Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
5-Year IF – 2.0, IF – 1.9, JCI (2024) – 0.43
Scopus CiteScore – 4.3
Q1 in SJR 2024, SJR score – 0.598, H-index: 49 (SJR)
ICV – 161.00; MNiSW – 70 pts
Initial editorial assessment and first decision within 24 h

ISSN 1899–5276 (print), ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 4, July-August, p. 505–509

Publication type: original article

Language: English

The Impact of Morin, a Natural Flavonoid, on Cyclophosphamide-Induced Changes in the Oxidative Stress Parameters in Rat Livers

Anna Merwid-Ląd1,A,B,C,D, Małgorzata Trocha1,B,C, Ewa Chlebda-Sieragowska1,C,D, Tomasz Sozański1,E, Marta Szandruk1,D, Jan Magdalan1,E, Dorota Ksiądzyna1,D, Małgorzata Pieśniewska1,B,G, Lidia Fereniec-Gołębiewska1,B,G, Joanna Kwiatkowska1,B,G, Adam Szeląg1,F

1 Department of Pharmacology, Wroclaw Medical University, Poland

Abstract

Background. Cyclophosphamide (CPX) has many adverse effects, partly due to oxidative stress induction in various tissues. Morin is one of the natural flavonoids with strong antioxidant properties.
Objectives. The aim of the current research was to estimate the influence of morin on changes in antioxidant parameters in rat livers after cyclophosphamide administration.
Material and Methods. The study was performed on Wistar rats. The rats in Group C received 0.9% saline; those in Group CX received cyclophosphamide (CPX); and those in Group M-CX received CPX with morin. Cyclophosphamide and morin were given by gastric gavage for 10 consecutive days at doses of 15 mg/kg and 100 mg/kg, respectively. Malondialdehyde (MDA) and glutathione (GSH) concentrations, superoxide dismutase (SOD) activity and catalase (CAT) activity were determined in liver tissue homogenates.
Results. CPX caused a significant decrease in SOD activity and GSH levels, but only the latter was fully restored by morin. There were no significant differences in CAT activity in the various groups. CPX also insignificantly decreased MDA levels, which was aggravated by co-administration of morin.
Conclusion. The results obtained indicate that morin may exert some protective action on CPX-induced changes in the antioxidant state in rat livers.

Key words

cyclophosphamide, morin, oxidative stress, liver, rat.

References (22)

