Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2020, vol. 29, nr 2, February, p. 215–224

doi: 10.17219/acem/115086

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Microbiological, antioxidant and lipoxygenase-1 inhibitory activities of fruit extracts of chosen Rosaceae family species

Andrzej B. Hendrich1,A,B,D,E,F, Paulina Strugała2,B,C,E,F, Anna Dudra2,B, Alicja Z. Kucharska3,B,D,E, Anna Sokół-Łętowska3,B,D,E, Dorota Wojnicz1,A,B,D,E,F, Agnieszka Cisowska1,B,D, Zbigniew Sroka4,B, Janina Gabrielska2,A,D,E,F

1 Department of Biology and Medical Parasitology, Wroclaw Medical University, Poland

2 Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, Poland

3 Department of Fruit, Vegetable and Cereal Technology, Wroclaw University of Environmental and Life Sciences, Poland

4 Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Poland

Abstract

Background. Extracts from the Rosaceae family fruits are rich in natural, biologically active polyphenols, but their antibacterial properties are still poorly understood. Therefore, we focused our research on their activity against uropathogenic Escherichia coli strains. This research also concerned the proof of their ability to reduce oxidative stress and modulate the activity of lipoxygenase-1 (LOX-1). It is well-known that plants represent a source of bioactive compounds whose antioxidant activity may be useful in protecting against oxidative damage in cells, which have been linked to the pathogenesis of many oxidative diseases.
Objectives. The study determined the biological activity of methanol (ME) and water (WE) extracts rich in polyphenols from the hawthorn (Crataegus monogyna Jacq.), dog rose (Rosa canina L.), quince (Cydonia oblonga Mill.), and Japanese quince (Chaenomeles speciosa (Sweet) Nakai).
Material and Methods. The antioxidant capacity was evaluated using 1,1diphenyl-2-picrylhydrazyl (DPPH▪) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+▪) radical scavenging methods. The inhibition of liposome membrane oxidation was studied using the thiobarbituric acid reactive substances assay. Lipoxygenase-1 inhibitory activity was measured using the spectrophotometric method. Bacterial growth was determined by evaluating the number of colony forming units per milliliter (CFU/mL). Hydrophobicity was established with salt aggregation hydrophobicity test (SAT). Swimming and swarming motilities were evaluated using soft-agar plates. Production of curli fimbriae was estimated on CFA agar. The P fimbriae were detected using the hemagglutination of erythrocytes. Adhesion of bacteria to human uroepithelial cells was assessed. The amount of biofilm was determined spectrophotometrically.
Results. We showed that most of these extracts are effective antioxidants and free radical scavengers, possess reasonable potential anti-inflammatory activity, reduce the adhesion of E. coli to uroepithelial cells, and reduce the ability of these bacteria to form biofilm.
Conclusion. The extracts examined, showing very promising biological properties, seem to be able to join the list of substances that can be used as dietary supplements aimed at preventing, for example, urinary tract infections, or as support of drug treatment in many diseases.

Key words

Rosaceae fruit extracts, antimicrobial, antioxidant, lipoxygenase-1 inhibition

References (35)

