Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 4, July-August, p. 523–529

Publication type: original article

Language: English

The Distribution of Beta Lactamase Genes in Escherichia Coli Phylotypes Isolated from Diarrhea and UTI Cases in Northwest Iran

Zahra Hemati1,B,C,D, Reza Ghanbarpour1,A,E,F, Hesam Alizade2,C,D,E,F

1 Molecular Microbiology Department, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran

2 Department of Microbiology, International Branch, Shahid Beheshti University of Medical Science, Tehran, Iran

Abstract

Background. Pathogenic Escherichia coli strains are a common cause of intestinal and extra-intestinal infections, especially in developing countries. Extended spectrum beta-lactamases (ESBLS), a heterogeneous group of plasmidencoded beta-lactamases, are common throughout the world.
Objectives. The aim of the present study was to determine the phenotypic and genotypic characteristics of ESBLS produced by E. coli isolates taken from patients with diarrhea and urinary tract infections (UTI) in northwest Iran.
Material and Methods. A total of 132 E. coli isolates (92 isolates from UTI and 40 isolates from diarrheic cases) were recovered and confirmed by biochemical tests. The isolates were examined for blaTEM and blaSHV genes and phylogenetic background by two multiplex PCR assays. The isolates were tested for antibiotic susceptibility against nine antibiotic agents by the disk diffusion method.
Results. The phylogenetic analysis showed that the UTI isolates mostly fell into phylo-group B2, followed by D, while the diarrheic isolates belonged to phylo-groups D and A. Out of 92 UTI isolates, 29.3% and 17.4% possessed blaTEM and blaSHV genes, respectively. Ten diarrheic isolates were positive for blaTEM, two isolates possessed the blaSHV gene, and one isolate was positive for both genes. The UTI isolates that were positive for blaTEM and blaSHV genes mostly belonged to phylo-groups D and B2, whereas the diarrhea isolates were in phylo-groups D and A. Phylogenetic group D isolates have an accumulation of ESBLS genes in the diarrheic and UTI isolates. In both the UTI and diarrhea isolates, the maximum rate of resistance was against cefazolin, and the minimum rate of resistance was against nitrofurantoin. Twenty-four antibiotic resistance patterns were observed among the isolates. The amikacin, ciprofloxacin, cefotaxime, cefuroxime, cefazolin, gentamicin, nalidixic acid and trimethoprim/sulfamethoxazole resistance pattern was the most prevalent in the isolates that belonged to phylo-group D.
Conclusion. The correct choice of effective antibiotic policy is needed to limit the spread of antibiotic resistance in bacteria.

Key words

extended spectrum beta-lactamases (ESBLs), Escherichia coli, diarrhea, UTI, phylogeny.

References (30)