  1. Abraham P, Rabi S: Protective effect of aminoguanidine against cyclophosphamide-induced oxidative stress and renal damage in rats. Redox Rep 2011, 16, 8–14.
  2. Korkmaz A, Topal T, Oter S: Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis; implication of reactive oxygen and nitrogen species as well as PARP activation. Cell Biol Toxicol 2007, 23, 303–312.
  3. Topal T, Oztas Y, Korkmaz A, Sadir S, Oter S, Coskun O, Bilgic H: Melatonin ameliorates bladder damage induced by cyclophosphamide in rats. J Pineal Res 2005, 38, 272–277.
  4. Askar I, Oktay MF, Gurlek A, Bac B: Protective effects of some antineoplastic agents on ischemia-reperfusion injury in epigastric island skin flaps. Microsurgery 2006, 26, 193–199.
  5. Merwid-Ląd A, Trocha M, Chlebda E, Sozański T, Magdalan J, Ksiądzyna D, Kopacz M, Kuźniar A, Nowak D, Pieśniewska M, Fereniec-Gołębiewska L, Kwiatkowska J, Szeląg A: Effects of morin-5’-sulfonic acid sodium salt (NaMSA) on cyclophosphamide-induced changes in oxido-redox state in rat liver and kidney. Hum Exp Toxicol 2012, 31, 812–819.
  6. Ray S, Chowdhury P, Pandit B, Ray SD, Das S: Exploring the antiperoxidative potential of morin on cyclophosphamide and flutamide-induced lipid peroxidation and changes in cholesterol profile in rabbit model. Acta Pol Pharm 2010, 67, 35–44.
  7. Karthik Kumar V, Vennila S, Nalini N: Modifying effects of morin on the development of aberrant crypt foci and bacterial enzymes in experimental colon cancer. Chem Toxicol 2009, 47, 309–315.
  8. Sreedharan V, Venkatachalam KK, Namasivayam N: Effect of morin on tissue lipid peroxidation and antioxidant status in 1, 2-dimethylhydrazine induced experimental colon carcinogenesis. Invest New Drugs 2009, 27, 21–30.
  9. Zhang R, Kang KA, Piao MJ, Maeng YH, Lee KH, Chang WY, You HJ, Kim JS, Kang SS, Hyun JW: Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. Chem Biol Interact 2009, 177, 21–27.
  10. Merwid-Ląd A, Trocha M, Chlebda E, Sozański T, Magdalan J, Ksiądzyna D, Pieśniewska M, Szeląg A: The effects of morin, a naturally occurring flavonoid, on cyclophosphamide-induced toxicity in rats. Adv Clin Exp Med 2011, 20, 683–690.
  11. Johanson LH, Borg HLA: A spectrophotometric method for determination of catalase activity in small tissue sample. Anal Biochem 1988, 174, 331–336.
  12. Kim SH, Lee IC, Lim JH, Moon C, Bae CS, Kim SH, Shin DH, Park SC, Kim HC, Kim JC: Protective effects of pine bark extract on developmental toxicity of cyclophosphamide in rats. Food Chem Toxicol 2012, 50, 109–115.
  13. Tripathi DN, Jena GB: Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage: a study in mice. Chem Biol Interact 2009, 180, 398–406.
  14. Tripathi P, Tripathi R, Patel RK, Pancholi SS: Investigation of antimutagenic potential of Foeniculum vulgare essential oil on cyclophosphamide induced genotoxicity and oxidative stress in mice. Drug Chem Toxicol 2013, 36, 35–41.
  15. Korkmaz A, Topal T, Oter S: Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis; implication of reactive oxygen and nitrogen species as well as PARP activation. Cell Biol Toxicol 2007, 23, 303–312.
  16. Oboh G, Akomolafe TL, Adefegha SA, Adetuyi AO: Attenuation of cyclophosphamide-induced neurotoxicity in rat by yellow dye extract from root of Brimstone tree (Morinda lucida). Exp Toxicol Pathol 2012, 64, 591–596.
  17. Al-Yahya AA, Al-Majed AA, Gado AM, Daba MH, Al-Shabanah OA, Abd-Allah AR: Acacia Senegal gum exudate offers protection against cyclophosphamide-induced urinary bladder cytotoxicity. Oxid Med Cell Longev 2009, 2, 207–213.
  18. Kok LD, Wong YP, Wu TW, Chan HC, Kwok TT, Fung KP: Morin hydrate: a potential antioxidant in minimizing the free-radicals-mediated damage to cardiovascular cells by anti-tumor drugs. Life Sci 2000, 67, 91–99.
  19. Hong SS, Jin MJ, Han HK: Enhanced systemic availability of methotrexate in the presence of morin in rats. Biopharm Drug Dispos 2008, 29, 189–193.
  20. Fang SH, Hou YC, Chao PD: Pharmacokinetic and pharmacodynamic interactions of morin and cyclosporin. Toxicol Appl Pharmacol 2005, 205, 65–70.
  21. Król W, Dworniczak S, Pietsz G, Czuba ZP, Kunicka M, Kopacz M, Nowak D: Synthesis and tumoricidal activity evaluation of new morin and quercetin sulfonic derivatives. Acta Pol Pharm 2002, 59, 77–79.
  22. Sahu SC, Gray GC: Lipid peroxidation and DNA damage induced by morin and naringenin in isolated rat liver nuclei. Food Chem Toxicol 1997, 35, 443–447.
© Wroclaw Medical University