Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 5, September-October, p. 715–721

Publication type: original article

Language: English

The Effect of Agomelatine on Oxidative Stress Induced with Ischemia/Reperfusion in Rat Ovaries

Omer E. Yapca1,A,F, Bunyamin Borekci2,A,F, Mehmet I. Turan3,A,D,F, Mine Gulapoglu4,B,C,F

1 Obstetrics and Gynecology, Sorgun State Hospital, Yozgat, Turkey

2 Department of Obstetrics and Gynecology, Ataturk University, Erzurum, Turkey

3 Department of Pediatrics, Ataturk University, Erzurum, Turkey

4 Department of Biochemistry, Ataturk University, Erzurum, Turkey

Abstract

Background. Ovarian ischemia and reperfusion can lead to serious and irreversible health problems.
Objectives. The aim of this study is to investigate the protective effect of agomelatine against ovarian ischemia/ /reperfusion injury in rats using biochemical methods.
Material and Methods. Thirty female rats were divided into three groups (the number of animals in each group = 10), a control group in which ischemia/reperfusion was established (IRC), an ischemia/reperfusion + agomelatine (IRA) group and a healthy group given a sham operation (SG). Total glutathione (tGSH) and malondialdehyde (MDA) levels and glutathione peroxidase
Results. Biochemical results revealed MDA levels of 19.1 ± 2.03, 5.8 ± 1.5 and 5.5 ± 1.4 µmol/g protein in ovarian tissue in the IRC, IRA and SG groups, respectively. MPO activity in the IRC, IRA and SG groups was 7.87 ± 2.7, 4.0 ± 2.0 and 3.0 ± 1.0 U/g protein, respectively. tGSH levels were 1.87 ± 1.13, 4.37 ± 1.4 and 5.87 ± 1.64 nmol/g protein, respectively. GPx activity in the IRC, IRA and SG groups was 7.37 ± 1.68, 18.6 ± 3 and 17.75 ± 3.2 U/g protein, and SOD activity 31.1 ± 2.9, 45.3 ± 3.7 and 54 ± 4.2 U/g protein, respectively. The level of 8-OH/ /Gua, a product of DNA damage, was 2.18 ± 0.2 pmol/L in the IRC group, 1.28 ± 0.2 pmol/L in the IRA group and 0.93 ± 0.01 pmol/L in the SG group.
Conclusion. Agomelatine prevented ovarian ischemia/reperfusion injury

Key words

agomelatine, rat, ovary, antioxidants, ischemia, reperfusion.

References (30)

