Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2012, vol. 21, nr 6, November-December, p. 727–733

Publication type: original article

Language: English

Estimating the Postmortem Interval by the Difference Between Oxidant/Antioxidant Parameters in Liver Tissue

Określanie czasu zgonu za pomocą różnicy między wskaźnikami oksydacyjnymi i antyoksydacyjnymi w tkance wątroby

Mustafa T. Sener1,A,D, Halis Suleyman2,A,D, Ahmet Hacimuftuoglu2,D, Beyzagul Polat2,B,E,F, Nihal Cetin2,B,E,F, Bahadir Suleyman2,B, Fatih Akcay3,C

1 Department of Forensic Medicine, Faculty of Medicine, Ataturk University, Erzurum, Turkey

2 Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

3 Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey

Abstract

Background. So far the authors could not find any published paper that shows the presence of a possibly significant relationship between oxidant/antioxidant parameters and time of death.
Objectives. This study sought to investigate whether the differences between the levels of endogenous parameters like superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione S-transferases (GST), total glutathione (GSH), catalase (CAT) and nitric oxide (NO) have a relationship with the early postmortem interval (EPI).
Material and Methods. A total of 60 Albino Wistar male rats weighing between 220–230 grams were obtained. The rats were divided into six subgroups (n = 10), fed at room temperature (220C) and then decapitated. The livers of the dead rats were extracted at intervals of one hour and biochemical examinations were performed and biochemical results obtained from the animal groups had been evaluated.
Results. The authors performed linear regression analysis in order to search for factors predicting the time of death. The time of death was taken as a dependent variable with SOD, MDA, GPx, GST, GSH, CAT, and NO as independent variables. This model resulted with a high predictive value (adjusted R square = 94.0%). SOD, GST and NO were found to be significant factors independently affecting the prediction of the death time.
Conclusion. In conclusion, the results obtained from the experiment showed that the oxidant and antioxidant parameters are important for estimating the EPI.

Streszczenie

Wprowadzenie. Dotychczas nie ukazała się publikacja, która opisywałaby potencjalnie znaczący związek między wskaźnikami oksydacyjnymi i antyoksydacyjnymi a czasem zgonu.
Cel pracy. Zbadanie, czy różnice między stężeniem wskaźników endogennych, takich jak dysmutaza ponadtlenkowa (SOD), aldehyd malonowy (MDA), peroksydaza glutationowa (GPx), glutation S-transferazy (GST), całkowity glutation (tGSH), katalaza (CAT) i tlenek azotu (NO) mają związek z wczesnym czasem zgonu (EPI).
Materiał i metody. Do badań włączono 60 szczurów rasy Albino szczepu Wistar płci męskiej o masie ciała 220–230 g. Szczury podzielono na 6 podgrup (n = 10), karmiono w temperaturze pokojowej (22oC) i uśmiercono za pomocą dekapitacji. Wątroby martwych szczurów ekstrahowano w odstępach godzinnych, przeprowadzono badanie biochemiczne i oceniono wyniki uzyskane w poszczególnych grupach zwierząt.
Wyniki. Przeprowadzono analizę regresji liniowej w celu znalezienia czynników, które pozwoliłyby przewidzieć czas zgonu. Czas zgonu przyjęto jako zmienną zależną, a SOD, MDA, GPx, GST, GSH, CAT i NO jako zmienne niezależne. Taki model miał dużą wartość prognostyczną (skorygowane R2 = 94,0%). SOD, GST i NO były czynnikami niezależnymi pozwalającymi na przewidywanie czasu zgonu.
Wnioski. Wyniki uzyskane w doświadczeniu wykazały, że wskaźniki oksydacyjne i antyoksydacyjne są przydatne do oceny wczesnego czasu zgonu.

Key words

early postmortem interval, oxidants, antioxidants, liver, rats

Słowa kluczowe

wczesny czas zgonu, utleniacze, przeciwutleniacze, wątroba, szczury

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