Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 166.39
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 6, November-December, p. 1249–1254

doi: 10.17219/acem/65808

Publication type: original article

Language: English

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Normal Values for Heart Electrophysiology Parameters of Healthy Swine Determined on Electrophysiology Study

Agnieszka Noszczyk-Nowak1,A,B,C,D,F, Alicja Cepiel1,B,C,F, Adrian Janiszewski2,B,E,F, Robert Pasławski3,B,F, Jacek Gajek4,A,E,F, Urszula Pasławska1,B,E,F, Józef Nicpoń1,3,E,F

1 Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Poland

2 Center for Experimental Diagnostics and Biomedical Innovations, Wrocław, Poland

3 DEMONSTRATOR+, Administrative and Financial Office at the Wrocław University of Environmental and Life Sciences, Poland

4 Department of Cardiology, Wroclaw Medical University, Poland

Abstract

Background. Swine are a well-recognized animal model for human cardiovascular diseases. Despite the widespread use of porcine model in experimental electrophysiology, still no reference values for intracardiac electrical activity and conduction parameters determined during an invasive electrophysiology study (EPS) have been developed in this species thus far.
Objectives. The aim of the study was to develop a set of normal values for intracardiac electrical activity and conduction parameters determined during an invasive EPS of swine.
Material and Methods. The study included 36 healthy domestic swine (24–40 kg body weight). EPS was performed under a general anesthesia with midazolam, propofol and isoflurane. The reference values for intracardiac electrical activity and conduction parameters were calculated as arithmetic means ± 2 standard deviations.
Results. The reference values were determined for AH, HV and PA intervals, interatrial conduction time at its own and imposed rhythm, sinus node recovery time (SNRT), corrected sinus node recovery time (CSNRT), anterograde and retrograde Wenckebach points, atrial, atrioventricular node and ventricular refractory periods. No significant correlations were found between body weight and heart rate of the examined pigs and their electrophysiological parameters.
Conclusion. The hereby presented reference values can be helpful in comparing the results of various studies, as well as in more accurately estimating the values of electrophysiological parameters that can be expected in a given experiment.

Key words

heart, reference values, swine, electrophysiology study

References (27)

