Advances in Clinical and Experimental Medicine
2015, vol. 24, nr 2, March-April, p. 213–218
Publication type: original article
Effects of Oleuropein on Serum Inflammatory Cytokines and Histopathological Changes in Rats with Pancreatitis
1 Department of General Surgery, Bozok University Faculty of Medicine, Yozgat, Turkey
2 Department of General Surgery, Ondokuz Mayis University Faculty of Medicine, Samsun, Turkey
3 Department of Biochemistry, Ondokuz Mayis University Faculty of Medicine, Samsun, Turkey
4 Department of Pathology, Ondokuz Mayis University Faculty of Medicine, Samsun, Turkey
5 Department of Public Health, Ondokuz Mayis University Faculty of Medicine, Samsun, Turkey
Objectives. Acute pancreatitis is a severe debilitating disease with high morbidity and mortality. The severity of the disease is largely due to the actions of various inflammatory mediators. No specific therapy is indicated for AP and its treatment remains largely supportive. This study investigated the effect of oleuropein on serum inflammatory cytokines and distant organ injury in rats with acute pancreatitis induced by bile-pancreatic duct obstruction.
Material and Methods. Thirty adult male Wistar rats were randomly assigned to 3 groups of 10 rats each: group I: sham, group II: pancreatitis and group III: pancreatitis + oleuropein. Rats in Group III received oleuropein 20 mg/kg intragastrically during 10 days before surgery. The rats were sacrificed at 24 h after surgery and venous blood, pancreatic and lung tissue samples were obtained for analysis.
Results. A statistically significant difference was found between groups regarding serum amylase, LDH, lipase, pancreatic amylase, IL-6, IL-10 and TNF-α values with a Kruskal-Wallis test (p < 0.05). No statistically significant difference was found between Group II and III regarding pairwise comparison of amylase, LDH, lipase, pancreatic amylase, IL-6, IL-10 and TNF-α value by using pairwise comparisons with a Mann-Whitney U test (p > 0.01). A statistically significant difference between groups was found regarding edema, inflammation, hemorrhage and acinar cell necrosis in histopathological examination of the pancreas (p < 0.05). No difference was found between group II and III (p > 0.01). A difference was found between the groups in terms of lung tissue edema (p < 0.05) but no difference was found regarding hemorrhage and inflammation (p > 0.05). No statistically significant difference was found between group II and III with pairwise comparison (p > 0.01).
Conclusion. The experimental model used in this study showed to be appropriate for creation of acute pancreatitis. It was concluded that oleuropein as a prophylactic treatment has no protective effect on serum proinflammatory and anti-inflammatory cytokines as well as pancreatic tissue.
acute pancreatitis, oleuropein, cytokines, pancreatic damage.
- Horst NL, Marques RG, Diestel CF, Matzke BD, Caetona CER, Simões FC: Effects of Probiotic Supplementation on Markers of Acute pancreatitis in Rats. Curr Ther Res Clin Exp 2009, 70, 136–148.
- Baddeley RNB, Skipworth JRA, Pereira SP: Acute pancreatitis. Medicine 2011, 39, 108–115.
- Muhs BE, Patel S, Yee H, Marcus S, Shamamian P: Inhibition of Matrix Metalloproteinases Reduces Local and Distant Organ Injury Following Experimental Pancreatitis. J Surg Res 2003, 109, 110–117.
- Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD: Effects of gabexate mesilate on serum inﬂammatory cytokines in rats with acute necrotizing pancreatitis. Cytokine 2006, 33, 95–99.
- Karen M, Yuksel O, Akyürek N, Ofluoğlu E, Caglar K, Sahin TT, Pasaoglu H, Memis L, Akyürek N, Bostanci H: Probiotic Agent Saccharomyces boulardii Reduces the Incidence of Lung Injury in Acute Necrotizing Pancreatitis Induced Rats. J Surg Res 2010, 160, 139–144.
- van Santvoort HC, Besselink MG, Timmerman HM, van Minnen LP, Akkermans LM, Gooszen HG: Probiotics in surgery. Surgery 2008, 143, 1–7.
- Besselink MG, van Santvoort HC, Buskens E, Boermeester MA, van Goor H, Timmerman HM, Nieuwenhuijs VB, Bollen TL, van Ramshorst B, Witteman BJ, Rosman C, Ploeg RJ, Brink MA, Schaapherder AF, Dejong CH: Probiotic prophylaxis in predicted severe acute pancreatitis: a randomised, double-blind, placebo-controlled trial. Lancet 2008, 371, 651–659.
- Leung PS, Ip SP: Pancreatic acinar cell: its role in acute pancreatitis. Int J Biochem Cell Biol 2006, 38, 1024–1030.
- Andreadou I, Iliodromitis EK, Mikros E, Constantinou M, Agalias A, Magiatis P, Skaltsounis AL, Kamber E, Tsantili-Kakoulidou A, Kremastinos DT: The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. J Nutr 2006, 136, 2213–2219.
- Omar SH: Oleuropein in Olive and its Pharmacological Effects. Sci Pharm 2010, 78, 133–154.
- Medina E, de Castro A, Romero C, Brenes M: Comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity. J Agric Food Chem 2006, 54, 4954–4961.
- Bourboullos EJG, Geladopoulos T, Chrisofos M, Koutoukas J, Vassiliaids J: Oleuropein: A novel immunomodulator conferring prolonged survival in experimental sepsis by pseudomonas aeruginosa. Shock 2006, 26, 410–416.
- Meyerholz DK, Samuel I: Morphologic characterization of early ligation-induced acute pancreatitis in rats. Am J Surg 2007,194, 652–658.
- Ramudo L, Manso MA, Vicente S, De Dios I: Proand anti-inflammatory response of acinar cells during acute pancreatitis. Effect of N-acetyl cysteine. Cytokine 2005, 32, 125–131.
- Schütte K: Markers for predicting severity and progression of acute pancreatitis. Best Pract Res Cl Ga 2008, 22, 75–90.
- Brunicardi FC: Schwartz’s Principles of Surgery. 9th Edition 2010, 1179–1181.
- Weylandt KH, Nadolny A, Kahlke L, Köhnke T, Schmöcker C, Wang J, Lauwers GY: Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis. BBA 2008, 1782, 634–641.
- Miles EA, Zoubouli P, Calder PC: Differential anti-inflammatory effects of phenolic compounds from extra virgin olive oil identified in human whole blood cultures. Nutrition 2005, 21, 389–394.
- Motilva V, Sánchez-Fidalgo S, Barranco MD, Herrerías JM, Alarcón de la Lastra C: Mechanisms of increased gastric protection after NSAID-administration in rats consuming virgin olive oil diets. Eur e-J Clin Nutr Metab 2008, 3, 9–16.
- Turkyilmaz S, Alhan E, Ercin C, Kural Vanizor B, Kaklikkaya N, Ates B, Erdogan S, Topaloglu S: Effects of Caffeic Acid Phenethyl Ester on Pancreatitis in Rats. J Surg Res 2008, 145, 19–24.
- Zhao X, Andersson R, Wang X, Dib M, Wang X: Acute pancreatitis-associated lung injury: Pathophysiological mechanisms and potential future therapies. Scand J Gastroenterol 2002, 37, 1351–1358.