  1. González de Llano D, Liu H, Khoo C, Moreno-Arribas MV, Barto­lomé B. Some new findings regarding the antiadhesive activity of cranberry phenolic compounds and their microbial-derived metabolites against uropathogenic bacteria. J Agric Food Chem. 2019;67(8):2166−2174.
  2. Kylli P, Nohynek L, Puupponen-Pimiä R, et al. Lingonberry (Vaccinium vitis-idaea) and European cranberry (Vaccinium microcarpon) proanthocyanidins: Isolation, identification, and bioactivities. J Agric Food Chem. 2011;59(7):3373–3384.
  3. Moyer RA, Hummer KE, Finn CE, Frei B, Wrolstad RE. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, rubus, and ribes. J Agric Food Chem. 2002;50(3):519–525.
  4. Blumberg JB, Camesano TA, Cassidy A, et al. Cranberries and their bioactive constituents in human. Health Adv Nutr. 2013;4(6):618–632.
  5. Kim HW, Chung DH, Kim SA, Rhee MS. Synergistic cranberry juice combinations with natural‐borne antimicrobials for the eradication of uropathogenic Escherichia coli biofilm within a short time. Lett Appl Microbiol. 2019;68(4):321–328.
  6. Kirkeskov B, Christensen R, Bügel S, et al. The effects of rose hip (Rosa canina) on plasma antioxidative activity and C-reactive protein in patients with rheumatoid arthritis and normal controls: A prospective cohort study. Phytomedicine. 2011;18(11):953–958.
  7. Edwards JE, Brown PN, Talent N, Dickinson TA, Shipley PR. A review of the chemistry of the genus Crataegus. Phytochemistry. 2012;79:5–26.
  8. Oliveira AP, Pereira JA, Andrade PB, Valentão P, Seabra RM, Silva BM. Phenolic profile of Cydonia oblonga Miller leaves. J Agric Food Chem. 2007;55(19):7926–7930.
  9. Sroka Z, Rządkowska-Bodalska H, Mażol I. Antioxidative effect of extracts from Erodium cicutarium L. Z Naturforsch C J Biosci. 1994;49(11–12):881–884.
  10. Strugała P, Loi S, Bażanów B, et al. A comprehensive study on the biological activity of elderberry extract and cyanidin 3-O-glucoside and their interactions with membranes and human serum albumin. Molecules. 2018;23(10). doi:10.3390/molecules23102566.
  11. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–1237.
  12. Axelrod B, Cheesbrough TM, Laakso S. Lipoxygenases in soybeans. Method Enzymol. 1981;71:441–451.
  13. Hidalgo G, Chan M, Tufenkji N. Inhibition of Escherichia coli CFT073 fliC expression and motility by cranberry materials. Appl Environ Microbiol. 2011;77(19):6852–6857.
  14. Sanchez-Torres V, Hu H, Wood TK. GGDEF proteins YeaI, YedQ, and YfiN reduce early biofilm formation and swimming motility in Escherichia coli. Appl Microbiol Biotechnol. 2011;90(2):651–658.
  15. Siegfried L, Kmetová M, Puzová H, Molokáčová M, Filka J. Virulence-associated factors in Escherichia coli strains isolated from children with urinary tract infections. J Med Microbiol. 1994;41(2):127–132.
  16. Rosser T, Dransfield T, Allison L, et al. Pathogenic potential of emergent sorbitol-fermenting Escherichia coli O157: NM. Infect Immun. 2008;76(12):5598–5607.
  17. Latham RH, Stamm WE. Role of fimbriated Escherichia coli in urinary tract infections in adult women: Correlation and localization studies. J Infect Dis. 1984;149(6):835–840.
  18. Wojnicz D, Sycz Z, Walkowski S, et al. Study on the influence of cranberry extract Żuravit S-O-S® on the properties of uropathogenic Escherichia coli strains, their ability to form biofilm and its antioxidant properties. Phytomedicine. 2012;19(6):506–514.
  19. O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signaling pathways: A genetic analysis. Mol Microbiol. 1998;28(3):449–461.
  20. Stepanović S, Cirković I, Ranin L, Svabić-Vlahović M. Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Lett Appl Microbiol. 2004;38(5):428–432.
  21. Grace MH, Esposito D, Dunlap KL, Lila MA. Comparative analysis of phenolic content and profile, antioxidant capacity, and anti-inflammatory bioactivity in wild Alaskan and commercial Vaccinium berries. J Agric Food Chem. 2014;62(18):4007–4017.
  22. Paredes-López O, Cervantes-Ceja ML, Vigna-Pérez M, Hernández-Pérez T. Berries: Improving human health and healthy aging, and promoting quality life: A review. Plant Foods Hum Nutr. 2010;65(3):299–308.
  23. Silva BM, Andrade PB, Ferreres F, Domingues AL, Seabra RM, Ferreira A. Phenolic profile of quince fruit (Cydonia oblonga Miller) (pulp and peel). J Agr Food Chem. 2002;50(16):4615–4618.
  24. Du H, Wu J, Li H, et al. Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment. Food Chem. 2013;141(4):4260–4268.
  25. Wenzig EM, Widowitz U, Kunert O, et al. Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations. Phytomedicine. 2008;15(10):826–835.
  26. Strugała P, Gładkowski W, Kucharska AZ, Sokół-Łętowska A, Gabriel­ska J. Antioxidant activity and anti-inflammatory effect of fruit extracts from blackcurrant, chokeberry, hawthorn, and rosehip, and their mixture with linseed oil on a model lipid membrane. Eur J Lipid Sci Technol. 2016;118(3):461–474.
  27. Tang Y, Yu X, Mi M, Zhao J, Wang J, Zhang YT. Antioxidative property and antiatherosclerotic effects of the powder processed from Chaenomelas speciosa in apoe−\− mice. J Food Biochem. 2010;34(3):535–548.
  28. Zhang SY, Han LY, Zhang H, Xin HL. Chaenomeles speciosa: A review of chemistry and pharmacology. Biomed Rep. 2014;2(1):12–18.
  29. Watychowicz K, Janda K, Jakubczyk K, Wolska J. Chaenomeles: Health promoting benefits. Rocz Panstw Zakl Hig. 2017;68(3):217–227.
  30. Ajila CM, Naidu KA, Bhat SG, Prasada Rao U. Bioactive compounds and antioxidant potential of mango peel extract. Food Chem. 2007;105(3):982–988.
  31. Chen CH, Chan HC, Chu YT, et al. Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase. Molecules. 2009;14(8):2947–2958.
  32. Tumbas VT, Canadanović-Brunet JM, Cetojević-Simin DD, Cetković GS, Ethilas SM, Gille L. Effect of rosehip (Rosa canina L.) phytochemicals on stable free radicals and human cancer cells. J Sci Food Agric. 2012;92(6):1273–1281.
  33. Patel S. Rose hip as an underutilized functional food: Evidence-based review. Trends Food Sci Technol. 2017;63:29–38.
  34. Cisowska A, Wojnicz D, Hendrich AB. Anthocyanins as antimicrobial agents of natural plant origin. Nat Prod Commun. 2011;6(1):149–156.
  35. Packiavathy IA, Priya S, Pandian SK, Ravi AV. Inhibition of biofilm development of uropathogens by curcumin: An anti-quorum sensing agent from Curcuma longa. Food Chem. 2014;148:453–460.
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