Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
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ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2012, vol. 21, nr 4, July-August, p. 433–439

Publication type: original article

Language: English

Lithospermic Acid and Ischemia/Reperfusion Injury of the Rat Small Intestine Prevention

Kwas litospermowy a zapobieganie niedokrwieniu/uszkodzeniu reperfuzyjnemu jelita cienkiego u szczura

Hulya Ozturk1,, Elcin Hakan Terzi2,, Ufuk Ozgen3,, Arif Duran4,, Hayrettin Ozturk1,

1 Department of Pediatric Surgery, Abant Izzet Baysal University, Medical School, Bolu, Turkey

2 Department of Histology and Embryology, Abant Izzet Baysal University, Medical School, Bolu, Turkey

3 Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey

4 Department of Emergency, Abant Izzet Baysal University, Medical School, Bolu, Turkey

Abstract

Background. Intestinal ischemia and reperfusion (I-R) injury of different causes, including cardiac insufficiency, sepsis, vasodepressant and cardiodepressant drugs, and complications of long-lasting surgery, represents a major clinical problem.
Objectives. The purpose of the present study was to investigate whether lithospermic acid (LA) can reduce oxidative stress and histological damage in the rat small bowel subjected to mesenteric I-R injury.
Material and Methods. The study was performed on three groups of animals, each composed of 7 rats: the SO (sham operation) group, the I-R/Untreated group and the I-R/LA (I-R plus LA pretreatment) group. Intestinal ischemia for 45 minutes and reperfusion for 60 minutes were applied. Ileum specimens were obtained to determine the tissue level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and myeloperoxidase (MPO) activities and histological changes.
Results. Untreated intestinal I-R resulted in increased tissue MDA and MPO levels and diminished SOD and GPx activities. These changes were found to be almost reversed in the LA treatment group. Histopathologically, the intestinal injury in rats treated with LA was less than the untreated I-R group.
Conclusion. Lithospermic acid attenuates mesenteric ischemia reperfusion injury in rat intestines by increasing tissue SOD and GPx activities and decreasing MDA and MPO levels. Lithospermic acid also improves morphological alterations which occurred after periods of reperfusion.

Streszczenie

Wprowadzenie. Uszkodzenie jelit spowodowane niedotlenieniem i reperfuzją (IR) wynikające z różnych przyczyn, w tym niewydolności serca, posocznicy, stosowania leków wazodepresyjnych i kardiodepresyjnych oraz powikłań dużych zabiegów chirurgicznych, jest ważnym problemem klinicznym.
Cel pracy. Zbadanie, czy kwas litospermowy (LA) może zmniejszyć stres oksydacyjny i uszkodzenie histologiczne jelita cienkiego u szczurów, u których wywołano niedotlenienie i reperfuzję krezki.
Materiał i metody. Badanie zostało przeprowadzone na trzech grupach zwierząt, z których każda składała się z 7 szczurów: grupa SO (operacja pozorowana), grupa IR nieleczona i grupa IR / LA (IR oraz podanie LA przed leczeniem). Niedokrwienie jelit utrzymywano przez 45 minut i reperfuzję przez 60 minut. Pobrano wycinki jelita krętego w celu określenia stężenia aldehydu malonowego (MDA), dysmutazy ponadtlenkowej (SOD), peroksydazy glutationowej (GPx), katalazy (CAT) i mieloperoksydazy (MPO) w tkankach i zmian histologicznych.
Wyniki. Nieleczone IR jelit spowodowało zwiększenie stężenia tkankowego MDA i MPO oraz mniejszą aktywność SOD i GPx. Zmiany te okazały się niemal odwrotne w grupie leczonej LA. Histopatologicznie uszkodzenie jelit u szczurów leczonych LA było mniejsze niż w grupie IR nieleczonej.
Wnioski. Podawanie kwasu litospermowego zmniejsza uszkodzenie jelit spowodowane niedotlenieniem i reperfuzją u szczurów przez zwiększenie tkankowej SOD i aktywności GPx, zmniejszenie stężenia MDA i MPO. Kwas litospermowy zmniejsza zmiany morfologiczne, które nastąpiły po okresie reperfuzji.

Key words

small bowel, ischemia and reperfusion injury, lithospermic acid

Słowa kluczowe

jelito cienkie, uszkodzenie spowodowane niedotlenieniem i reperfuzją, kwas litospermowy

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