  1. Link MS, Wang PJ, Pandian NG, Bharati S, Udelson JE, Lee MY, Vecchiotti MA, VanderBrink BA, Mirra G, Maron BJ, Estes NA: An experimental model of sudden death due to low-energy chest-wall impact (commotio cordis). N Engl J Med 1998, 338, 1805–1811.
  2. Bergen WG, Mersmann HJ: Comparative aspects of lipid metabolism: Impact on contemporary research and use of animal models. J Nutr 2005, 135, 2499–2502.
  3. Alsheikh-Ali AA, Madias C, Supran S, Link MS: Marked variability in susceptibility to ventricular fibrillation in an experimental commotio cordis model. Circulation 2010, 122, 2499–2504.
  4. Pasławska U, Gajek J, Kiczak L, Noszczyk-Nowak A, Skrzypczak P, Bania J, Tomaszek A, Zacharski M, Sambor I, Dzięgiel P, Zyśko D, Banasiak W, Ponikowski P: Development of a partial model of chronic tachycardia – induced cardiomyopathy. Int J Cardiol 2011, 153, 36–41.
  5. Noszczyk-Nowak A, Nicpon J, Nowak M, Slawuta P: Preliminary reference values for electrocardiography, echocardiography and myocardial morphometry in the European brown hare (Lepus europaeus). Acta Vet Scand 2009, 51, 1751–0147.
  6. Soloviev MV, Hamlin RL, Shellhammer LJ, Barrett RM, Wally RA, Birchmeier PA, Schaefer GJ: Variations in hemodynamic parameters and ECG in healthy, conscious, freely moving telemetrized beagle dogs. Cardiovasc Toxicol 2006, 6, 51–62.
  7. Moise NS, Dietze AE, Mezza LE, Strickland D, Erb HN, Edwards NJ: Echocardiography, electrocardiography, and radiography of cats with dilatation cardiomyopathy, hypertrophic cardiomyopathy, and hyperthyroidism. Am J Vet Res 1986, 47, 1476–1486.
  8. Paslawska U, Noszczyk-Nowak A, Paslawski R, Janiszewski A, Kiczak L, Zysko D, Nicpon J, Jankowska EA, Szuba A, Ponikowski P: Normal electrocardiographic and echocardiographic (M-mode and two-dimensional) values in Polish Landrace pigs. Acta Vet Scand 2014, 56, 14–54.
  9. Bonagura JD, O’Grady MR, Herring DS: Echocardiography. Principles of interpretation. Vet Clin North Am Small Anim Pract 1985, 15, 1177–1194.
  10. Hallowell GD, Potter TJ, Bowen IM: Methods and normal values for echocardiography in adult dairy cattle. J Vet Cardiol 2007, 9, 91–98.
  11. Vastenburg M, Boroffka S, Schoemaker N: Echocardiographic measurements in clinically healthy ferrets anaesthetised with isoflurane. Vet Radiol Ultrasound 2004, 45, 228–232.
  12. Moses BL, Ross JN: M-mode echocardiographic values in sheep. Am J Vet Res 1987, 48, 1313–1318.
  13. Sleeper MM, Gaughan JM, Gleason CR, Burkett DE: Echocardiographic reference ranges for sedated healthy cynomolgus monkeys (Macaca fascicularis). J Am Assoc Lab Anim Sci 2008, 47, 22–25.
  14. Salemi VM, Bilate AM, Ramires FJ, Picard MH, Gregio DM, Kalil J, Neto EC, Mady C: Reference values from M-mode and Doppler echocardiography for normal Syrian hamsters. Eur J Echocardiogr 2005, 6, 41–46.
  15. Stypmann J, Engelen MA, Troatz C, Rothenburger M, Eckardt L, Tiemann K: Echocardiographic assessment of global left ventricular function in mice. Lab Anim 2009, 43, 127–137.
  16. Stein AB, Tiwari S, Thomas P, Hunt G, Levent C, Stoddard MF, Tang XL, Bolli R, Dawn B: Effects of anesthesia on echocardiographic assessment of left ventricular structure and function in rats. Basic Res Cardiol 2007, 102, 28–41.
  17. Chauvin M, Shah DC, Haı¨ssaguerre M, Marcellin L, Brechenmacher C: The anatomic basis of connections between the coronary sinus musculature and the left atrium in humans. Circulation 2000, 101, 647–652.
  18. Indrayan A: Medical Biostatistics. Chapman and Hall/CRC, Boca Raton 2012.
  19. Scanavacca MI, Venancio AC, Pisani CF, Lara S, Hachul D, Darrieux F, Hardy C, Paola E, Aiello VD, Mahapatra S, Sosa E: Percutaneous transatrial access to the pericardial space for epicardial mapping and ablation. Circ Arrhythm Electrophysiol 2011, 4, 331–336.
  20. Neven K, van Driel V, van Wessel H, van Es R, Doevendans PA, Wittkampf F: Epicardial linear electroporation ablation and lesion size. Heart Rhythm 2014, 11, 1465–1470.
  21. Kumar K, Nearing BD, Carvas M, Nascimento BC, Acar M, Belardinelli L, Verrier RL: Ranolazine exerts potent effects on atrial electrical properties and abbreviates atrial fibrillation duration in the intact porcine heart. J Cardiovasc Electrophysiol 2009, 20, 796–802.
  22. Zaballos M, Jimeno C, Almendral J, Atienza F, Patino D, Valdes E, Navia J, Anadon MJ: Cardiac electrophysiological effects of remifentanil: study in a closed-chest porcine model. Br J Anaesth 2009, 103, 191–198.
  23. Bechard J, Gibson JK, Killingsworth CR, Wheeler JJ, Schneidkraut MJ, Huang J, Ideker RE, McAfee DA: Vernakalant selectively prolongs atrial refractoriness with no effect on ventricular refractoriness or defibrillation threshold in pigs. J Cardiovasc Pharmacol 2011, 57, 302–307.
  24. Pires LA, Huang SK, Wagshal AB, Kulkarni RS: Electrophysiological effects of propofol on the normal cardiac conduction system. Cardiology 1996, 87, 319–324.
  25. Jones DJ, Stehling LC, Zauder HL: Cardiovascular responses to diazepam and midazolam maleate in the dog. Anesthesiology 1979, 51, 430–434.
  26. Carvas M, Nascimento BC, Acar M, Nearing BD, Belardinelli L, Verrier RL: Intrapericardial ranolazine prolongs atrial refractory period and markedly reduces atrial fibrillation inducibility in the intact porcine heart. J Cardiovasc Pharmacol 2010, 55, 286–291.
  27. Noszczyk-Nowak A: Electrophysiological study of the heart swine during experimental hyperthyroxinemia. Medycyna Weter 2007, 63, 1242–1246.