  1. Beaunoyer M, Chapdelaine J, Bouchard S, Ouimet A: Asynchronous bilateral ovarian torsion. J Pediatr Surg 2004, 39, 746–749.
  2. Celik A, Ergun O, Aldemir H, Ozcan C, Ozok G, Erdener A, Balyk E: Long-term results of conservative management of adnexal torsion in children. J Pediatr Surg 2005, 40, 704–708.
  3. Ingec M, Isaoglu U, Yilmaz M, Calik M, Polat B, Alp HH, Kurt A, Gundogdu C, Suleyman H: Prevention of ischemia-reperfusion injury in rat ovarian tissue with the on-off method. J Physiol Pharmacol 2011, 62, 575–582.
  4. Etensel B, Ozkisacik S, Ozkara E, Karul A, Oztan O, Yazici M, Gursoy H: Dexpanthenol attenuates lipid peroxidation and testicular damage at experimental ischemia and reperfusion injury. Pediatr Surg Int 2007, 23, 177–181.
  5. Isaoglu U, Yilmaz M, Calik M, Polat B, Bakan E, Kurt A, Albayrak Y, Suleyman H: Biochemical and histopathological investigation of the protective effect of disulfiram in ischemia-induced ovary damage. Gynecol Endocrinol 2012, 28, 143–147.
  6. Ingec M, Calik M, Gundogdu C, Kurt A, Yilmaz M, Isaoglu U, Salman S, Akcay F, Suleyman H: Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of IschemiaReperfusion in Rats. Int J Fertil & Steril 2012, 6, 19–26.
  7. Zlotos DP: Recent advances in melatonin receptor ligands. Arch Pharm (Weinheim) 2005, 338, 229–247.
  8. Turkoz Y, Celik O, Hascalik S, Cigremis Y, Hascalik M, Mizrak B, Yologlu S: Melatonin reduces torsion-detorsion injury in rat ovary: biochemical and histopathologic evaluation. J Pineal Res 2004, 37, 137–141.
  9. Song Y, Chan CW, Brown GM, Pang SF, Silverman M: Studies of the renal action of melatonin: evidence that the effects are mediated by 37 kDa receptors of the Mel1a subtype localized primarily to the basolateral membrane of the proximal tubule. Faseb J 1997, 11, 93–100.
  10. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979, 95, 351–358.
  11. Wei H, Frenkel K: In vivo formation of oxidized DNA bases in tumor promoter-treated mouse skin. Cancer Res 1991, 51, 4443–4449.
  12. Sedlak J, Lindsay RH: Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968, 25, 192–205.
  13. Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967, 70, 158–169.
  14. Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clinical Chemist 1988, 34, 497–500.
  15. Shigenaga MK, Aboujaoude EN, Chen Q, Ames BN: Assays of oxidative DNA damage biomarkers 8-oxo-2’- -deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluids by high-performance liquid chromatography with electrochemical detection. Meth Enzymol 1994, 234, 16–33.
  16. Kaur H, Halliwell B: Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochem J 1996, 318 (Pt 1), 21–23.
  17. Cohen SB, Oelsner G, Seidman DS, Admon D, Mashiach S, Goldenberg M: Laparoscopic detorsion allows sparing of the twisted ischemic adnexa. J Am Assoc Gynecol Laparosc 1999, 6, 139–143.
  18. Eckler K, Laufer MR, Perlman SE: Conservative management of bilateral asynchronous adnexal torsion with necrosis in a prepubescent girl. J Pediatr Surg 2000, 35, 1248–1251.
  19. Duntan C: Torsion of the ovary. J. B. Lippincott Company, Philadelphia 1994.
  20. Gutteridge JM: Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995, 41, 1819– –1828.
  21. Girotti AW: Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998, 39, 1529–1542.
  22. Lavelli V, Peri C, Rizzolo A: Antioxidant activity of tomato products as studied by model reactions using xanthine oxidase, myeloperoxidase, and copper-induced lipid peroxidation. J Agric Food Chem 2000, 48, 1442–1448.
  23. Kumbasar S, Yapca O, Bilen H, Suleyman B, Ozgeris F, Borekci B, Suleyman H: The Effect of Lacidipine on Ischemia-Reperfusion Induced Oxidative Damage in Ovaries of Female Rats. Biomed Res 2012, 23, 23–26.
  24. Rangan U, Bulkley GB: Prospects for treatment of free radical-mediated tissue injury. Br Med Bull 1993, 49, 700–718.
  25. Di Mascio P, Murphy ME, Sies H: Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr 1991, 53, Suppl 1, 194–200.
  26. Kurt A, Ingec M, Isaoglu U, Yilmaz M, Cetin N, Calik M, Polat B, Akcay F, Gundogdu C, Suleyman H: An investigation about the inhibition of acute ischemia/reperfusion damage by dexmedetomidine in rat ovarian tissue. Gynecol Endocrinol 2012.
  27. Coskun KA GA, Halici Z, Oral A, Seyrek M, Bayir Y, Kilic C, Yigit T, Ozer T, Uzar AI: The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion. EAJM 2011, 43, 33–38.
  28. Ceretta LB, Reus GZ, Abelaira HM, Ribeiro KF, Zappellini G, Felisbino FF, Steckert AV, Dal-Pizzol F, Quevedo J: Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan-induced diabetic rats. Exp Diabetes Res 2012, 2012, 302682.
  29. Drevet JR: The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol 2006, 250, 70–79.
  30. Grollman AP, Moriya M: Mutagenesis by 8-oxoguanine: an enemy within. Trends Genet 1993, 9, 246–249.
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