Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 1, January, p. 75–83

doi: 10.17219/acem/76160

Publication type: original article

Language: English

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An initial evaluation of cytotoxicity, genotoxicity and antibacterial effectiveness of a disinfection liquid containing silver nanoparticles alone and combined with a glass-ionomer cement and dentin bonding systems

Alicja Porenczuk1,A,B,C,D, Anna Grzeczkowicz2,A,B, Izabela Maciejewska3,C,D,E,F, Marlena Gołaś4,D, Katarzyna Piskorska4,D, Adam Kolenda5,C,D, Dariusz Gozdowski6,C, Ewa Kopeć-Swoboda4,E,F, Ludomira Granicka2,E,F, Dorota Olczak-Kowalczyk1,E,F

1 Department of Pediatric Dentistry, Medical University of Warsaw, Poland

2 The Maciej Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warszawa, Poland

3 Department of Molecular Medicine, Medical University of Gdańsk, Poland

4 Department of Medical Microbiology, Medical University of Warsaw, Poland

5 Department of Prosthetic Dentistry, Medical University of Warsaw, Poland

6 Department of Experimental Design and Bioinformatics, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Poland

Abstract

Background. Bacterial reinfection of dental cavities remains an unsolved clinical problem. The search for methods enabling the limitation of the bacterial factor has become the fundamental goal of the dental materials research. Silver nanoparticles (AgNPs) are used as disinfection agents. An incomplete polymerization of the polymer resins combined with AgNPs, along with the increase of the release of the unbound monomers, have been found.
Objectives. The aim of this study was to evaluate the vitality of the human dental pulp stem cells (DPSCs) in response to a disinfection agent containing silver and gold nanoparticles (NPs), different bonding systems, glass-ionomer cement (GIC), and their combinations with the disinfection agent. Also, the influence of these materials both on the secretory function of DPSCs and on their antibacterial properties was established.
Material and Methods. Cytotoxicity (MTT assay) and genotoxicity (enzyme-linked immunosorbent assay – ELISA) assays were used in the study. Antibacterial features were assessed with the optical density (OD) measurement of the bacteria (Streptococcus mutans, Streptococcus salivarius and Lactobacillus acidophilus) kept in dental materials.
Results. The disinfection liquid proved to be biocompatible. However, it relevantly interfered with the total-etch bonding system in terms of vitality, which may have serious clinical implications. Its combination with the self-etching system was biocompatible, yet it impaired the antibacterial action of the system. An enhancement of antibacterial action of GIC with AgNPs was found.
Conclusion. The disinfection liquid and GIC were biocompatible toward the DPSCs in terms of cytotoxicity and genotoxicity. Simultaneous usage of AgNPs with other dental materials did not affect the biocompatibility of the used materials. The disinfection liquid and GIC acted as antibacterial agents against all studied bacteria species. Used together with GIC and the total-etch bonding system, the disinfection liquid seemed to be efficient toward bacteria, yet it relevantly impaired the antibacterial action of self-etching systems.

Key words

cytotoxicity, silver nanoparticles, glass-ionomer cement, antibacterial properties, adhesives

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