Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 2, March-April, p. 215–223

Publication type: original article

Language: English

Gene-Gene Interaction of ACE I/D, Endothelial Nitric Oxide Synthase 4 a/b and ApoE does not Affect Coronary Artery Disease Severity

Selçuk Matyar1,A,B,D,E,F, Esmeray Acartürk2,A,D,E, Gülen Attila1,A,B,D, İlker Ünal3,C, Levent Soyer2,B, Onur Akpınar2,B,E,F

1 Department of Biochemistry, Çukurova University, Adana, Turkey

2 Department of Cardiology, Çukurova University, Adana, Turkey

3 Department of Biostatistics, Çukurova University, Adana, Turkey

Abstract

Objectives. Previous studies have shown the impact of angiotensin converting enzyme (ACE) insertion/deletion (I/D), endothelial nitric oxide synthase (eNOS) polymorphisms and ApoE genotypes on coronary artery disease (CAD). The aim of this study is to investigate the relationship between the genetic polymorphisms and the severity of CAD and to evaluate their potential interactions.
Material and Methods. All patients underwent coronary angiography; coronary score (CS) and severity score (SS) were calculated for them. ACE I/D, eNOS and ApoE polymorphisms were detected by polymerase chain reaction (PCR).
Results. Neither CS nor SS showed a direct relationship with eNOS and ApoE genotypes. CS and SS were found to be high in patients carrying the ACE DD allele (p = 0.034 and p = 0.009). In the gene interactions, there was an increase in the SS only in patients with coexisting eNOS b/b genotype and ACE DD allele (p = 0.043).
Conclusion. The interactions of the gene polymorphisms investigated don’t play an important role in determining an individual’s risk for the severity of CAD.

Key words

ACE gene, ApoE gene, eNOS gene, gene–gene interaction, severity of coronary artery disease.

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