Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 4, July-August, p. 637–641

doi: 10.17219/acem/22364

Publication type: original article

Language: English

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Experimental Esophagitis Model Preventing Tracheal Aspiration

Hasan A. Kiraz1,A,E, Mesut Erbas1,A,D, Adem Kucuk2,B,C, Naci Topaloglu3,B,E, Havva Erdem4,B,F, Hasan Sahin1,D,E, Huseyin Toman1,E,F, Aslı Kiraz5,E,F

1 Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

2 Department of Pediatric Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

3 Department of Pediatrics, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

4 Department of Pathology, Faculty of Medicine, Duzce University, Duzce, Turkey

5 Department of Medical Microbiology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Abstract

Background. Corrosive esophagitis injuries are a serious clinical problem with many agents used for diagnosis and treatment. Experimental esophagus burn models use a method described mainly in studies by Gehanno and Guedon, and modified by Liu and Richardson.
Objectives. The aim of this study was to describe a new esophagitis model that prevents tracheal aspiration.
Material and Methods. In this study we used 16 Wistar albino rats weighing between 220–240 g. The experimental animals were randomly divided into two groups: the model group (group M, n: 8) and control group (group C, n: 8). The necessary anesthesia was administered. Passing through a median laparotomy incision, the abdomen was entered and in group M and C the esophagus was freed and held by a suture at the gastroesophageal junction. After this procedure, about 1 cm proximal to the gastroesophageal junction, the esophagus was suspended by a suture. The esophagus segment between the two sutures was exposed to 0.1 mL 10% NaOH in group M and 0.1 mL saline in group C for 20 s. Ten days later all experimental animals were sacrificed and their esophagus removed. After dying with hematoxylin and eosine trichrome, the histopathological evaluation results for the rats in all groups were investigated with a light microscope.
Results. Histopathological examination indicated submucosal collagen increase, damage to muscularis mucosa and tunica muscularis and collagen deposition. In the model group, the rats had high neutrophils and tissue damage accompanied by necrosis. In the control group, the rats had minimal or no tissue damage and fibrosis was not observed.
Conclusion. Our procedure is relatively less invasive and easy to apply with corrosive esophagitis only in the required region, and at the same time treatment medications can be easily administered.

Key words

experimental model, corrosive esophagitis, NaOH, tracheal aspiration.

References (13)

  1. Larios-Arceo F, Ortiz GG, Huerta M, Leal-Cortés C, Saldaña JA, Bitzer-Quintero OK, Rodríguez-Reynoso S: Protective effects of melatonin against caustic esophageal burn injury in rats. J Pineal Res 2008, 45, 219–223.
  2. De Jong AL, Macdonald R, Ein S, Forte V, Turner A: Corrosive esophagitis in children: a 30-year review. Int J Pediatr Otorhinolaryngol 2001, 57, 203–211.
  3. Karnak I, Tanyel FC, Büyükpamukçu N, Hiçsönmez A: Combined use of steroid, antibiotics and early bougienage against stricture formation following caustic esophageal burns. J Cardiovasc Surg (Torino) 1999, 40, 307–310.
  4. Mutaf O: Treatment of corrosive esophageal strictures by long-term stenting. J Pediatr Surg 1996, 31, 681–685.
  5. Gehanno P, Guedon C: Inhibition of experimental lye stricture by penicillamine. Arch Otolaryngol 1981, 107, 145–147.
  6. Liu AJ, Richardson MA: Effects of N-acetylcysteine on experimentally induced esophageal lye injury. Ann Rhinol Laryngol 1985, 94, 477–482.
  7. Senturk E, Sen S, Pabuccu E, Unsal C, Meteoglu I: New experimental corrosive esophagitis model in rats. Pediatr Surg Int 2010, 26, 257–261.
  8. Lupa M, Magne J, Guarisco JL, Amedee R: Update on the diagnosis and treatment of caustic ingestion. Ochsner J 2009, 9, 54–59.
  9. Özçelik MF, Pekmezci S, Saribeyoğlu K, Unal E, Gümüştaş K, Doğusoy G: The effect of halofuginone, a specific inhibitor of collagen type 1 synthesis, in the prevention of esophageal strictures related to caustic injury. Am J Surg 2004, 187, 257–260.
  10. Holinger LD: Caustic osephagial burn. In: Swenson’s Pediatric Surgery. Ed: Raffensperger JG, Apleton and Lange, Connecticut 1990, 5th ed., 827–831.
  11. Hebel R, Stromberg MW: Anatomy of the laboratory rat. Williams & Wilkins, Baltimore 1976, 43–52.
  12. Cochran ST, Fonkalsrud EW, Gyepes MT: Complete obstruction of the gastric antrum in children following acid ingestion. Arch Surg 1978, 113, 308–312.
  13. Koltuksuz U, Mutus HM, Kutlu R, Ozyurt H, Cetin S, Karaman A Gürbüz N, Akyol O, Aydin NE: Effects of caffeic acid phenethyl ester and epidermal growth factor on the development of caustic esophageal stricture in rats. J Pediatr Surg 2001, 36, 1504–1509.
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