin JV, Taubenberger JK: Origin and evolution of the 1918 “Spanish” influenza virus hemagglutinin gene. Proc Natl Acad Sci 1999, 96, 1651–1656.
  36. Basler CF, Reid AH, Dybing JK, Janczewski TA, Fanning TG, Zheng H, Salvatore M, Perdue ML, Swayne DE, Garcia−Sastre A, Palese P, Taubenberger JK: Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses bearing the 1918 NS genes. Proc Natl Acad Sci 2001, 98, 2746–2751.
  37. Li HC, Fujiyoshi T, Lou H, Yashiki S, Sonoda S, Cartier L, Nunez I, Munoz I, Horai S, Tajima K: The presence of ancient human T−cell lymphotropic virus type I provirus DNA in an Andean mummy. Nat Med 1999, 5, 1428–1432.
  38. Feoli−Fonsenca JC, Oligny LL, Filion M, Simard P, Russo PA, Yotov WV: A putative novel human papillomavirus identified by PCR−DS. Biochem Biophys Res Commun 1998, 250, 63–67.
  39. Araujo A, Ferreira LF: Paleoparasitology and the antiquity of human host−parasite relationships. Mem Inst Oswaldo Cruz 2000, 95 (Suppl. 1), 89–93.