Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 4, April, p. 423–429

doi: 10.17219/acem/116750

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Single nucleotide polymorphisms of SCN5A and SCN10A genes increase the risk of ventricular arrhythmias during myocardial infarction

Hajer Foddha1,2,A,B,C,D,F, Nadia Bouzidi1,B, Abdelhak Foddha3,A,B,C, Saoussen Chouchene4,B, Rahma Touhami1,2,B, Nadia Leban1,E, Mohamed Faouzi Maatoug3,B, Habib Gamra3,B, Salima Ferchichi1,B, Jemni Ben Chibani1,E, Amel Haj Khelil1,2,C,D,E,F

1 Laboratory of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Monastir, Tunisia

2 Higher Institute of Biotechnology, University of Monastir, Tunisia

3 Department of Cardiology, Fattouma Bourguiba University Hospital of Monastir, Tunisia

4 Department of Hematology, Fattouma Bourguiba University Hospital of Monastir, Tunisia

Abstract

Background. Coronary artery disease (CAD) and its ultimate consequence − myocardial infarction (MI) − are major causes of sudden cardiac death (SCD). Previous studies have demonstrated the role of genetic polymorphisms in the risk of SCD and ventricular arrhythmia (VA) during MI.
Objectives. To investigate the association between single nucleotide polymorphisms (SNPs) of genes implicated in congenital cardiac arrhythmias and the risk of developing VA in the context of MI.
Material and Methods. We performed a case–control study in which we genotyped 4 SNPs (rs11708996, rs10428132, rs9388451, and rs2200733) in 469 subjects using amplification refractory mutation system (ARMS) and a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). These SNPs are located in the SCN5A, SCN10A, HEY2, and PITX2 genes, respectively. We first compared 70 patients who had developed VA in the context of MI with 141 healthy controls; next, we compared VA patients with 258 MI patients who did not develop VA during a 1-year follow up. The statistical analyses were adjusted for sex and age.
Results. Compared to the controls, 2 polymorphisms were significantly associated with the development of VA during MI, located in SCN5A rs11708996 (p = 0.001) and SCN10A rs10428132 (p = 0.001). Similar results were found when comparing VA cases with patients without VA. No associations of HEY2 and PITX2 polymorphisms were observed.
Conclusion. Our results suggest that the rs11708996 and rs10428132 polymorphisms of the SCN5A and SCN10A genes may contribute to an elevated risk of developing VA in the context of MI. The associated alleles or genotypes may be used to predict the risk, and thus prevent eventual SCD.

Key words

single nucleotide polymorphism, myocardial infarction, ventricular arrhythmia, sudden cardiac death

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