Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.736
5-Year Impact Factor – 2.135
Index Copernicus  – 168.52
MEiN – 70 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

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Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 3, May-June, p. 429–436

doi: 10.17219/acem/43888

Publication type: original article

Language: English

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The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

Dusan Sokolovic1,A,B,C,D,E,F, Branka Djordjevic1,C,D,F, Gordana Kocic1,A,E,F, Tatjana J. Stoimenov1,A,C,F, Zoran Stanojkovic2,B,C, Danka M. Sokolovic2,B,F, Andrej Veljkovic1,D,G, Goran Ristic3,A,E,F, Milena Despotovic1,D,F, Dusan Milisavljevic4,B,F, Radmilo Jankovic5,B,F, Ivana Binic6,B,E,F

1 Department of Biochemistry, Faculty of Medicine, University of Nis, Serbia

2 Institute for Blood Transfusion, Nis, Serbia

3 Faculty of Electronic Engineering, University of Nis, Serbia

4 Department of Otorhinolaryngology, Faculty of Medicine, University of Nis, Serbia

5 Department of Anesthesiology and Intensive Care, Faculty of Medicine, University of Nis, Serbia

6 Clinic of Dermatology and Venereology, Nis Clinical Center, Nis, Serbia

Abstract

Background. Microwaves from mobile phones are one of the environmental toxicants that are capable of compromising male fertility by inducing oxidative stress and apoptosis in the testes. Melatonin is a lipophilic tryptophan indole amine and a potent antioxidant.
Objectives. The aim of the study was to evaluate the effect of melatonin treatment on oxidative stress parameters and DNA fragmentation in the testicular tissue of rats exposed to microwave radiation (4 h/day).
Material and Methods. Adult Wistar rats were divided in 4 groups: I – treated with saline; II – treated with melatonin; III – exposed to microwaves; IV – exposed to microwaves and treated with melatonin. The melatonin (2 mg/kg ip) was administered daily. The animals were sacrificed after 20, 40 and 60 days.
Results. Melatonin treatment prevented previously registered increases in malondialdehyde after only 20 days. Furthermore, it reversed the effects of microwave exposure on xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). However, neither protein carbonyl content nor catalase and alkaline Dnase activity were changed due to melatonin treatment.
Conclusion. Melatonin exerts potent antioxidant effects in the testes of rats exposed to microwaves by decreasing the intensity of oxidative stress; it also reduces DNA fragmentation.

Key words

melatonin, microwave radiation, testicular tissue, oxidative stress, DNase.

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