Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 4, July-August, p. 579–584

doi: 10.17219/acem/24503

Publication type: original article

Language: English

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Distribution in Rats Internal Organs of Intraperitoneally Given 125I-Labeled Heptapeptide [2-8]-Leucopyrokinin ([2-8]-LPK), a Truncated Analog of Insect Neuropeptide Leucopyrokinin

Florian Ryszka1,A,C,E, Barbara Dolińska1,B,C,E, Aleksandra Suszka-Świtek2,B,C, Monika Rykaczewska-Czerwińska3,A,B,C,D,F, Danuta Konopińska4,A,C,E, Mariola Kuczer4,B,C, Andrzej Plech5,A,B,C,D,F,*

1 Medical Higher School in Sosnowiec, Poland

2 Pharmaceutical Research and Production Plant, “Biochefa”, Sosnowiec, Poland

3 Department of Histology and Embriology, Medical University of Silesia, Katowice, Poland

4 Department of Toxicology and Health Protection, Medical University of Silesia, Katowice, Poland

5 Faculty of Chemistry, University of Wroclaw, Poland

Abstract

Background. It was previously found that synthetic, insect-derived octapeptide leucopyrokinin (LPK) applied directly into the lateral brain ventricle induced a significant antinociceptive effect in rats. Its synthetic truncated analog heptapeptide [2-8]-leucopyrokinin displayed a stronger antinociceptive effect in comparison to native LPK. Moreover it was previously found a high accumulation of these both 125I-labeled peptides in adrenals, as well as in hypothalamus and in hippocampus of rats brain.
Objectives. The aim of the present study was to assess the distribution of 125I-labeled [2-8]-leucopyrokinin in rats’ internal organs an in several parts of the brain after peripheral – intraperitoneal (i.p.) administration.
Material and Methods. The study was performed on male Wistar rats. A synthetic [2-8]-leucopyrokinin ([2-8]-LPK) was iodinated with Na125I. On the day of experiment a solution of 125I-[2-8]-LPK was i.p. injected and the next after 1 and 24 h animals were sacrificed by decapitation. Radioactivity levels in samples of parts of the brain and of internal organs were determined by counter Gamma Auto Count.
Results. A uniform, low accumulation 125I-[2-8]-LPK was found in evaluated samples of the brain and in internal organs.
Conclusion. The results of the present study indicate a weak penetration into the brain and internal organs of intraperitoneally applied 125I-[2-8]-LPK in rats and correspond with previously determined weak biological effects of i.p. injected LPK and [2-8]-LPK.

Key words

[2-8]-leucopyrokinin, distribution in rat brain and internal organs, intraperitoneal administration.

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