  1. Leimbach A, Hacker J, Dobrindt U: E. coli as an all-rounder: The thin line between commensalism and pathogenicity. Curr Top Microbiol Immunol 2013, 358, 3–32.
  2. Oliveira FA, Paludo KS, Arend LNV, Farah SMSS, Pedrosa FO, Souza EM, Surek M, Picheth G, Fadel-Picheth CMT: Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains. Genet Mol Res 2011, 10, 4114–4125.
  3. Pitout JD: Extraintestinal pathogenic Escherichia coli: A combination of virulence with antibiotic resistance. Front Microbiol 2012, 3.
  4. Katouli M: Population structure of gut Escherichia coli and its role in development of extra-intestinal infections. Iran J Microbiol 2010, 2, 59–72.
  5. Carvalho VM, Osugui L, Setzer AP, Lopez RPG, Pestana de Castro AF, Irino K, Catão-Dias JL: Characterization of extraintestinal pathogenic Escherichia coli isolated from captive wild felids with bacteremia. J Vet Diagn Invest 2012, 24, 1014–1016.
  6. Moreno AC, Filho AF, Gomes TAT, Ramosc STS, Montemora LPG, Tavaresa VC, Filho LS, Irinoe K, Martineza MB: Etiology of childhood diarrhea in the northeast of Brazil: significant emergent diarrheal pathogens. Diagn Microbiol Infect Dis 2010, 66, 50–57.
  7. Banu A, Kabbin J, Anand M: Extraintestinal infections due to Escherichia coli: an emerging issue. J Clin Diag Res 2011, 5, 486–490.
  8. Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH: Genotypic versus phenotypic methods to detect extended-spectrum beta-lactamases (ESBLs) in Uropathogenic Escherichia coli. Ann Biol Res 2012, 3, 2454–2458.
  9. Brown NG, Pennington JM, Huang W, Ayvaz T, Palzkill T: Multiple global suppressors of protein stability defects facilitate the evolution of extended-spectrum TEM β-lactamases. J Mol Biol 2010, 404, 832–846.
  10. Ojer-Usoz E, Gonzalez D, Vitas AI, Leiva J, García-Jalón I, Febles-Casquero A, Escolano Mde L: Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae in meat products sold in Navarra, Spain. Meat Sci 2013, 93, 316–321.
  11. Isendahl J, Turlej-Rogacka A, Manjuba C, Rodrigues A, Giske CG, Naucler P: Fecal carriage of ESBL-producing E. coli and K. pneumoniae in children in Guinea-Bissau: a hospital-based cross-sectional study. PLoS One 2012, 7, 51981.
  12. Escobar-Paramo P, Grenet K, Le Menac’h A, Rode L, Salgado E, Amorin C, Gouriou S, Picard B, Rahimy MC, Andremont A, Denamur E, Ruimy R: Large-scale population structure of human commensal Escherichia coli isolates. Appl Environ Microbiol 2004, 70, 5698–5700.
  13. Asai T, Masani K, Sato C, Hiki M, Usui M, Baba K, Ozawa M, Harada K, Aoki H, Sawada T: Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan. Acta Vet Scand 2011, 53.
  14. Sharma J, Sharma M, Ray P: Detection of TEM & SHV genes in Escherichia coli & Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res 2010, 132, 332–336.
  15. Clermont O, Bonacorsi S, Bingen E: Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2000, 66, 4555–4558.
  16. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: twenty-second. Wayne, PA, USA 2012, 22th informational supplement, M100–S22.
  17. Franiczek R, Sobieszczanska B, Turniak M, Kasprzykowska U, Krzyzanowska B, Jermakow K, Mokracka-Latajka G: ESBL-producing Escherichia coli isolated from children with acute diarrhea-antimicrobial susceptibility, adherence patterns and phylogenetic background. Adv Clin Exp Med 2012, 21, 187–192.
  18. Gayathri D, Eramma NK, Devaraja TN: New Delhi metallo beta-Lactamase-1; Incidence and threats. Int J Biol Med Res 2012, 3, 1870–1874.
  19. Nasehi N, Shahcheraghi F, Nikbin VS, Nematzadeh S: PER, CTX-M, TEM and SHV beta-lactamases in clinical isolates of Klebsiella pneumonia isolated from Tehran, Iran. Iran J Basic Med Sci 2010, 13.
  20. Ghanbarpour R: Detection of β-lactamase and urovirulence genes in Escherichia coli serogroups isolated from urinary tract infection in cats. Comp Clin Pathol 2013, 22, 591–596.
  21. Lahlaoui H, Anis BH, Mohamed K, Mohamed BM: Emergence of SHV-12 extended spectrum beta-lactamase among clinical isolates of Enterobacter cloacae in Tunisia. Microb Pathog 2012, 53, 64–65.
  22. Shahid M, Singh A, Sobia F Rashid M, Malik A, Shukla I, Khan HM: blaCTX-M, blaTEM, and blaSHV in Enterobacteriaceae from North-Indian tertiary hospital: high occurrence of combination genes. Asian Pac J Trop Med 2011, 4, 101–105.
  23. Mirsalehian A, Akbari-Nakhjavani F, Peymani A, Kazemi B, Jabal Ameli F, Mirafshar SM: Prevalence of extended spectrum β-lactamase-producing Enterobacteriaceae by phenotypic and genotypic methods in intensive care units in Tehran, Iran. DARU 2008, 16.
  24. Akpaka PE, Legall B, Padman J: Molecular detection and epidemiology of extended-spectrum beta-lactamase genes prevalent in clinical isolates of Klebsiella pneumoniae and E. coli from Trinidad and Tobago. West Indian Med J 2010, 59, 591–596.
  25. Herindrainy P, Randrianirina F, Ratovoson R, Ratsima Hariniana E, Buisson Y, Genel N, Decré D, Arlet G, Talarmin A, Richard V: Rectal carriage of extended-spectrum beta-lactamase-producing gram-negative bacilli in community settings in Madagascar. PLoS One 2011, 6, e22738.
  26. Wu JH, Chiou YH, Chang JT, Wang HP, Chen YY, Hsieh KS: Urinary tract infection in infants: a single-center clinical analysis in southern Taiwan. Pediatr Neonatol 2012, 53, 283–288.
  27. Hawser SP, Badal RE, Bouchillon SK, Hoban DJ, Biedenbach DJ, Cantón R, Paterson DL: Monitoring the global in vitro activity of ertapenem against Escherichia coli from intra-abdominal infections: SMART 2002–2010. Int J Antimicrob Agents 2013, 41, 224–228.
  28. Birgy A, Cohen R, Levy C, Bidet P, Courroux C, Benani M, Thollot F, Bingen E: Community faecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae in French children. BMC Infect Dis 2012, 12.
  29. Abdou E, Daoud Z, Roula AM: Leaf and branch extracts of Eriobotrya japonica extracts antibacterial activity against ESBL-producing Escherichia coli and Klebsiella pneumoniae. Int J Phytomedicine 2011, 3, 120–128.
  30. Shanthi M, Sekar U: Extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae: risk factors for infection and impact of resistance on outcomes. J Assoc Physicians India 2010, 58, 41–44.
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