Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2016, vol. 25, nr 3, May-June, p. 465–470

doi: 10.17219/acem/38842

Publication type: original article

Language: English

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Alteration in Methylation Pattern of Retinoblastoma 1 Gene Promotor Region in Intestinal Metaplasia with or without Helicobacter pylori and Gastric Cancer Patients

Seda Orenay Boyacioglu1,A,B,C,D,E,F, Elmas Kasap2,A,B,C,D, Hakan Yuceyar2,A,B, Mehmet Korkmaz3,A,C,D

1 Department of Medical Genetics, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey

2 Department of Gastroenterology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey

3 Department of Medical Biology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey

Abstract

Background. Helicobacter pylori, intestinal metaplasia (IM), and gene methylation play important roles in gastric carcinogenesis. However, the association among H. pylori infection, IM, gastric cancer (GC), and gene methylation is not fully understood. Cell cycle control involving retinoblastoma 1 (RB1) gene is one of the main regulatory pathways reported to be altered in gastric carcinogenesis.
Objectives. The purpose of this research is to assess the methylation status of RB1 gene in GC and IM with or without H. pylori infection, and to discuss the possible role of H. pylori-induced RB1 gene methylation in the mechanism of gastric carcinogenesis.
Material and Methods. The methylation profile of RB1 gene was analyzed by sodium bisulfite modification and methylation-specific PCR in GC (n = 24), IM patients with H. pylori positive (n = 20) and negative (n = 20), and control subjects (n = 20).
Results. According to methylation levels in RB1 gene; the high correlation values were detected between H. pylori positive-IM group and GC group, and between H. pylori positive-IM and H. pylori negative-IM groups (p < 0.05). No correlations between H. pylori negative-IM and GC groups and between GC and control groups were detected in methylation status of RB1 gene.
Conclusion. High methylation levels in RB1 gene in H. pylori positive individuals may suggest an elevated risk of gastric cancer occurrence.

Key words

gastric cancer, Helicobacter pylori, intestinal metaplasia, methylation, retinoblastoma 1

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